Expression of Matrix Metalloproteinases and Their Tissue Inhibitors in Peripheral Blood Leukocytes and Plasma of Children with Nonalcoholic Fatty Liver Disease
Gene expression profiles of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) were evaluated in peripheral blood leukocytes of children with nonalcoholic fatty liver disease (NAFLD). Gene expression patterns were correlated with their plasma protein counterparts, systemic paramete...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2020/8327945 |
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| author | Joanna B. Trojanek Jacek Michałkiewicz Renata Grzywa-Czuba Wojciech Jańczyk Lidia Gackowska Izabela Kubiszewska Anna Helmin-Basa Aldona Wierzbicka-Rucińska Mieczysław Szalecki Piotr Socha |
| author_facet | Joanna B. Trojanek Jacek Michałkiewicz Renata Grzywa-Czuba Wojciech Jańczyk Lidia Gackowska Izabela Kubiszewska Anna Helmin-Basa Aldona Wierzbicka-Rucińska Mieczysław Szalecki Piotr Socha |
| author_sort | Joanna B. Trojanek |
| collection | DOAJ |
| description | Gene expression profiles of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) were evaluated in peripheral blood leukocytes of children with nonalcoholic fatty liver disease (NAFLD). Gene expression patterns were correlated with their plasma protein counterparts, systemic parameters of liver injury, and selected markers of inflammation. The MMP-2, MMP-9, MMP-12, MMP-14, TIMP-1, TIMP-2, TGF-β, and IL-6 transcripts levels were tested by the real-time PCR. Plasma concentrations of MMP-9, TIMP-1, MMP-9/TIMP-1 ratio, MMP-2/TIMP-2 ratio, sCD14, leptin, resistin, IL-1 beta, and IL-6 and serum markers of liver injury were estimated by ELISA. The MMP-9, TIMP-2 expression levels, plasma amounts of MMP-9, TIMP-1, and the MMP-9/TIMP-1 ratio were increased in children with NAFLD. Concentrations of AST, ALT, GGT, and leptin were elevated in serum patients with NAFLD, while concentration of other inflammatory or liver injury markers was unchanged. The MMP-2 and MMP-9 levels correlated with serum liver injury parameters (ALT and GGT concentrations, respectively); there were no other correlations between MMP/TIMP gene expression profiles, their plasma counterparts, and serum inflammatory markers. Association of MMP-2 and MMP-9 expression with serum liver injury parameters (ALT, GGT) may suggest leukocyte engagement in the early stages of NAFLD development which possibly precedes subsequent systemic inflammatory responses. |
| format | Article |
| id | doaj-art-20bfd01562d3454c8a3c596457f20653 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-20bfd01562d3454c8a3c596457f206532025-02-03T01:05:07ZengWileyMediators of Inflammation0962-93511466-18612020-01-01202010.1155/2020/83279458327945Expression of Matrix Metalloproteinases and Their Tissue Inhibitors in Peripheral Blood Leukocytes and Plasma of Children with Nonalcoholic Fatty Liver DiseaseJoanna B. Trojanek0Jacek Michałkiewicz1Renata Grzywa-Czuba2Wojciech Jańczyk3Lidia Gackowska4Izabela Kubiszewska5Anna Helmin-Basa6Aldona Wierzbicka-Rucińska7Mieczysław Szalecki8Piotr Socha9Department of Microbiology and Clinical Immunology, The Children’s Memorial Health Institute, Warsaw, PolandDepartment of Microbiology and Clinical Immunology, The Children’s Memorial Health Institute, Warsaw, PolandDepartment of Microbiology and Clinical Immunology, The Children’s Memorial Health Institute, Warsaw, PolandDepartment of Gastroenterology, Hepatology, Nutritional Disorders and Paediatric, The Children’s Memorial Health Institute, Warsaw, PolandCollegium Medicum Nicolaus Copernicus University, Bydgoszcz, PolandCollegium Medicum Nicolaus Copernicus University, Bydgoszcz, PolandCollegium Medicum Nicolaus Copernicus University, Bydgoszcz, PolandDepartment of Biochemistry, Radioimmunology and Experimental Medicine, The Children’s Memorial Health Institute, Warsaw, PolandDepartment of Endocrinology and Diabetology, The Children’s Memorial Health Institute, Warsaw, PolandDepartment of Gastroenterology, Hepatology, Nutritional Disorders and Paediatric, The Children’s Memorial Health Institute, Warsaw, PolandGene expression profiles of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) were evaluated in peripheral blood leukocytes of children with nonalcoholic fatty liver disease (NAFLD). Gene expression patterns were correlated with their plasma protein counterparts, systemic parameters of liver injury, and selected markers of inflammation. The MMP-2, MMP-9, MMP-12, MMP-14, TIMP-1, TIMP-2, TGF-β, and IL-6 transcripts levels were tested by the real-time PCR. Plasma concentrations of MMP-9, TIMP-1, MMP-9/TIMP-1 ratio, MMP-2/TIMP-2 ratio, sCD14, leptin, resistin, IL-1 beta, and IL-6 and serum markers of liver injury were estimated by ELISA. The MMP-9, TIMP-2 expression levels, plasma amounts of MMP-9, TIMP-1, and the MMP-9/TIMP-1 ratio were increased in children with NAFLD. Concentrations of AST, ALT, GGT, and leptin were elevated in serum patients with NAFLD, while concentration of other inflammatory or liver injury markers was unchanged. The MMP-2 and MMP-9 levels correlated with serum liver injury parameters (ALT and GGT concentrations, respectively); there were no other correlations between MMP/TIMP gene expression profiles, their plasma counterparts, and serum inflammatory markers. Association of MMP-2 and MMP-9 expression with serum liver injury parameters (ALT, GGT) may suggest leukocyte engagement in the early stages of NAFLD development which possibly precedes subsequent systemic inflammatory responses.http://dx.doi.org/10.1155/2020/8327945 |
| spellingShingle | Joanna B. Trojanek Jacek Michałkiewicz Renata Grzywa-Czuba Wojciech Jańczyk Lidia Gackowska Izabela Kubiszewska Anna Helmin-Basa Aldona Wierzbicka-Rucińska Mieczysław Szalecki Piotr Socha Expression of Matrix Metalloproteinases and Their Tissue Inhibitors in Peripheral Blood Leukocytes and Plasma of Children with Nonalcoholic Fatty Liver Disease Mediators of Inflammation |
| title | Expression of Matrix Metalloproteinases and Their Tissue Inhibitors in Peripheral Blood Leukocytes and Plasma of Children with Nonalcoholic Fatty Liver Disease |
| title_full | Expression of Matrix Metalloproteinases and Their Tissue Inhibitors in Peripheral Blood Leukocytes and Plasma of Children with Nonalcoholic Fatty Liver Disease |
| title_fullStr | Expression of Matrix Metalloproteinases and Their Tissue Inhibitors in Peripheral Blood Leukocytes and Plasma of Children with Nonalcoholic Fatty Liver Disease |
| title_full_unstemmed | Expression of Matrix Metalloproteinases and Their Tissue Inhibitors in Peripheral Blood Leukocytes and Plasma of Children with Nonalcoholic Fatty Liver Disease |
| title_short | Expression of Matrix Metalloproteinases and Their Tissue Inhibitors in Peripheral Blood Leukocytes and Plasma of Children with Nonalcoholic Fatty Liver Disease |
| title_sort | expression of matrix metalloproteinases and their tissue inhibitors in peripheral blood leukocytes and plasma of children with nonalcoholic fatty liver disease |
| url | http://dx.doi.org/10.1155/2020/8327945 |
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