Characterization and application of fluorescent hydrogel films with superior mechanical properties in detecting iron(Ⅲ) ions and ferroptosis in oral cancer
A one-step hydrothermal method was applied to prepare carbon dots (CDs) with superior fluorescence properties using chitosan as a carbon source. The as-prepared carbon dots were then grafted onto a sodium alginate-gelatin hydrogel film to form a fluorescent hydrogel film (FHGF), emitting at 450 nm u...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1526877/full |
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| author | Jinxi Wen Jian Wang Siqi Wang Xingping Zhou You Fu |
| author_facet | Jinxi Wen Jian Wang Siqi Wang Xingping Zhou You Fu |
| author_sort | Jinxi Wen |
| collection | DOAJ |
| description | A one-step hydrothermal method was applied to prepare carbon dots (CDs) with superior fluorescence properties using chitosan as a carbon source. The as-prepared carbon dots were then grafted onto a sodium alginate-gelatin hydrogel film to form a fluorescent hydrogel film (FHGF), emitting at 450 nm under excitation of 350 nm light. In comparison to the CDs, the fluorescence intensity of this film was maintained over 90.0% and the luminescence position remained basically unchanged, caused by the unchanged surface light-emitting structure of the CDs, due to the existence of electrostatic repulsion between the CDs and the hydrogel. Moreover, the tensile-stress of the fluorescent film with 1.0 wt.% of the CDs was increased by 200% to 10.3 Mpa, and the strain was increased from 117% to 153%. The above experimental results are attributed to the hydrogen bonding between the CDs and the sodium alginate-gelatin hydrogel from analyses of the FT-IR spectra. Interestingly, Fe3+ exerted a great quenching effect on this fluorescent film in the concentration range of 0–1.8 μM. The film can be basically used recyclically to detect Fe3+ in solution with a detection limit as low as 0.043 μM. In a word, this work demonstrated an enormous potential of carbon dots in fabricating mechanical and fluorescent properties of the hydrogel and proposed a new detection platform for Fe3+. In view of the promising Fe3+ detection capacity, this hydrogel film can also be applied in oral bacteria surveillance and semi-quantification of ferroptosis in oral cancer. |
| format | Article |
| id | doaj-art-2150c8993c7a41f9ba96c118fead242c |
| institution | Kabale University |
| issn | 2296-4185 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-2150c8993c7a41f9ba96c118fead242c2025-01-14T06:10:23ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-01-011210.3389/fbioe.2024.15268771526877Characterization and application of fluorescent hydrogel films with superior mechanical properties in detecting iron(Ⅲ) ions and ferroptosis in oral cancerJinxi Wen0Jian Wang1Siqi Wang2Xingping Zhou3You Fu4College of Biological Science and Medical Engineering, Donghua University, Shanghai, ChinaDepartment of General Dentistry, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, Research Unit of Oral and Maxillofacial Regenerative Medicine, Chinese Academy of Medical Sciences, Shanghai, ChinaCollege of Biological Science and Medical Engineering, Donghua University, Shanghai, ChinaCollege of Biological Science and Medical Engineering, Donghua University, Shanghai, ChinaDepartment of Orthodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, Research Unit of Oral and Maxillofacial Regenerative Medicine, Chinese Academy of Medical Sciences, Shanghai, ChinaA one-step hydrothermal method was applied to prepare carbon dots (CDs) with superior fluorescence properties using chitosan as a carbon source. The as-prepared carbon dots were then grafted onto a sodium alginate-gelatin hydrogel film to form a fluorescent hydrogel film (FHGF), emitting at 450 nm under excitation of 350 nm light. In comparison to the CDs, the fluorescence intensity of this film was maintained over 90.0% and the luminescence position remained basically unchanged, caused by the unchanged surface light-emitting structure of the CDs, due to the existence of electrostatic repulsion between the CDs and the hydrogel. Moreover, the tensile-stress of the fluorescent film with 1.0 wt.% of the CDs was increased by 200% to 10.3 Mpa, and the strain was increased from 117% to 153%. The above experimental results are attributed to the hydrogen bonding between the CDs and the sodium alginate-gelatin hydrogel from analyses of the FT-IR spectra. Interestingly, Fe3+ exerted a great quenching effect on this fluorescent film in the concentration range of 0–1.8 μM. The film can be basically used recyclically to detect Fe3+ in solution with a detection limit as low as 0.043 μM. In a word, this work demonstrated an enormous potential of carbon dots in fabricating mechanical and fluorescent properties of the hydrogel and proposed a new detection platform for Fe3+. In view of the promising Fe3+ detection capacity, this hydrogel film can also be applied in oral bacteria surveillance and semi-quantification of ferroptosis in oral cancer.https://www.frontiersin.org/articles/10.3389/fbioe.2024.1526877/fullcarbon dotsfluorescent hydrogel filmFe3+mechanical propertiesferroptosis |
| spellingShingle | Jinxi Wen Jian Wang Siqi Wang Xingping Zhou You Fu Characterization and application of fluorescent hydrogel films with superior mechanical properties in detecting iron(Ⅲ) ions and ferroptosis in oral cancer Frontiers in Bioengineering and Biotechnology carbon dots fluorescent hydrogel film Fe3+ mechanical properties ferroptosis |
| title | Characterization and application of fluorescent hydrogel films with superior mechanical properties in detecting iron(Ⅲ) ions and ferroptosis in oral cancer |
| title_full | Characterization and application of fluorescent hydrogel films with superior mechanical properties in detecting iron(Ⅲ) ions and ferroptosis in oral cancer |
| title_fullStr | Characterization and application of fluorescent hydrogel films with superior mechanical properties in detecting iron(Ⅲ) ions and ferroptosis in oral cancer |
| title_full_unstemmed | Characterization and application of fluorescent hydrogel films with superior mechanical properties in detecting iron(Ⅲ) ions and ferroptosis in oral cancer |
| title_short | Characterization and application of fluorescent hydrogel films with superior mechanical properties in detecting iron(Ⅲ) ions and ferroptosis in oral cancer |
| title_sort | characterization and application of fluorescent hydrogel films with superior mechanical properties in detecting iron iii ions and ferroptosis in oral cancer |
| topic | carbon dots fluorescent hydrogel film Fe3+ mechanical properties ferroptosis |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1526877/full |
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