Chronocoulometric Quantum Dot Aptasensor for SARS‐CoV‐2 Nucleocapsid Protein Biomarker
Abstract For the first time, a novel mercaptosuccinic acid‐capped tungsten telluride quantum dot (MSA‐WTe3‐QD)‐based electrochemical aptasensor was designed for the determination of SARS‐CoV‐2 N‐protein in synthetic human serum, plasma and urine. The MSA‐WTe3‐QD which exhibited spherical morphology...
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2024-07-01
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| Series: | ChemElectroChem |
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| Online Access: | https://doi.org/10.1002/celc.202400118 |
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| author | Nelia A. Sanga Marlon Oranzie Zandile Leve Kaylin C. Januarie Kefilwe V. Mokwebo Onyinyechi V. Uhuo Natasha Ross Samantha F. Douman Keagan Pokpas Emmanuel I. Iwuoha |
| author_facet | Nelia A. Sanga Marlon Oranzie Zandile Leve Kaylin C. Januarie Kefilwe V. Mokwebo Onyinyechi V. Uhuo Natasha Ross Samantha F. Douman Keagan Pokpas Emmanuel I. Iwuoha |
| author_sort | Nelia A. Sanga |
| collection | DOAJ |
| description | Abstract For the first time, a novel mercaptosuccinic acid‐capped tungsten telluride quantum dot (MSA‐WTe3‐QD)‐based electrochemical aptasensor was designed for the determination of SARS‐CoV‐2 N‐protein in synthetic human serum, plasma and urine. The MSA‐WTe3‐QD which exhibited spherical morphology and an average diameter of 3.56 nm were synthesized through a bottom‐up approach. The aptasensor was prepared by firstly immobilizing MSA‐WTe3‐QD on a screen‐printed carbon electrode (SPCE) by using the 1‐ethyl‐3‐(3‐dimethylaminopropyl)carbodiimide/N‐hydroxysuccinimide (EDC/NHS) coupling chemistry. Thereafter, amine‐terminated 90‐mer ssDNA aptamer was deposited on the modified SPCE and left to cure to form the aptasensor. Using the chronocoulometry signal transduction technique, the aptasensor's calibration with N‐protein gave a linear range value of 1–9 pg.mL−1 and a limit of detection (LOD) value of 0.08 pg.mL−1. The LOD value of the aptasensor was 8 times lower than the LOD value of 0.71 pg.mL−1 obtained with a commercial N‐protein ELISA kit. When tested in simulated real samples the aptasensor recovery rates of 95–103.33 % were obtained. In the presence of various interferents, the aptasensor displayed good selectivity to N‐protein. |
| format | Article |
| id | doaj-art-20bda43c4c144db19d3929f1157518d4 |
| institution | OA Journals |
| issn | 2196-0216 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | ChemElectroChem |
| spelling | doaj-art-20bda43c4c144db19d3929f1157518d42025-08-20T01:55:31ZengWiley-VCHChemElectroChem2196-02162024-07-011114n/an/a10.1002/celc.202400118Chronocoulometric Quantum Dot Aptasensor for SARS‐CoV‐2 Nucleocapsid Protein BiomarkerNelia A. Sanga0Marlon Oranzie1Zandile Leve2Kaylin C. Januarie3Kefilwe V. Mokwebo4Onyinyechi V. Uhuo5Natasha Ross6Samantha F. Douman7Keagan Pokpas8Emmanuel I. Iwuoha9SensorLab (University of the Western Cape Sensor Laboratories) University of the Western Cape Robert Sobukwe Road, Bellville 7535 Cape Town South AfricaSensorLab (University of the Western Cape Sensor Laboratories) University of the Western Cape Robert Sobukwe Road, Bellville 7535 Cape Town South AfricaSensorLab (University of the Western Cape Sensor Laboratories) University of the Western Cape Robert Sobukwe Road, Bellville 7535 Cape Town South AfricaSensorLab (University of the Western Cape Sensor Laboratories) University of the Western Cape Robert Sobukwe Road, Bellville 7535 Cape Town South AfricaSensorLab (University of the Western Cape Sensor Laboratories) University of the Western Cape Robert Sobukwe Road, Bellville 7535 Cape Town South AfricaSensorLab (University of the Western Cape Sensor Laboratories) University of the Western Cape Robert Sobukwe Road, Bellville 7535 Cape Town South AfricaSensorLab (University of the Western Cape Sensor Laboratories) University of the Western Cape Robert Sobukwe Road, Bellville 7535 Cape Town South AfricaSensorLab (University of the Western Cape Sensor Laboratories) University of the Western Cape Robert Sobukwe Road, Bellville 7535 Cape Town South AfricaSensorLab (University of the Western Cape Sensor Laboratories) University of the Western Cape Robert Sobukwe Road, Bellville 7535 Cape Town South AfricaSensorLab (University of the Western Cape Sensor Laboratories) University of the Western Cape Robert Sobukwe Road, Bellville 7535 Cape Town South AfricaAbstract For the first time, a novel mercaptosuccinic acid‐capped tungsten telluride quantum dot (MSA‐WTe3‐QD)‐based electrochemical aptasensor was designed for the determination of SARS‐CoV‐2 N‐protein in synthetic human serum, plasma and urine. The MSA‐WTe3‐QD which exhibited spherical morphology and an average diameter of 3.56 nm were synthesized through a bottom‐up approach. The aptasensor was prepared by firstly immobilizing MSA‐WTe3‐QD on a screen‐printed carbon electrode (SPCE) by using the 1‐ethyl‐3‐(3‐dimethylaminopropyl)carbodiimide/N‐hydroxysuccinimide (EDC/NHS) coupling chemistry. Thereafter, amine‐terminated 90‐mer ssDNA aptamer was deposited on the modified SPCE and left to cure to form the aptasensor. Using the chronocoulometry signal transduction technique, the aptasensor's calibration with N‐protein gave a linear range value of 1–9 pg.mL−1 and a limit of detection (LOD) value of 0.08 pg.mL−1. The LOD value of the aptasensor was 8 times lower than the LOD value of 0.71 pg.mL−1 obtained with a commercial N‐protein ELISA kit. When tested in simulated real samples the aptasensor recovery rates of 95–103.33 % were obtained. In the presence of various interferents, the aptasensor displayed good selectivity to N‐protein.https://doi.org/10.1002/celc.202400118Chronocoulometric aptasensorlabel-free detectionnucleocapsid protein (N-protein)quantum dotSARS-CoV-2 |
| spellingShingle | Nelia A. Sanga Marlon Oranzie Zandile Leve Kaylin C. Januarie Kefilwe V. Mokwebo Onyinyechi V. Uhuo Natasha Ross Samantha F. Douman Keagan Pokpas Emmanuel I. Iwuoha Chronocoulometric Quantum Dot Aptasensor for SARS‐CoV‐2 Nucleocapsid Protein Biomarker ChemElectroChem Chronocoulometric aptasensor label-free detection nucleocapsid protein (N-protein) quantum dot SARS-CoV-2 |
| title | Chronocoulometric Quantum Dot Aptasensor for SARS‐CoV‐2 Nucleocapsid Protein Biomarker |
| title_full | Chronocoulometric Quantum Dot Aptasensor for SARS‐CoV‐2 Nucleocapsid Protein Biomarker |
| title_fullStr | Chronocoulometric Quantum Dot Aptasensor for SARS‐CoV‐2 Nucleocapsid Protein Biomarker |
| title_full_unstemmed | Chronocoulometric Quantum Dot Aptasensor for SARS‐CoV‐2 Nucleocapsid Protein Biomarker |
| title_short | Chronocoulometric Quantum Dot Aptasensor for SARS‐CoV‐2 Nucleocapsid Protein Biomarker |
| title_sort | chronocoulometric quantum dot aptasensor for sars cov 2 nucleocapsid protein biomarker |
| topic | Chronocoulometric aptasensor label-free detection nucleocapsid protein (N-protein) quantum dot SARS-CoV-2 |
| url | https://doi.org/10.1002/celc.202400118 |
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