Detection of Dysplastic Intestinal Adenomas Using a Fluorescent Folate Imaging Probe
Macrophages have long been recognized as a prominent component of tumors. Activated macrophages overexpress folate receptors and we used this phenomenon to image inflammatory reactions in colon dysplasia using a fluorescent folate probe (FFP). APC Δ468 mice injected with FFP showed fluorescent adeno...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2005-01-01
|
| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.1162/15353500200504199 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850081453159743488 |
|---|---|
| author | Wei-Tsung Chen Khashayarsha Khazaie Guoying Zhang Ralph Weissleder Ching-Hsuan Tung |
| author_facet | Wei-Tsung Chen Khashayarsha Khazaie Guoying Zhang Ralph Weissleder Ching-Hsuan Tung |
| author_sort | Wei-Tsung Chen |
| collection | DOAJ |
| description | Macrophages have long been recognized as a prominent component of tumors. Activated macrophages overexpress folate receptors and we used this phenomenon to image inflammatory reactions in colon dysplasia using a fluorescent folate probe (FFP). APC Δ468 mice injected with FFP showed fluorescent adenomas (target-to-background ratio, adenoma vs. adjacent normal mucosa, of 2.46 ± 0.41), significantly higher ( p < .001) than adenomas in animals injected with a non-folate-containing control probe. Fluorescence-activated cell-sorting analysis revealed a 3-fold higher content of Mac1-positive cells in colonic adenomas compared with normal adjacent mucosa (6.8% vs. 2.2%), and confirmed the source of FFP-positive cells to be primarily an F4/80-positive macrophage subpopulation. Taken together, these results indicate that FFP potentially can be used to image dysplastic intestinal adenomas in vivo. |
| format | Article |
| id | doaj-art-2156c8ac2fbd4c318190cfcdbcfbfae7 |
| institution | DOAJ |
| issn | 1536-0121 |
| language | English |
| publishDate | 2005-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-2156c8ac2fbd4c318190cfcdbcfbfae72025-08-20T02:44:43ZengSAGE PublishingMolecular Imaging1536-01212005-01-01410.1162/1535350020050419910.1162_15353500200504199Detection of Dysplastic Intestinal Adenomas Using a Fluorescent Folate Imaging ProbeWei-Tsung Chen0Khashayarsha Khazaie1Guoying Zhang2Ralph Weissleder3Ching-Hsuan TungHarvard Medical School, CharlestownHarvard Medical School, BostonHarvard Medical School, BostonHarvard Medical School, CharlestownMacrophages have long been recognized as a prominent component of tumors. Activated macrophages overexpress folate receptors and we used this phenomenon to image inflammatory reactions in colon dysplasia using a fluorescent folate probe (FFP). APC Δ468 mice injected with FFP showed fluorescent adenomas (target-to-background ratio, adenoma vs. adjacent normal mucosa, of 2.46 ± 0.41), significantly higher ( p < .001) than adenomas in animals injected with a non-folate-containing control probe. Fluorescence-activated cell-sorting analysis revealed a 3-fold higher content of Mac1-positive cells in colonic adenomas compared with normal adjacent mucosa (6.8% vs. 2.2%), and confirmed the source of FFP-positive cells to be primarily an F4/80-positive macrophage subpopulation. Taken together, these results indicate that FFP potentially can be used to image dysplastic intestinal adenomas in vivo.https://doi.org/10.1162/15353500200504199 |
| spellingShingle | Wei-Tsung Chen Khashayarsha Khazaie Guoying Zhang Ralph Weissleder Ching-Hsuan Tung Detection of Dysplastic Intestinal Adenomas Using a Fluorescent Folate Imaging Probe Molecular Imaging |
| title | Detection of Dysplastic Intestinal Adenomas Using a Fluorescent Folate Imaging Probe |
| title_full | Detection of Dysplastic Intestinal Adenomas Using a Fluorescent Folate Imaging Probe |
| title_fullStr | Detection of Dysplastic Intestinal Adenomas Using a Fluorescent Folate Imaging Probe |
| title_full_unstemmed | Detection of Dysplastic Intestinal Adenomas Using a Fluorescent Folate Imaging Probe |
| title_short | Detection of Dysplastic Intestinal Adenomas Using a Fluorescent Folate Imaging Probe |
| title_sort | detection of dysplastic intestinal adenomas using a fluorescent folate imaging probe |
| url | https://doi.org/10.1162/15353500200504199 |
| work_keys_str_mv | AT weitsungchen detectionofdysplasticintestinaladenomasusingafluorescentfolateimagingprobe AT khashayarshakhazaie detectionofdysplasticintestinaladenomasusingafluorescentfolateimagingprobe AT guoyingzhang detectionofdysplasticintestinaladenomasusingafluorescentfolateimagingprobe AT ralphweissleder detectionofdysplasticintestinaladenomasusingafluorescentfolateimagingprobe AT chinghsuantung detectionofdysplasticintestinaladenomasusingafluorescentfolateimagingprobe |