Moesin integrates cortical and lamellar actin networks during Drosophila macrophage migration
Abstract Cells are thought to adopt mechanistically distinct migration modes depending on cell-type and environmental factors. These modes are assumed to be driven by mutually exclusive actin cytoskeletal organizations, which are either lamellar (flat, branched network) or cortical (crosslinked to t...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-02-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-55510-5 |
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| _version_ | 1823861785496649728 |
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| author | Besaiz J. Sánchez-Sánchez Stefania Marcotti David Salvador-Garcia María-del-Carmen Díaz-de-la-Loza Mubarik Burki Andrew J. Davidson Will Wood Brian M. Stramer |
| author_facet | Besaiz J. Sánchez-Sánchez Stefania Marcotti David Salvador-Garcia María-del-Carmen Díaz-de-la-Loza Mubarik Burki Andrew J. Davidson Will Wood Brian M. Stramer |
| author_sort | Besaiz J. Sánchez-Sánchez |
| collection | DOAJ |
| description | Abstract Cells are thought to adopt mechanistically distinct migration modes depending on cell-type and environmental factors. These modes are assumed to be driven by mutually exclusive actin cytoskeletal organizations, which are either lamellar (flat, branched network) or cortical (crosslinked to the plasma membrane). Here we exploit Drosophila macrophage (hemocyte) developmental dispersal to reveal that these cells maintain both a lamellar actin network at their cell front and a cortical actin network at the rear. Loss of classical actin cortex regulators, such as Moesin, perturb hemocyte morphology and cell migration. Furthermore, cortical and lamellipodial actin networks are interregulated. Upon phosphorylation and binding to the plasma membrane, Moesin is advected to the rear by lamellar actin flow. Simultaneously, the cortical actin network feeds back on the lamella to help regulate actin flow speed and leading-edge dynamics. These data reveal that hemocyte motility requires both lamellipodial and cortical actin architectures in homeostatic equilibrium. |
| format | Article |
| id | doaj-art-215c1f5a4ddc4b09bd73067d968e014d |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-215c1f5a4ddc4b09bd73067d968e014d2025-02-09T12:45:51ZengNature PortfolioNature Communications2041-17232025-02-0116111810.1038/s41467-024-55510-5Moesin integrates cortical and lamellar actin networks during Drosophila macrophage migrationBesaiz J. Sánchez-Sánchez0Stefania Marcotti1David Salvador-Garcia2María-del-Carmen Díaz-de-la-Loza3Mubarik Burki4Andrew J. Davidson5Will Wood6Brian M. Stramer7Randall Centre for Cell and Molecular Biophysics, King’s College LondonRandall Centre for Cell and Molecular Biophysics, King’s College LondonRandall Centre for Cell and Molecular Biophysics, King’s College LondonRandall Centre for Cell and Molecular Biophysics, King’s College LondonRandall Centre for Cell and Molecular Biophysics, King’s College LondonWolfson Wohl Cancer Research Centre, School of Cancer Sciences, University of Glasgow, Garscube Estate, Switchback Road, BearsdenCentre for Inflammation Research, Institute for Regeneration and Repair, University of Edinburgh, 5 Little France Drive, Edinburgh BioquarterRandall Centre for Cell and Molecular Biophysics, King’s College LondonAbstract Cells are thought to adopt mechanistically distinct migration modes depending on cell-type and environmental factors. These modes are assumed to be driven by mutually exclusive actin cytoskeletal organizations, which are either lamellar (flat, branched network) or cortical (crosslinked to the plasma membrane). Here we exploit Drosophila macrophage (hemocyte) developmental dispersal to reveal that these cells maintain both a lamellar actin network at their cell front and a cortical actin network at the rear. Loss of classical actin cortex regulators, such as Moesin, perturb hemocyte morphology and cell migration. Furthermore, cortical and lamellipodial actin networks are interregulated. Upon phosphorylation and binding to the plasma membrane, Moesin is advected to the rear by lamellar actin flow. Simultaneously, the cortical actin network feeds back on the lamella to help regulate actin flow speed and leading-edge dynamics. These data reveal that hemocyte motility requires both lamellipodial and cortical actin architectures in homeostatic equilibrium.https://doi.org/10.1038/s41467-024-55510-5 |
| spellingShingle | Besaiz J. Sánchez-Sánchez Stefania Marcotti David Salvador-Garcia María-del-Carmen Díaz-de-la-Loza Mubarik Burki Andrew J. Davidson Will Wood Brian M. Stramer Moesin integrates cortical and lamellar actin networks during Drosophila macrophage migration Nature Communications |
| title | Moesin integrates cortical and lamellar actin networks during Drosophila macrophage migration |
| title_full | Moesin integrates cortical and lamellar actin networks during Drosophila macrophage migration |
| title_fullStr | Moesin integrates cortical and lamellar actin networks during Drosophila macrophage migration |
| title_full_unstemmed | Moesin integrates cortical and lamellar actin networks during Drosophila macrophage migration |
| title_short | Moesin integrates cortical and lamellar actin networks during Drosophila macrophage migration |
| title_sort | moesin integrates cortical and lamellar actin networks during drosophila macrophage migration |
| url | https://doi.org/10.1038/s41467-024-55510-5 |
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