Boosting industrial bio-denitrification through gene activation: the micro-nano-MoS2 enhanced bioprocess
Abstract High levels of nitrates and nitrites not only threaten aquatic ecosystems and drinking water safety but also impair the biodegradation efficiency of industrial wastewater. In this study, micro-nano-MoS2-1013 (0.04 g/L) enhanced denitrification by 56.9% and 29.6% in steel pickling and meat p...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-07-01
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| Series: | npj Clean Water |
| Online Access: | https://doi.org/10.1038/s41545-025-00494-y |
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| author | Zhaozhou Peng Jiangyuan Sun Panpan Li Junzhang Li Chen Shen Xia Tian Jing Lian Phathutshedzo Khangale Xiangjing Zhang Tonderayi Matambo Diane Hildebrandt Xinying Liu Shouxin Liu |
| author_facet | Zhaozhou Peng Jiangyuan Sun Panpan Li Junzhang Li Chen Shen Xia Tian Jing Lian Phathutshedzo Khangale Xiangjing Zhang Tonderayi Matambo Diane Hildebrandt Xinying Liu Shouxin Liu |
| author_sort | Zhaozhou Peng |
| collection | DOAJ |
| description | Abstract High levels of nitrates and nitrites not only threaten aquatic ecosystems and drinking water safety but also impair the biodegradation efficiency of industrial wastewater. In this study, micro-nano-MoS2-1013 (0.04 g/L) enhanced denitrification by 56.9% and 29.6% in steel pickling and meat processing wastewaters, respectively, and improved chemical oxygen demand (COD) removal by 136.7% in refinery cooling wastewater under continuous-flow conditions using a 3.5 L upflow anaerobic sludge blanket (UASB) reactor. The catalytic effect of micro-nano-MoS2 on denitrification was achieved by stimulating an increase in the abundance of denitrification genes and the transcript levels of narL (93.82 times), narG (16.34 times), and nirK (12.27 times) within the bacterial cells, which led to an increase in the expression levels of denitrifying enzymes. These findings have significant implications for the design and optimization of biodegradation processes and bio-denitrification systems, particularly for the treatment of high-concentration nitrate wastewater. |
| format | Article |
| id | doaj-art-21669ec7e82e48e1a824fdfde9f885ed |
| institution | Kabale University |
| issn | 2059-7037 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Clean Water |
| spelling | doaj-art-21669ec7e82e48e1a824fdfde9f885ed2025-08-20T03:45:40ZengNature Portfolionpj Clean Water2059-70372025-07-01811910.1038/s41545-025-00494-yBoosting industrial bio-denitrification through gene activation: the micro-nano-MoS2 enhanced bioprocessZhaozhou Peng0Jiangyuan Sun1Panpan Li2Junzhang Li3Chen Shen4Xia Tian5Jing Lian6Phathutshedzo Khangale7Xiangjing Zhang8Tonderayi Matambo9Diane Hildebrandt10Xinying Liu11Shouxin Liu12Hebei University of Science and TechnologyHebei University of Science and TechnologyHebei University of Science and TechnologyHebei University of Science and TechnologyHebei University of Science and TechnologyHebei University of Science and TechnologyHebei University of Science and TechnologyUniversity of South AfricaHebei University of Science and TechnologyUniversity of South AfricaDepartment of Chemical and Biochemical Engineering, Rutgers UniversityUniversity of South AfricaHebei University of Science and TechnologyAbstract High levels of nitrates and nitrites not only threaten aquatic ecosystems and drinking water safety but also impair the biodegradation efficiency of industrial wastewater. In this study, micro-nano-MoS2-1013 (0.04 g/L) enhanced denitrification by 56.9% and 29.6% in steel pickling and meat processing wastewaters, respectively, and improved chemical oxygen demand (COD) removal by 136.7% in refinery cooling wastewater under continuous-flow conditions using a 3.5 L upflow anaerobic sludge blanket (UASB) reactor. The catalytic effect of micro-nano-MoS2 on denitrification was achieved by stimulating an increase in the abundance of denitrification genes and the transcript levels of narL (93.82 times), narG (16.34 times), and nirK (12.27 times) within the bacterial cells, which led to an increase in the expression levels of denitrifying enzymes. These findings have significant implications for the design and optimization of biodegradation processes and bio-denitrification systems, particularly for the treatment of high-concentration nitrate wastewater.https://doi.org/10.1038/s41545-025-00494-y |
| spellingShingle | Zhaozhou Peng Jiangyuan Sun Panpan Li Junzhang Li Chen Shen Xia Tian Jing Lian Phathutshedzo Khangale Xiangjing Zhang Tonderayi Matambo Diane Hildebrandt Xinying Liu Shouxin Liu Boosting industrial bio-denitrification through gene activation: the micro-nano-MoS2 enhanced bioprocess npj Clean Water |
| title | Boosting industrial bio-denitrification through gene activation: the micro-nano-MoS2 enhanced bioprocess |
| title_full | Boosting industrial bio-denitrification through gene activation: the micro-nano-MoS2 enhanced bioprocess |
| title_fullStr | Boosting industrial bio-denitrification through gene activation: the micro-nano-MoS2 enhanced bioprocess |
| title_full_unstemmed | Boosting industrial bio-denitrification through gene activation: the micro-nano-MoS2 enhanced bioprocess |
| title_short | Boosting industrial bio-denitrification through gene activation: the micro-nano-MoS2 enhanced bioprocess |
| title_sort | boosting industrial bio denitrification through gene activation the micro nano mos2 enhanced bioprocess |
| url | https://doi.org/10.1038/s41545-025-00494-y |
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