Determination of triterpenoid saponin and polysaccharide content from in vitro cultures of Astragalus mongholicus Bunge
In this study, the efficient micropropagation protocol of Astragalus mongholicus Bunge was established and also triterpenoid saponin and polysaccharide content in ethanol, methanol and aqueous extracts of different samples were determined by using spectrophotometric methods to investigate whether th...
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Mongolian Academy of Sciences
2023-05-01
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| Series: | Proceedings of the Mongolian Academy of Sciences |
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| Online Access: | https://www.mongoliajol.info/index.php/PMAS/article/view/2804 |
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| author | Tsolmon Munkhbayar Batzaya Gachmaa Azzaya Jukov Oyunbileg Yungeree |
| author_facet | Tsolmon Munkhbayar Batzaya Gachmaa Azzaya Jukov Oyunbileg Yungeree |
| author_sort | Tsolmon Munkhbayar |
| collection | DOAJ |
| description | In this study, the efficient micropropagation protocol of Astragalus mongholicus Bunge was established and also triterpenoid saponin and polysaccharide content in ethanol, methanol and aqueous extracts of different samples were determined by using spectrophotometric methods to investigate whether the content of biologically active compounds depends on the stage of development of the plant during in vitro culture. The content of total saponins and polysaccharides in different cultures of A. mongholicus grown in vitro was higher (990 and 505 μg/ml) in ethanol extracted 14-day-old young shoot samples than in 28-day-old propagated shoot samples and rooted shoots. |
| format | Article |
| id | doaj-art-20f883d3054943bfba3fd3a45e2ee452 |
| institution | Kabale University |
| issn | 2310-4716 2312-2994 |
| language | English |
| publishDate | 2023-05-01 |
| publisher | Mongolian Academy of Sciences |
| record_format | Article |
| series | Proceedings of the Mongolian Academy of Sciences |
| spelling | doaj-art-20f883d3054943bfba3fd3a45e2ee4522025-08-20T03:25:27ZengMongolian Academy of SciencesProceedings of the Mongolian Academy of Sciences2310-47162312-29942023-05-0161510.5564/pmas.v63i01.28042755Determination of triterpenoid saponin and polysaccharide content from in vitro cultures of Astragalus mongholicus BungeTsolmon Munkhbayar0https://orcid.org/0000-0003-1543-8120Batzaya Gachmaa1https://orcid.org/0000-0001-7232-6542Azzaya Jukov2https://orcid.org/0000-0002-4221-9743Oyunbileg Yungeree3https://orcid.org/0000-0002-6400-0654Laboratory of Plant Biotechnology, Institute of Biology, Mongolian Academy of Sciences, Ulaanbaatar, MongoliaLaboratory of Plant Vegetation and Plant Economy, Botanic Garden and Research Institute, Mongolian Academy of Sciences, Ulaanbaatar, MongoliaLaboratory of Plant Vegetation and Plant Economy, Botanic Garden and Research Institute, Mongolian Academy of Sciences, Ulaanbaatar, MongoliaLaboratory of Plant Biotechnology, Institute of Biology, Mongolian Academy of Sciences, Ulaanbaatar, MongoliaIn this study, the efficient micropropagation protocol of Astragalus mongholicus Bunge was established and also triterpenoid saponin and polysaccharide content in ethanol, methanol and aqueous extracts of different samples were determined by using spectrophotometric methods to investigate whether the content of biologically active compounds depends on the stage of development of the plant during in vitro culture. The content of total saponins and polysaccharides in different cultures of A. mongholicus grown in vitro was higher (990 and 505 μg/ml) in ethanol extracted 14-day-old young shoot samples than in 28-day-old propagated shoot samples and rooted shoots.https://www.mongoliajol.info/index.php/PMAS/article/view/2804astragalus mongholicusin vitro culturessecondary metabolitetotal triterpenoid saponinpolysaccharide |
| spellingShingle | Tsolmon Munkhbayar Batzaya Gachmaa Azzaya Jukov Oyunbileg Yungeree Determination of triterpenoid saponin and polysaccharide content from in vitro cultures of Astragalus mongholicus Bunge Proceedings of the Mongolian Academy of Sciences astragalus mongholicus in vitro cultures secondary metabolite total triterpenoid saponin polysaccharide |
| title | Determination of triterpenoid saponin and polysaccharide content from in vitro cultures of Astragalus mongholicus Bunge |
| title_full | Determination of triterpenoid saponin and polysaccharide content from in vitro cultures of Astragalus mongholicus Bunge |
| title_fullStr | Determination of triterpenoid saponin and polysaccharide content from in vitro cultures of Astragalus mongholicus Bunge |
| title_full_unstemmed | Determination of triterpenoid saponin and polysaccharide content from in vitro cultures of Astragalus mongholicus Bunge |
| title_short | Determination of triterpenoid saponin and polysaccharide content from in vitro cultures of Astragalus mongholicus Bunge |
| title_sort | determination of triterpenoid saponin and polysaccharide content from in vitro cultures of astragalus mongholicus bunge |
| topic | astragalus mongholicus in vitro cultures secondary metabolite total triterpenoid saponin polysaccharide |
| url | https://www.mongoliajol.info/index.php/PMAS/article/view/2804 |
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