Assessing Adventitious Matches of Non-Donors Related to True Contributors

Relatives share more DNA with one another than unrelated individuals. Therefore, there is an increased risk of close relatives (e.g., siblings or parents and children of true donors) adventitiously ‘matching’ DNA mixtures to which they are not a true contributor. One such method of addressing relati...

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Main Authors: Kaitlin Huffman, Lilliana Moreno
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Biomolecules
Subjects:
Online Access:https://www.mdpi.com/2218-273X/15/3/398
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author Kaitlin Huffman
Lilliana Moreno
author_facet Kaitlin Huffman
Lilliana Moreno
author_sort Kaitlin Huffman
collection DOAJ
description Relatives share more DNA with one another than unrelated individuals. Therefore, there is an increased risk of close relatives (e.g., siblings or parents and children of true donors) adventitiously ‘matching’ DNA mixtures to which they are not a true contributor. One such method of addressing relatives is to utilize alternative propositions within probabilistic genotyping software (e.g., STRmix<sup>TM</sup>). As the number of related individuals within a mixture increases, so does the potential for adventitious matches of related non-donors to the mixture due to increased allele sharing. The extent to which siblings and parents/children of true donors result in adventitious matches to mixtures in which they are non-donors is presented as well as the impact of overestimating the number of contributors (NOC) when related donors are in question.
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series Biomolecules
spelling doaj-art-20a3e4b577bf4f059225bb0727d6e9482025-08-20T03:43:02ZengMDPI AGBiomolecules2218-273X2025-03-0115339810.3390/biom15030398Assessing Adventitious Matches of Non-Donors Related to True ContributorsKaitlin Huffman0Lilliana Moreno1DNA Support Unit, Federal Bureau of Investigation Laboratory, Quantico, VA 22135, USADNA Support Unit, Federal Bureau of Investigation Laboratory, Quantico, VA 22135, USARelatives share more DNA with one another than unrelated individuals. Therefore, there is an increased risk of close relatives (e.g., siblings or parents and children of true donors) adventitiously ‘matching’ DNA mixtures to which they are not a true contributor. One such method of addressing relatives is to utilize alternative propositions within probabilistic genotyping software (e.g., STRmix<sup>TM</sup>). As the number of related individuals within a mixture increases, so does the potential for adventitious matches of related non-donors to the mixture due to increased allele sharing. The extent to which siblings and parents/children of true donors result in adventitious matches to mixtures in which they are non-donors is presented as well as the impact of overestimating the number of contributors (NOC) when related donors are in question.https://www.mdpi.com/2218-273X/15/3/398probabilistic genotypingcomplex mixturesrelated non-donors
spellingShingle Kaitlin Huffman
Lilliana Moreno
Assessing Adventitious Matches of Non-Donors Related to True Contributors
Biomolecules
probabilistic genotyping
complex mixtures
related non-donors
title Assessing Adventitious Matches of Non-Donors Related to True Contributors
title_full Assessing Adventitious Matches of Non-Donors Related to True Contributors
title_fullStr Assessing Adventitious Matches of Non-Donors Related to True Contributors
title_full_unstemmed Assessing Adventitious Matches of Non-Donors Related to True Contributors
title_short Assessing Adventitious Matches of Non-Donors Related to True Contributors
title_sort assessing adventitious matches of non donors related to true contributors
topic probabilistic genotyping
complex mixtures
related non-donors
url https://www.mdpi.com/2218-273X/15/3/398
work_keys_str_mv AT kaitlinhuffman assessingadventitiousmatchesofnondonorsrelatedtotruecontributors
AT lillianamoreno assessingadventitiousmatchesofnondonorsrelatedtotruecontributors