Combined exosome of adipose-derived mesenchymal stem cell and hyaluronic acid delays early osteoarthritis progression of ovine sheep model: Clinical, radiographic, macroscopic and microscopic evaluation [version 3; peer review: 2 approved, 1 not approved]
Background Current treatment of osteoarthritis (OA) mainly focused on treating symptoms. Exosome from Adipose-derived Mesenchymal Stem Cell (Ad-MSC) have been shown to delay degenerative process. This study aimed to investigate the clinical, radiological and histological impact of combined intra-art...
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F1000 Research Ltd
2025-02-01
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| author | Marcel Prasetyo Radiana Dhewayani Antarianto Tri Isyani Tungga Dewi Bambang Pontjo Priosoeryanto Trevino Aristakus Pakasi Angela Jennifer Tantry Alida Harahap Ismail Hadisoebroto Dilogo Jessica Fiolin Ludwig Andribert Powantia Pontoh |
| author_facet | Marcel Prasetyo Radiana Dhewayani Antarianto Tri Isyani Tungga Dewi Bambang Pontjo Priosoeryanto Trevino Aristakus Pakasi Angela Jennifer Tantry Alida Harahap Ismail Hadisoebroto Dilogo Jessica Fiolin Ludwig Andribert Powantia Pontoh |
| author_sort | Marcel Prasetyo |
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| description | Background Current treatment of osteoarthritis (OA) mainly focused on treating symptoms. Exosome from Adipose-derived Mesenchymal Stem Cell (Ad-MSC) have been shown to delay degenerative process. This study aimed to investigate the clinical, radiological and histological impact of combined intra-articular (IA) hyaluronic acid (HA) and exosome Ad-MSCs in-vivo using a larger animal model with low-grade OA. Methods Eighteen male Ovies aries sheep underwent total lateral meniscectomy and conventional radiography was performed to confirm low-grade OA after 6 weeks. The sheep were divided into three groups, Group 1 (G1; n=6) received thrice exosome injections, G2 (n=6) received twice HA injection, and G3 (n=6) received both treatments with a 1-week interval after 10 days of meniscectomy. Clinical evaluations were conducted using the Clinical Lameness Score (CLS), radiographic with X-ray using OA score by Innes et al, while macroscopic evaluation by Osteoarthritis Research Society International (OARSI) scores. Results Lameness parameter scored lowest in G3 significantly (2.0±0.0 VS 2.7±0.52 VS 2.7±0.52; p=0.024) at the second month although the overall CLS score did not significantly differ at the 3rd month. The best improvement of conventional total OA radiographic score at the 3rd month compared to all groups (5.2±1.17 vs 6.3±0.82 vs 6.7±1.03; p=0.053). Macroscopic OARSI evaluation showed no difference (p=0.711). Conclusions Combined repeated exosome Ad-MSC and HA IA injection proven to delay OA progression, however longer duration of follow up is required to evaluate its long-term effect. |
| format | Article |
| id | doaj-art-20c7ac505b8c4b45973f3c17bbe3d030 |
| institution | OA Journals |
| issn | 2046-1402 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | F1000 Research Ltd |
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| spelling | doaj-art-20c7ac505b8c4b45973f3c17bbe3d0302025-08-20T02:15:19ZengF1000 Research LtdF1000Research2046-14022025-02-011310.12688/f1000research.147309.3178040Combined exosome of adipose-derived mesenchymal stem cell and hyaluronic acid delays early osteoarthritis progression of ovine sheep model: Clinical, radiographic, macroscopic and microscopic evaluation [version 3; peer review: 2 approved, 1 not approved]Marcel Prasetyo0https://orcid.org/0000-0002-7796-3871Radiana Dhewayani Antarianto1Tri Isyani Tungga Dewi2Bambang Pontjo Priosoeryanto3Trevino Aristakus Pakasi4Angela Jennifer Tantry5https://orcid.org/0009-0000-3558-7954Alida Harahap6Ismail Hadisoebroto Dilogo7Jessica Fiolin8Ludwig Andribert Powantia Pontoh9https://orcid.org/0000-0001-8856-1977Department of Radiology, Faculty of Medicine, Universitas Indonesia, Central Jakarta, DKI Jakarta, 10430, IndonesiaDepartment of Histology, Faculty of Medicine, Universitas Indonesia, Central Jakarta, DKI Jakarta, 10430, IndonesiaSchool of Veterinary Medicine and Biomedical Science, Institut Pertanian Bogor, Bogor, West Java, 16680, IndonesiaSchool of Veterinary Medicine and Biomedical Science, Institut Pertanian Bogor, Bogor, West Java, 16680, IndonesiaDepartment of Community Medicine, Faculty of Medicine, Universitas Indonesia, Central Jakarta, DKI Jakarta, 10430, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Science, Universitas Indonesia, Depok, West Java, 16424, IndonesiaDoctoral Program of Medical Science, Faculty of Medicine, Universitas Indonesia, Central Jakarta, DKI Jakarta, 10430, IndonesiaOrthopaedic and Traumatology, Faculty of Medicine, Universitas Indonesia, Centra Jakarta, DKI Jakarta, 10430, IndonesiaDoctoral Program of Medical Science, Faculty of Medicine, Universitas Indonesia, Central Jakarta, DKI Jakarta, 10430, IndonesiaOrthopaedic and Traumatology, Faculty of Medicine, Universitas Indonesia, Centra Jakarta, DKI Jakarta, 10430, IndonesiaBackground Current treatment of osteoarthritis (OA) mainly focused on treating symptoms. Exosome from Adipose-derived Mesenchymal Stem Cell (Ad-MSC) have been shown to delay degenerative process. This study aimed to investigate the clinical, radiological and histological impact of combined intra-articular (IA) hyaluronic acid (HA) and exosome Ad-MSCs in-vivo using a larger animal model with low-grade OA. Methods Eighteen male Ovies aries sheep underwent total lateral meniscectomy and conventional radiography was performed to confirm low-grade OA after 6 weeks. The sheep were divided into three groups, Group 1 (G1; n=6) received thrice exosome injections, G2 (n=6) received twice HA injection, and G3 (n=6) received both treatments with a 1-week interval after 10 days of meniscectomy. Clinical evaluations were conducted using the Clinical Lameness Score (CLS), radiographic with X-ray using OA score by Innes et al, while macroscopic evaluation by Osteoarthritis Research Society International (OARSI) scores. Results Lameness parameter scored lowest in G3 significantly (2.0±0.0 VS 2.7±0.52 VS 2.7±0.52; p=0.024) at the second month although the overall CLS score did not significantly differ at the 3rd month. The best improvement of conventional total OA radiographic score at the 3rd month compared to all groups (5.2±1.17 vs 6.3±0.82 vs 6.7±1.03; p=0.053). Macroscopic OARSI evaluation showed no difference (p=0.711). Conclusions Combined repeated exosome Ad-MSC and HA IA injection proven to delay OA progression, however longer duration of follow up is required to evaluate its long-term effect.https://f1000research.com/articles/13-494/v3exosome adipose-derived Mesenchymal Stem Cell (Ad-MSC) early osteoarthritis (OA) clinical lameness score radiographic OA score Osteoarthritis Research Society International (OARSI) scoreeng |
| spellingShingle | Marcel Prasetyo Radiana Dhewayani Antarianto Tri Isyani Tungga Dewi Bambang Pontjo Priosoeryanto Trevino Aristakus Pakasi Angela Jennifer Tantry Alida Harahap Ismail Hadisoebroto Dilogo Jessica Fiolin Ludwig Andribert Powantia Pontoh Combined exosome of adipose-derived mesenchymal stem cell and hyaluronic acid delays early osteoarthritis progression of ovine sheep model: Clinical, radiographic, macroscopic and microscopic evaluation [version 3; peer review: 2 approved, 1 not approved] F1000Research exosome adipose-derived Mesenchymal Stem Cell (Ad-MSC) early osteoarthritis (OA) clinical lameness score radiographic OA score Osteoarthritis Research Society International (OARSI) score eng |
| title | Combined exosome of adipose-derived mesenchymal stem cell and hyaluronic acid delays early osteoarthritis progression of ovine sheep model: Clinical, radiographic, macroscopic and microscopic evaluation [version 3; peer review: 2 approved, 1 not approved] |
| title_full | Combined exosome of adipose-derived mesenchymal stem cell and hyaluronic acid delays early osteoarthritis progression of ovine sheep model: Clinical, radiographic, macroscopic and microscopic evaluation [version 3; peer review: 2 approved, 1 not approved] |
| title_fullStr | Combined exosome of adipose-derived mesenchymal stem cell and hyaluronic acid delays early osteoarthritis progression of ovine sheep model: Clinical, radiographic, macroscopic and microscopic evaluation [version 3; peer review: 2 approved, 1 not approved] |
| title_full_unstemmed | Combined exosome of adipose-derived mesenchymal stem cell and hyaluronic acid delays early osteoarthritis progression of ovine sheep model: Clinical, radiographic, macroscopic and microscopic evaluation [version 3; peer review: 2 approved, 1 not approved] |
| title_short | Combined exosome of adipose-derived mesenchymal stem cell and hyaluronic acid delays early osteoarthritis progression of ovine sheep model: Clinical, radiographic, macroscopic and microscopic evaluation [version 3; peer review: 2 approved, 1 not approved] |
| title_sort | combined exosome of adipose derived mesenchymal stem cell and hyaluronic acid delays early osteoarthritis progression of ovine sheep model clinical radiographic macroscopic and microscopic evaluation version 3 peer review 2 approved 1 not approved |
| topic | exosome adipose-derived Mesenchymal Stem Cell (Ad-MSC) early osteoarthritis (OA) clinical lameness score radiographic OA score Osteoarthritis Research Society International (OARSI) score eng |
| url | https://f1000research.com/articles/13-494/v3 |
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