Effect of Cold Storage Time (4°C) on Malondialdehyde (MDA) Level, Motility and Viability Spermatozoa of Cyprinus carpio L. Punten Strain
The aim of this research is to know the level of MDA, motility, and viability spermatozoa of C. carpio L. in cold storage time at 4°C. This study used treatment extenders that were NaCl 0.9% as control, diluent of egg yolk with concentrate 0%, 5%, 10%, 15%, and storage in 0 h, 24 h, 48 h, 72 h, and...
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University of Brawijaya
2020-06-01
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| Series: | Journal of Experimental Life Science |
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| Online Access: | https://jels.ub.ac.id/index.php/jels/article/view/411 |
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| author | Rosyi Wirastuti Agung Pramana Warih Marhendra Jantje Wiliem Souhaly Sri Rahayu |
| author_facet | Rosyi Wirastuti Agung Pramana Warih Marhendra Jantje Wiliem Souhaly Sri Rahayu |
| author_sort | Rosyi Wirastuti |
| collection | DOAJ |
| description | The aim of this research is to know the level of MDA, motility, and viability spermatozoa of C. carpio L. in cold storage time at 4°C. This study used treatment extenders that were NaCl 0.9% as control, diluent of egg yolk with concentrate 0%, 5%, 10%, 15%, and storage in 0 h, 24 h, 48 h, 72 h, and 96 h at 4°C. The data were analyzed using ANOVA (P<0.05). The results showed that the quality (motility and viability) of spermatozoa C. carpio L. punten strain in cold storage could be maintained at 96 h. The optimum storage for motility was found in diluent of egg yolk concentrate 5% at 48 h. MDA levels of semen from C. carpio L. Punten strains after being stored at cold temperatures increased at 0 h to 24 h, then decreased after 48 h of storage and increase at 72 h - 96 h stored. Egg yolk concentration and storage time had a significant effect on MDA levels. There is a negative correlation between MDA levels and motility. There was no correlation between MDA levels and the viability of spermatozoa C. carpio L. punten strain. |
| format | Article |
| id | doaj-art-210da704b6924444a728e1d64d5a63f9 |
| institution | OA Journals |
| issn | 2087-2852 2338-1655 |
| language | English |
| publishDate | 2020-06-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Experimental Life Science |
| spelling | doaj-art-210da704b6924444a728e1d64d5a63f92025-08-20T02:30:50ZengUniversity of BrawijayaJournal of Experimental Life Science2087-28522338-16552020-06-01102127131https://doi.org/10.21776/ub.jels.2020.010.02.08Effect of Cold Storage Time (4°C) on Malondialdehyde (MDA) Level, Motility and Viability Spermatozoa of Cyprinus carpio L. Punten StrainRosyi Wirastuti0Agung Pramana Warih Marhendra1Jantje Wiliem Souhaly2Sri Rahayu3Universitas BrawijayaUniversitas BrawijayaUniversitas BrawijayaUniversitas BrawijayaThe aim of this research is to know the level of MDA, motility, and viability spermatozoa of C. carpio L. in cold storage time at 4°C. This study used treatment extenders that were NaCl 0.9% as control, diluent of egg yolk with concentrate 0%, 5%, 10%, 15%, and storage in 0 h, 24 h, 48 h, 72 h, and 96 h at 4°C. The data were analyzed using ANOVA (P<0.05). The results showed that the quality (motility and viability) of spermatozoa C. carpio L. punten strain in cold storage could be maintained at 96 h. The optimum storage for motility was found in diluent of egg yolk concentrate 5% at 48 h. MDA levels of semen from C. carpio L. Punten strains after being stored at cold temperatures increased at 0 h to 24 h, then decreased after 48 h of storage and increase at 72 h - 96 h stored. Egg yolk concentration and storage time had a significant effect on MDA levels. There is a negative correlation between MDA levels and motility. There was no correlation between MDA levels and the viability of spermatozoa C. carpio L. punten strain.https://jels.ub.ac.id/index.php/jels/article/view/411c. carpio legg yolkmalondialdehydemotiliyviability |
| spellingShingle | Rosyi Wirastuti Agung Pramana Warih Marhendra Jantje Wiliem Souhaly Sri Rahayu Effect of Cold Storage Time (4°C) on Malondialdehyde (MDA) Level, Motility and Viability Spermatozoa of Cyprinus carpio L. Punten Strain Journal of Experimental Life Science c. carpio l egg yolk malondialdehyde motiliy viability |
| title | Effect of Cold Storage Time (4°C) on Malondialdehyde (MDA) Level, Motility and Viability Spermatozoa of Cyprinus carpio L. Punten Strain |
| title_full | Effect of Cold Storage Time (4°C) on Malondialdehyde (MDA) Level, Motility and Viability Spermatozoa of Cyprinus carpio L. Punten Strain |
| title_fullStr | Effect of Cold Storage Time (4°C) on Malondialdehyde (MDA) Level, Motility and Viability Spermatozoa of Cyprinus carpio L. Punten Strain |
| title_full_unstemmed | Effect of Cold Storage Time (4°C) on Malondialdehyde (MDA) Level, Motility and Viability Spermatozoa of Cyprinus carpio L. Punten Strain |
| title_short | Effect of Cold Storage Time (4°C) on Malondialdehyde (MDA) Level, Motility and Viability Spermatozoa of Cyprinus carpio L. Punten Strain |
| title_sort | effect of cold storage time 4°c on malondialdehyde mda level motility and viability spermatozoa of cyprinus carpio l punten strain |
| topic | c. carpio l egg yolk malondialdehyde motiliy viability |
| url | https://jels.ub.ac.id/index.php/jels/article/view/411 |
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