Linking Complement C3 and B Cells in Nasal Polyposis

Nasal polyposis often is characterized by a persistent inflammation of the sinonasal mucosa, disease recurrence after medical or surgical intervention, and asthma comorbidity. Dysregulated complement activation may contribute to immunologic alterations and disease. To date, there is only scattered k...

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Main Authors: Ulrike Werner, Axel Künstner, Maren Drenckhan, Ralph Pries, Karl-Ludwig Bruchhage, Hauke S. Busch, Claus Bachert, Barbara Wollenberg
Format: Article
Language:English
Published: Wiley 2020-01-01
Series:Journal of Immunology Research
Online Access:http://dx.doi.org/10.1155/2020/4832189
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author Ulrike Werner
Axel Künstner
Maren Drenckhan
Ralph Pries
Karl-Ludwig Bruchhage
Hauke S. Busch
Claus Bachert
Barbara Wollenberg
author_facet Ulrike Werner
Axel Künstner
Maren Drenckhan
Ralph Pries
Karl-Ludwig Bruchhage
Hauke S. Busch
Claus Bachert
Barbara Wollenberg
author_sort Ulrike Werner
collection DOAJ
description Nasal polyposis often is characterized by a persistent inflammation of the sinonasal mucosa, disease recurrence after medical or surgical intervention, and asthma comorbidity. Dysregulated complement activation may contribute to immunologic alterations and disease. To date, there is only scattered knowledge on the source and regulation of the central complement factors in the pathogenesis of nasal polyps. Here, we aim to study complement signatures, especially the C3-C3aR axis, and focus on cellular sources and targets in nasal polyps. Expression of complement factors, including C3, C5, and the anaphylatoxin receptors, was analyzed in nasal polyp tissue samples, the corresponding inferior turbinates, and healthy controls using transcriptomic methods and protein measurements. Distinct patterns of complement expression were found in nasal polyps compared to controls, characterized by an increased C3 activation and an increase in C3aR-bearing cells. In contrast, no difference was shown for epithelial-dependent C3 production. Besides low intracellular C3-expression levels for lymphocytes in general, we could identify an enlarged B lymphocyte population in nasal polyps displaying high amounts of intracellular C3. Our data suggest a prominent role for the C3-C3aR-axis in nasal polyps and, for the first time, describe a B cell population containing high levels of intracellular C3, suggesting a new role of B cells in the maintenance of the inflammation by complement.
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issn 2314-8861
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spelling doaj-art-20c4543ddb7f4ce684b223ab817748252025-02-03T06:46:35ZengWileyJournal of Immunology Research2314-88612314-71562020-01-01202010.1155/2020/48321894832189Linking Complement C3 and B Cells in Nasal PolyposisUlrike Werner0Axel Künstner1Maren Drenckhan2Ralph Pries3Karl-Ludwig Bruchhage4Hauke S. Busch5Claus Bachert6Barbara Wollenberg7Department of Otorhinolaryngology, University Hospital Schleswig-Holstein, Campus Lübeck, GermanyGroup of Medical Systems Biology, Lübeck Institute of Experimental Dermatology, University of Lübeck, GermanyDepartment of Otorhinolaryngology, University Hospital Schleswig-Holstein, Campus Lübeck, GermanyDepartment of Otorhinolaryngology, University Hospital Schleswig-Holstein, Campus Lübeck, GermanyDepartment of Otorhinolaryngology, University Hospital Schleswig-Holstein, Campus Lübeck, GermanyGroup of Medical Systems Biology, Lübeck Institute of Experimental Dermatology, University of Lübeck, GermanyUpper Airway Research Laboratory, Department of Otorhinolaryngology, Ghent University Hospital, BelgiumDepartment of Otorhinolaryngology, University Hospital Schleswig-Holstein, Campus Lübeck, GermanyNasal polyposis often is characterized by a persistent inflammation of the sinonasal mucosa, disease recurrence after medical or surgical intervention, and asthma comorbidity. Dysregulated complement activation may contribute to immunologic alterations and disease. To date, there is only scattered knowledge on the source and regulation of the central complement factors in the pathogenesis of nasal polyps. Here, we aim to study complement signatures, especially the C3-C3aR axis, and focus on cellular sources and targets in nasal polyps. Expression of complement factors, including C3, C5, and the anaphylatoxin receptors, was analyzed in nasal polyp tissue samples, the corresponding inferior turbinates, and healthy controls using transcriptomic methods and protein measurements. Distinct patterns of complement expression were found in nasal polyps compared to controls, characterized by an increased C3 activation and an increase in C3aR-bearing cells. In contrast, no difference was shown for epithelial-dependent C3 production. Besides low intracellular C3-expression levels for lymphocytes in general, we could identify an enlarged B lymphocyte population in nasal polyps displaying high amounts of intracellular C3. Our data suggest a prominent role for the C3-C3aR-axis in nasal polyps and, for the first time, describe a B cell population containing high levels of intracellular C3, suggesting a new role of B cells in the maintenance of the inflammation by complement.http://dx.doi.org/10.1155/2020/4832189
spellingShingle Ulrike Werner
Axel Künstner
Maren Drenckhan
Ralph Pries
Karl-Ludwig Bruchhage
Hauke S. Busch
Claus Bachert
Barbara Wollenberg
Linking Complement C3 and B Cells in Nasal Polyposis
Journal of Immunology Research
title Linking Complement C3 and B Cells in Nasal Polyposis
title_full Linking Complement C3 and B Cells in Nasal Polyposis
title_fullStr Linking Complement C3 and B Cells in Nasal Polyposis
title_full_unstemmed Linking Complement C3 and B Cells in Nasal Polyposis
title_short Linking Complement C3 and B Cells in Nasal Polyposis
title_sort linking complement c3 and b cells in nasal polyposis
url http://dx.doi.org/10.1155/2020/4832189
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