Insights into the Genetics Underlying the Resistance to Root-Knot Nematode Reproduction in the Common Bean Ouro Negro
Root-knot nematodes (RKNs, <i>Meloidogyne</i> spp.) have become the major yield-limiting biological factor in common bean production in many warmer-climate regions such as the south of Europe. Broadening the genetic base of resistance in elite common bean cultivars is the most effective...
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2025-04-01
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| author | Ana M. Pesqueira Ana M. González Teresa Barragán-Lozano María S. Arnedo Rafael Lozano Marta Santalla |
| author_facet | Ana M. Pesqueira Ana M. González Teresa Barragán-Lozano María S. Arnedo Rafael Lozano Marta Santalla |
| author_sort | Ana M. Pesqueira |
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| description | Root-knot nematodes (RKNs, <i>Meloidogyne</i> spp.) have become the major yield-limiting biological factor in common bean production in many warmer-climate regions such as the south of Europe. Broadening the genetic base of resistance in elite common bean cultivars is the most effective and environmentally friendly method for managing this disease. Toward this goal, F<sub>1</sub>, F<sub>2</sub>, and F<sub>3</sub> populations from crosses between susceptible snap beans (Helda and Perona) and the resistant Ouro Negro cultivar were phenotyped for <i>M. incognita</i> and <i>M. javanica</i>-induced root-galling (GI) and egg mass production (EM) in controlled growth chamber infection assays. F<sub>1</sub> progenies showed a susceptible response to both RKN isolates, with high GI and EM values, indicating a recessive inheritance of nematode resistance. The estimates for broad-sense heritability for GI and EM in the F<sub>2</sub> Helda × Ouro Negro population infected with <i>M. incognita</i> were 0.62 and 0.54, respectively. RKN resistance in Ouro Negro is largely controlled by partial to overdominant genetic effects and that susceptibility factor leads recessive resistance. The minimum number of genes involved in nematode resistance was estimated to be about two or three. In agreement, genetic analysis of F<sub>2</sub> segregating populations supported duplicate recessive epistasis as the inheritance pattern involved in the resistance provided by the Ouro Negro cultivar. Ouro Negro is an important resource for broadening RKN resistance in elite common bean cultivars. |
| format | Article |
| id | doaj-art-20e455bedb024a249803758e19e63ccc |
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| issn | 2223-7747 |
| language | English |
| publishDate | 2025-04-01 |
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| spelling | doaj-art-20e455bedb024a249803758e19e63ccc2025-08-20T03:08:53ZengMDPI AGPlants2223-77472025-04-01147107310.3390/plants14071073Insights into the Genetics Underlying the Resistance to Root-Knot Nematode Reproduction in the Common Bean Ouro NegroAna M. Pesqueira0Ana M. González1Teresa Barragán-Lozano2María S. Arnedo3Rafael Lozano4Marta Santalla5Grupo de Genética del Desarrollo de Plantas, Misión Biológica de Galicia-CSIC, P.O. Box 28, 36080 Pontevedra, SpainGrupo de Genética del Desarrollo de Plantas, Misión Biológica de Galicia-CSIC, P.O. Box 28, 36080 Pontevedra, SpainCentro de Investigación en Biotecnología Agroalimentaria (CIAIMBITAL), Universidad de Almería, 04120 Almería, SpainSemillas Ramiro Arnedo, Las Norias de Daza, 04716 Almería, SpainCentro de Investigación en Biotecnología Agroalimentaria (CIAIMBITAL), Universidad de Almería, 04120 Almería, SpainGrupo de Genética del Desarrollo de Plantas, Misión Biológica de Galicia-CSIC, P.O. Box 28, 36080 Pontevedra, SpainRoot-knot nematodes (RKNs, <i>Meloidogyne</i> spp.) have become the major yield-limiting biological factor in common bean production in many warmer-climate regions such as the south of Europe. Broadening the genetic base of resistance in elite common bean cultivars is the most effective and environmentally friendly method for managing this disease. Toward this goal, F<sub>1</sub>, F<sub>2</sub>, and F<sub>3</sub> populations from crosses between susceptible snap beans (Helda and Perona) and the resistant Ouro Negro cultivar were phenotyped for <i>M. incognita</i> and <i>M. javanica</i>-induced root-galling (GI) and egg mass production (EM) in controlled growth chamber infection assays. F<sub>1</sub> progenies showed a susceptible response to both RKN isolates, with high GI and EM values, indicating a recessive inheritance of nematode resistance. The estimates for broad-sense heritability for GI and EM in the F<sub>2</sub> Helda × Ouro Negro population infected with <i>M. incognita</i> were 0.62 and 0.54, respectively. RKN resistance in Ouro Negro is largely controlled by partial to overdominant genetic effects and that susceptibility factor leads recessive resistance. The minimum number of genes involved in nematode resistance was estimated to be about two or three. In agreement, genetic analysis of F<sub>2</sub> segregating populations supported duplicate recessive epistasis as the inheritance pattern involved in the resistance provided by the Ouro Negro cultivar. Ouro Negro is an important resource for broadening RKN resistance in elite common bean cultivars.https://www.mdpi.com/2223-7747/14/7/1073<i>Phaseolus vulgaris</i>geneticsresistance<i>Meloidogyne</i>root-knot nematode |
| spellingShingle | Ana M. Pesqueira Ana M. González Teresa Barragán-Lozano María S. Arnedo Rafael Lozano Marta Santalla Insights into the Genetics Underlying the Resistance to Root-Knot Nematode Reproduction in the Common Bean Ouro Negro Plants <i>Phaseolus vulgaris</i> genetics resistance <i>Meloidogyne</i> root-knot nematode |
| title | Insights into the Genetics Underlying the Resistance to Root-Knot Nematode Reproduction in the Common Bean Ouro Negro |
| title_full | Insights into the Genetics Underlying the Resistance to Root-Knot Nematode Reproduction in the Common Bean Ouro Negro |
| title_fullStr | Insights into the Genetics Underlying the Resistance to Root-Knot Nematode Reproduction in the Common Bean Ouro Negro |
| title_full_unstemmed | Insights into the Genetics Underlying the Resistance to Root-Knot Nematode Reproduction in the Common Bean Ouro Negro |
| title_short | Insights into the Genetics Underlying the Resistance to Root-Knot Nematode Reproduction in the Common Bean Ouro Negro |
| title_sort | insights into the genetics underlying the resistance to root knot nematode reproduction in the common bean ouro negro |
| topic | <i>Phaseolus vulgaris</i> genetics resistance <i>Meloidogyne</i> root-knot nematode |
| url | https://www.mdpi.com/2223-7747/14/7/1073 |
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