Establishment of QuEChERS-UPLC-MS/MS method to determine three kinds of ochratoxins in coffee
<b>Objective：</b> A new method was developed for the simultaneous determination of three kinds of ochratoxins in coffee by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) combined with QuEChERS pretreatment. <b>Methods：</b> Samples were extr...
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The Editorial Office of Food and Machinery
2023-12-01
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| Series: | Shipin yu jixie |
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| Online Access: | http://www.ifoodmm.com/spyjxen/article/abstract/20231115 |
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| author | GAO Yunkai CHEN Xiaomei CHEN Chunqian ZHOU Lingyu DENG Yinglin YIN Qingchun |
| author_facet | GAO Yunkai CHEN Xiaomei CHEN Chunqian ZHOU Lingyu DENG Yinglin YIN Qingchun |
| author_sort | GAO Yunkai |
| collection | DOAJ |
| description | <b>Objective：</b> A new method was developed for the simultaneous determination of three kinds of ochratoxins in coffee by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) combined with QuEChERS pretreatment. <b>Methods：</b> Samples were extracted by acetonitri-water-formic acid solution (55∶40∶5) with ultrasonic, salted out with a QuEChERS salt pocket, and then purified with a (ZanChERS-Myco17) column. The chromatographic separation was performed on an ACQUITY UPLC BEH C<sub>18 </sub>(1.7 μm, 2.1 mm×100 mm) column by gradient elution using a mobile phase comprising 0.2% formic acid aqueous solution and acetonitrile. In the positive ion mode of electric spray, the samples detected were used by multiple reaction monitoring (MRM) modes with internal standard method. <b>Results：</b> Three target substances had a good linear relationship in the range of 0.1~20.0 ng/mL, and the correlation coefficient was greater than 0.999 46. The detection limit and quantitative limit were 0.1~0.2 μg/kg and 0.2~0.7 μg/kg, respectively. Average recoveries at three spiked levels in the coffee matrix were 79.0%~103.3% with a relative standard deviation of 1.5%~7.6%. <b>Conclusion：</b> This method has the advantages of simple sample pretreatment, good reproducibility and high efficiency, and is suitable for the high-throughput detection of multiple ochratoxins in different coffee samples. |
| format | Article |
| id | doaj-art-2176bccd4cab4d57b24e98ec56da60d9 |
| institution | OA Journals |
| issn | 1003-5788 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | The Editorial Office of Food and Machinery |
| record_format | Article |
| series | Shipin yu jixie |
| spelling | doaj-art-2176bccd4cab4d57b24e98ec56da60d92025-08-20T02:23:32ZengThe Editorial Office of Food and MachineryShipin yu jixie1003-57882023-12-013911919710.13652/j.spjx.1003.5788.2023.80084Establishment of QuEChERS-UPLC-MS/MS method to determine three kinds of ochratoxins in coffeeGAO Yunkai0CHEN Xiaomei1CHEN Chunqian2ZHOU Lingyu3DENG Yinglin4YIN Qingchun5 Hainan Institute for Food Control, Key Laboratory of Tropical Fruits and Vegetables Quality Safety for State Market Regulation, Haikou, Hainan 570100 , China Hainan Institute for Food Control, Key Laboratory of Tropical Fruits and Vegetables Quality Safety for State Market Regulation, Haikou, Hainan 570100 , China Hainan Institute for Food Control, Key Laboratory of Tropical Fruits and Vegetables Quality Safety for State Market Regulation, Haikou, Hainan 570100 , China Hainan Institute for Food Control, Key Laboratory of Tropical Fruits and Vegetables Quality Safety for State Market Regulation, Haikou, Hainan 570100 , China Hainan Institute for Food Control, Key Laboratory of Tropical Fruits and Vegetables Quality Safety for State Market Regulation, Haikou, Hainan 570100 , China Hainan Institute for Food Control, Key Laboratory of Tropical Fruits and Vegetables Quality Safety for State Market Regulation, Haikou, Hainan 570100 , China <b>Objective：</b> A new method was developed for the simultaneous determination of three kinds of ochratoxins in coffee by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) combined with QuEChERS pretreatment. <b>Methods：</b> Samples were extracted by acetonitri-water-formic acid solution (55∶40∶5) with ultrasonic, salted out with a QuEChERS salt pocket, and then purified with a (ZanChERS-Myco17) column. The chromatographic separation was performed on an ACQUITY UPLC BEH C<sub>18 </sub>(1.7 μm, 2.1 mm×100 mm) column by gradient elution using a mobile phase comprising 0.2% formic acid aqueous solution and acetonitrile. In the positive ion mode of electric spray, the samples detected were used by multiple reaction monitoring (MRM) modes with internal standard method. <b>Results：</b> Three target substances had a good linear relationship in the range of 0.1~20.0 ng/mL, and the correlation coefficient was greater than 0.999 46. The detection limit and quantitative limit were 0.1~0.2 μg/kg and 0.2~0.7 μg/kg, respectively. Average recoveries at three spiked levels in the coffee matrix were 79.0%~103.3% with a relative standard deviation of 1.5%~7.6%. <b>Conclusion：</b> This method has the advantages of simple sample pretreatment, good reproducibility and high efficiency, and is suitable for the high-throughput detection of multiple ochratoxins in different coffee samples.http://www.ifoodmm.com/spyjxen/article/abstract/20231115 coffee ochratoxins quechers uplc-ms/ms |
| spellingShingle | GAO Yunkai CHEN Xiaomei CHEN Chunqian ZHOU Lingyu DENG Yinglin YIN Qingchun Establishment of QuEChERS-UPLC-MS/MS method to determine three kinds of ochratoxins in coffee Shipin yu jixie coffee ochratoxins quechers uplc-ms/ms |
| title | Establishment of QuEChERS-UPLC-MS/MS method to determine three kinds of ochratoxins in coffee |
| title_full | Establishment of QuEChERS-UPLC-MS/MS method to determine three kinds of ochratoxins in coffee |
| title_fullStr | Establishment of QuEChERS-UPLC-MS/MS method to determine three kinds of ochratoxins in coffee |
| title_full_unstemmed | Establishment of QuEChERS-UPLC-MS/MS method to determine three kinds of ochratoxins in coffee |
| title_short | Establishment of QuEChERS-UPLC-MS/MS method to determine three kinds of ochratoxins in coffee |
| title_sort | establishment of quechers uplc ms ms method to determine three kinds of ochratoxins in coffee |
| topic | coffee ochratoxins quechers uplc-ms/ms |
| url | http://www.ifoodmm.com/spyjxen/article/abstract/20231115 |
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