Listeria monocytogenes membrane vesicles disrupting intestinal epithelial barrier function via modulating macrophage inflammatory responses
Abstract Bacterial extracellular vesicles (BEVs) are actively secreted nanostructures that play a unique role in the pathogenesis of bacterial infections. In this study, we established an intestinal epithelial cell (IEC)/macrophage coculture system and found that Listeria monocytogenes (LM) membrane...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley-VCH
2025-05-01
|
| Series: | Interdisciplinary Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/INMD.20240066 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849434392197332992 |
|---|---|
| author | Yao Lei Kehan Chen Mingyuan Tang Qiuyang Zhang Jie Yu Yunwen Zhang Tian Tang Chuan Wang |
| author_facet | Yao Lei Kehan Chen Mingyuan Tang Qiuyang Zhang Jie Yu Yunwen Zhang Tian Tang Chuan Wang |
| author_sort | Yao Lei |
| collection | DOAJ |
| description | Abstract Bacterial extracellular vesicles (BEVs) are actively secreted nanostructures that play a unique role in the pathogenesis of bacterial infections. In this study, we established an intestinal epithelial cell (IEC)/macrophage coculture system and found that Listeria monocytogenes (LM) membrane vesicles (MVs) acted on macrophages and caused intestinal barrier disruption. Further studies revealed that LM MVs not only induced the M1‐type polarization in macrophages through the MAPK signaling pathway but also activated AIM2 and NLRP3 inflammasomes in macrophages to induce pyroptosis. The inflammatory factors IFN‐α and TNF‐α released by macrophages can bind to IFNAR and TNFR in IECs and activate the JAK‐STAT3 signaling pathway, disrupting the intestinal barrier. Our study illustrates the role and mechanism of LM MVs in disrupting the intestinal barrier by regulating the inflammatory response of macrophages, which helps understand the function of LM MVs, explains the mechanism of LM‐intestinal infection, and may provide new targets for the treatment of intestinal invasive infections with LM. |
| format | Article |
| id | doaj-art-2129a1943ef44f148887fcd18958d336 |
| institution | Kabale University |
| issn | 2832-6245 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Interdisciplinary Medicine |
| spelling | doaj-art-2129a1943ef44f148887fcd18958d3362025-08-20T03:26:39ZengWiley-VCHInterdisciplinary Medicine2832-62452025-05-0133n/an/a10.1002/INMD.20240066Listeria monocytogenes membrane vesicles disrupting intestinal epithelial barrier function via modulating macrophage inflammatory responsesYao Lei0Kehan Chen1Mingyuan Tang2Qiuyang Zhang3Jie Yu4Yunwen Zhang5Tian Tang6Chuan Wang7West China School of Public Health and West China Fourth Hospital Sichuan University Chengdu ChinaWest China School of Public Health and West China Fourth Hospital Sichuan University Chengdu ChinaWest China School of Public Health and West China Fourth Hospital Sichuan University Chengdu ChinaWest China School of Public Health and West China Fourth Hospital Sichuan University Chengdu ChinaWest China School of Public Health and West China Fourth Hospital Sichuan University Chengdu ChinaWest China School of Public Health and West China Fourth Hospital Sichuan University Chengdu ChinaWest China School of Public Health and West China Fourth Hospital Sichuan University Chengdu ChinaWest China School of Public Health and West China Fourth Hospital Sichuan University Chengdu ChinaAbstract Bacterial extracellular vesicles (BEVs) are actively secreted nanostructures that play a unique role in the pathogenesis of bacterial infections. In this study, we established an intestinal epithelial cell (IEC)/macrophage coculture system and found that Listeria monocytogenes (LM) membrane vesicles (MVs) acted on macrophages and caused intestinal barrier disruption. Further studies revealed that LM MVs not only induced the M1‐type polarization in macrophages through the MAPK signaling pathway but also activated AIM2 and NLRP3 inflammasomes in macrophages to induce pyroptosis. The inflammatory factors IFN‐α and TNF‐α released by macrophages can bind to IFNAR and TNFR in IECs and activate the JAK‐STAT3 signaling pathway, disrupting the intestinal barrier. Our study illustrates the role and mechanism of LM MVs in disrupting the intestinal barrier by regulating the inflammatory response of macrophages, which helps understand the function of LM MVs, explains the mechanism of LM‐intestinal infection, and may provide new targets for the treatment of intestinal invasive infections with LM.https://doi.org/10.1002/INMD.20240066inflammatory responseintestinal epithelial cellsListeria monocytogenesmacrophagesmembrane vesicles |
| spellingShingle | Yao Lei Kehan Chen Mingyuan Tang Qiuyang Zhang Jie Yu Yunwen Zhang Tian Tang Chuan Wang Listeria monocytogenes membrane vesicles disrupting intestinal epithelial barrier function via modulating macrophage inflammatory responses Interdisciplinary Medicine inflammatory response intestinal epithelial cells Listeria monocytogenes macrophages membrane vesicles |
| title | Listeria monocytogenes membrane vesicles disrupting intestinal epithelial barrier function via modulating macrophage inflammatory responses |
| title_full | Listeria monocytogenes membrane vesicles disrupting intestinal epithelial barrier function via modulating macrophage inflammatory responses |
| title_fullStr | Listeria monocytogenes membrane vesicles disrupting intestinal epithelial barrier function via modulating macrophage inflammatory responses |
| title_full_unstemmed | Listeria monocytogenes membrane vesicles disrupting intestinal epithelial barrier function via modulating macrophage inflammatory responses |
| title_short | Listeria monocytogenes membrane vesicles disrupting intestinal epithelial barrier function via modulating macrophage inflammatory responses |
| title_sort | listeria monocytogenes membrane vesicles disrupting intestinal epithelial barrier function via modulating macrophage inflammatory responses |
| topic | inflammatory response intestinal epithelial cells Listeria monocytogenes macrophages membrane vesicles |
| url | https://doi.org/10.1002/INMD.20240066 |
| work_keys_str_mv | AT yaolei listeriamonocytogenesmembranevesiclesdisruptingintestinalepithelialbarrierfunctionviamodulatingmacrophageinflammatoryresponses AT kehanchen listeriamonocytogenesmembranevesiclesdisruptingintestinalepithelialbarrierfunctionviamodulatingmacrophageinflammatoryresponses AT mingyuantang listeriamonocytogenesmembranevesiclesdisruptingintestinalepithelialbarrierfunctionviamodulatingmacrophageinflammatoryresponses AT qiuyangzhang listeriamonocytogenesmembranevesiclesdisruptingintestinalepithelialbarrierfunctionviamodulatingmacrophageinflammatoryresponses AT jieyu listeriamonocytogenesmembranevesiclesdisruptingintestinalepithelialbarrierfunctionviamodulatingmacrophageinflammatoryresponses AT yunwenzhang listeriamonocytogenesmembranevesiclesdisruptingintestinalepithelialbarrierfunctionviamodulatingmacrophageinflammatoryresponses AT tiantang listeriamonocytogenesmembranevesiclesdisruptingintestinalepithelialbarrierfunctionviamodulatingmacrophageinflammatoryresponses AT chuanwang listeriamonocytogenesmembranevesiclesdisruptingintestinalepithelialbarrierfunctionviamodulatingmacrophageinflammatoryresponses |