The longitudinal impact of low-dose morphine on diurnal cortisol profiles in people with chronic breathlessness and chronic obstructive pulmonary disease (COPD): an exploratory study

Abstract Introduction Stress activates the hypothalamic-pituitary-adrenal (HPA) axis of which cortisol is an end product. ‘Allostatic load’ is where systems including the HPA axis are exposed to high, cumulative, physiologic burdens (such as chronic breathlessness) leading to flatter diurnal cortiso...

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Main Authors: Diana H. Ferreira, Richella Ryan, Nina Smyth, Angela Clow, David C. Currow
Format: Article
Language:English
Published: BMC 2025-04-01
Series:Respiratory Research
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Online Access:https://doi.org/10.1186/s12931-025-03230-9
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author Diana H. Ferreira
Richella Ryan
Nina Smyth
Angela Clow
David C. Currow
author_facet Diana H. Ferreira
Richella Ryan
Nina Smyth
Angela Clow
David C. Currow
author_sort Diana H. Ferreira
collection DOAJ
description Abstract Introduction Stress activates the hypothalamic-pituitary-adrenal (HPA) axis of which cortisol is an end product. ‘Allostatic load’ is where systems including the HPA axis are exposed to high, cumulative, physiologic burdens (such as chronic breathlessness) leading to flatter diurnal cortisol slopes and poorer health outcomes. The aim of this hypothesis-generating study explored longitudinal changes in cortisol secretion and any associated changes in breathlessness after introducing regular, low dose morphine or placebo. Methods This was an optional, hypothesis-generating sub-study embedded in a multi-site, randomised, double-blind, placebo-controlled trial (RCT) of regular, low-dose morphine for chronic breathlessness and chronic obstructive pulmonary disease. In a blinded dose-increment algorithm by week three, doses were 0 mg-32 mg. Participants in the RCT could elect to continue in a six-month blinded extension. This sub-study excluded people who used non-inhaled corticosteroids in the previous month or were on subcutaneous insulin. Participants collected saliva for cortisol assays for two days at baseline, and ends of weeks 1, 3 and 12 at 3,6 and 12 h after waking, generating sufficient data to calculate diurnal cortisol slopes and areas under the curve (AUC). Samples were analysed using ELISA. Correlations between diurnal cortisol profiles (slope and AUC) and a range of measures were explored. Results Twenty mostly female former smokers were in this sub-study. At baseline and the end of week 1, one-way ANOVA between-group analyses showed no significant differences in the log-transformed cortisol slope or ln-AUC. There was a strong correlation between the age-adjusted Charlson Comorbidity Index (CCI) and ln-AUC (r=-0.70, p < 0.001) and moderate correlation with age (r=-0.43, p = 0.06). In the blinded extension study, there was a self-selecting blinded group (n = 7) all on active medication. Global impression of change (GIC) was highly correlated with the diurnal cortisol slope (rs = 0.98, p = 0.01), and with decrease in average breathlessness (r = 0.89, p = 0.04). Discussion This hypothesis-generating study did not show a relationship between the diurnal cortisol profile and morphine in people with chronic breathlessness and COPD. For the sub-group still on study at 12weeks, the cortisol curves became steeper as average breathlessness decreased and as global impression of change (GIC) improved, suggesting that reducing breathlessness may potentially positively impact the HPA axis in a sub-group of people. Trial registration Registration Number NCT02720822 date registered 28/03/2016.
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spelling doaj-art-2102baba2c554462bb7caa64a7795e7a2025-08-20T02:19:58ZengBMCRespiratory Research1465-993X2025-04-0126111110.1186/s12931-025-03230-9The longitudinal impact of low-dose morphine on diurnal cortisol profiles in people with chronic breathlessness and chronic obstructive pulmonary disease (COPD): an exploratory studyDiana H. Ferreira0Richella Ryan1Nina Smyth2Angela Clow3David C. Currow4Graduate School of Medicine, Faculty of Science, Medicine and Health, University of WollongongArthur Rank Hospice CharityUniversity of WestminsterUniversity of WestminsterFaculty of Health, University of Technology SydneyAbstract Introduction Stress activates the hypothalamic-pituitary-adrenal (HPA) axis of which cortisol is an end product. ‘Allostatic load’ is where systems including the HPA axis are exposed to high, cumulative, physiologic burdens (such as chronic breathlessness) leading to flatter diurnal cortisol slopes and poorer health outcomes. The aim of this hypothesis-generating study explored longitudinal changes in cortisol secretion and any associated changes in breathlessness after introducing regular, low dose morphine or placebo. Methods This was an optional, hypothesis-generating sub-study embedded in a multi-site, randomised, double-blind, placebo-controlled trial (RCT) of regular, low-dose morphine for chronic breathlessness and chronic obstructive pulmonary disease. In a blinded dose-increment algorithm by week three, doses were 0 mg-32 mg. Participants in the RCT could elect to continue in a six-month blinded extension. This sub-study excluded people who used non-inhaled corticosteroids in the previous month or were on subcutaneous insulin. Participants collected saliva for cortisol assays for two days at baseline, and ends of weeks 1, 3 and 12 at 3,6 and 12 h after waking, generating sufficient data to calculate diurnal cortisol slopes and areas under the curve (AUC). Samples were analysed using ELISA. Correlations between diurnal cortisol profiles (slope and AUC) and a range of measures were explored. Results Twenty mostly female former smokers were in this sub-study. At baseline and the end of week 1, one-way ANOVA between-group analyses showed no significant differences in the log-transformed cortisol slope or ln-AUC. There was a strong correlation between the age-adjusted Charlson Comorbidity Index (CCI) and ln-AUC (r=-0.70, p < 0.001) and moderate correlation with age (r=-0.43, p = 0.06). In the blinded extension study, there was a self-selecting blinded group (n = 7) all on active medication. Global impression of change (GIC) was highly correlated with the diurnal cortisol slope (rs = 0.98, p = 0.01), and with decrease in average breathlessness (r = 0.89, p = 0.04). Discussion This hypothesis-generating study did not show a relationship between the diurnal cortisol profile and morphine in people with chronic breathlessness and COPD. For the sub-group still on study at 12weeks, the cortisol curves became steeper as average breathlessness decreased and as global impression of change (GIC) improved, suggesting that reducing breathlessness may potentially positively impact the HPA axis in a sub-group of people. Trial registration Registration Number NCT02720822 date registered 28/03/2016.https://doi.org/10.1186/s12931-025-03230-9Chronic breathlessnessSustained-release morphineCortisolPalliative careSymptom control
spellingShingle Diana H. Ferreira
Richella Ryan
Nina Smyth
Angela Clow
David C. Currow
The longitudinal impact of low-dose morphine on diurnal cortisol profiles in people with chronic breathlessness and chronic obstructive pulmonary disease (COPD): an exploratory study
Respiratory Research
Chronic breathlessness
Sustained-release morphine
Cortisol
Palliative care
Symptom control
title The longitudinal impact of low-dose morphine on diurnal cortisol profiles in people with chronic breathlessness and chronic obstructive pulmonary disease (COPD): an exploratory study
title_full The longitudinal impact of low-dose morphine on diurnal cortisol profiles in people with chronic breathlessness and chronic obstructive pulmonary disease (COPD): an exploratory study
title_fullStr The longitudinal impact of low-dose morphine on diurnal cortisol profiles in people with chronic breathlessness and chronic obstructive pulmonary disease (COPD): an exploratory study
title_full_unstemmed The longitudinal impact of low-dose morphine on diurnal cortisol profiles in people with chronic breathlessness and chronic obstructive pulmonary disease (COPD): an exploratory study
title_short The longitudinal impact of low-dose morphine on diurnal cortisol profiles in people with chronic breathlessness and chronic obstructive pulmonary disease (COPD): an exploratory study
title_sort longitudinal impact of low dose morphine on diurnal cortisol profiles in people with chronic breathlessness and chronic obstructive pulmonary disease copd an exploratory study
topic Chronic breathlessness
Sustained-release morphine
Cortisol
Palliative care
Symptom control
url https://doi.org/10.1186/s12931-025-03230-9
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