Aerobic exercise and cannabidiol supplementation modulate prostate Akt gene expression in high-fat diet-fed rats: Mechanistic insights into obesity-driven prostate cancer risk
Epidemiological evidence indicates that obesity is associated with accelerated prostate cancer progression and elevated mortality rates. However, the underlying physiological mechanisms linking obesity to prostate cancer pathogenesis remain unclear. This study aimed to examine the effects of six wee...
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2025-03-01
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| Series: | Journal of Exercise & Organ Cross Talk |
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| Online Access: | https://www.jeoct.com/article_222046_b52364b2c7dec6d0a88729f57f429092.pdf |
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| author | Marzieh Hafezi Eirdmousa Mandana Gholami Heshmatollah Parsian Hossein Abed-Natanzi |
| author_facet | Marzieh Hafezi Eirdmousa Mandana Gholami Heshmatollah Parsian Hossein Abed-Natanzi |
| author_sort | Marzieh Hafezi Eirdmousa |
| collection | DOAJ |
| description | Epidemiological evidence indicates that obesity is associated with accelerated prostate cancer progression and elevated mortality rates. However, the underlying physiological mechanisms linking obesity to prostate cancer pathogenesis remain unclear. This study aimed to examine the effects of six weeks of moderate-intensity aerobic training combined with cannabidiol (CBD) supplementation on prostate protein kinase B (Akt) gene expression in high-fat diet (HFD)-induced obese male rats. Forty male Wistar rats (240 ± 20 g) were randomly assigned to five experimental groups: healthy control, HFD, HFD+exercise, HFD+CBD, HFD+exercise and CBD (n=8 per group). CBD was administered via oral gavage at 100 mg/kg, five days per week for six weeks. The aerobic exercise protocol consisted of treadmill running at progressive intensities, ranging from 15 m/min for 30 min to 27 m/min for 60 min, five days per week for six weeks. Following the intervention, prostate tissue was extracted for quantitative analysis of Akt gene expression. Statistical analysis revealed that aerobic exercise significantly modulated Akt gene expression (P=0.015). Similarly, CBD administration exerted a significant effect on Akt expression (P=0.004). However, no significant interactive effect was observed between aerobic exercise and CBD (P=0.068). These findings suggest that both aerobic exercise and CBD independently influence Akt signaling pathways, potentially through anti-inflammatory mechanisms, which may contribute to prostate cancer prevention. Further research is warranted to elucidate the precise molecular interactions involved. |
| format | Article |
| id | doaj-art-209bf51f7c9b4826966eda4b2be149fd |
| institution | Kabale University |
| issn | 2783-2074 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | JEOCT publisher |
| record_format | Article |
| series | Journal of Exercise & Organ Cross Talk |
| spelling | doaj-art-209bf51f7c9b4826966eda4b2be149fd2025-08-20T03:34:57ZengJEOCT publisherJournal of Exercise & Organ Cross Talk2783-20742025-03-015171210.22122/jeoct.2025.525710.1154222046Aerobic exercise and cannabidiol supplementation modulate prostate Akt gene expression in high-fat diet-fed rats: Mechanistic insights into obesity-driven prostate cancer riskMarzieh Hafezi Eirdmousa0Mandana Gholami1Heshmatollah Parsian2Hossein Abed-Natanzi3Department of Physical Education and Sports Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.Department of Physical Education and Sports Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.Department of Physical Education and Sport Sciences, Shahr-E- Qods Branch, Islamic Azad University, Tehran, Iran.Department of Physical Education and Sports Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.Epidemiological evidence indicates that obesity is associated with accelerated prostate cancer progression and elevated mortality rates. However, the underlying physiological mechanisms linking obesity to prostate cancer pathogenesis remain unclear. This study aimed to examine the effects of six weeks of moderate-intensity aerobic training combined with cannabidiol (CBD) supplementation on prostate protein kinase B (Akt) gene expression in high-fat diet (HFD)-induced obese male rats. Forty male Wistar rats (240 ± 20 g) were randomly assigned to five experimental groups: healthy control, HFD, HFD+exercise, HFD+CBD, HFD+exercise and CBD (n=8 per group). CBD was administered via oral gavage at 100 mg/kg, five days per week for six weeks. The aerobic exercise protocol consisted of treadmill running at progressive intensities, ranging from 15 m/min for 30 min to 27 m/min for 60 min, five days per week for six weeks. Following the intervention, prostate tissue was extracted for quantitative analysis of Akt gene expression. Statistical analysis revealed that aerobic exercise significantly modulated Akt gene expression (P=0.015). Similarly, CBD administration exerted a significant effect on Akt expression (P=0.004). However, no significant interactive effect was observed between aerobic exercise and CBD (P=0.068). These findings suggest that both aerobic exercise and CBD independently influence Akt signaling pathways, potentially through anti-inflammatory mechanisms, which may contribute to prostate cancer prevention. Further research is warranted to elucidate the precise molecular interactions involved.https://www.jeoct.com/article_222046_b52364b2c7dec6d0a88729f57f429092.pdfmoderate-intensity aerobic exercisecannabisprostatehigh-fat dietakt gene |
| spellingShingle | Marzieh Hafezi Eirdmousa Mandana Gholami Heshmatollah Parsian Hossein Abed-Natanzi Aerobic exercise and cannabidiol supplementation modulate prostate Akt gene expression in high-fat diet-fed rats: Mechanistic insights into obesity-driven prostate cancer risk Journal of Exercise & Organ Cross Talk moderate-intensity aerobic exercise cannabis prostate high-fat diet akt gene |
| title | Aerobic exercise and cannabidiol supplementation modulate prostate Akt gene expression in high-fat diet-fed rats: Mechanistic insights into obesity-driven prostate cancer risk |
| title_full | Aerobic exercise and cannabidiol supplementation modulate prostate Akt gene expression in high-fat diet-fed rats: Mechanistic insights into obesity-driven prostate cancer risk |
| title_fullStr | Aerobic exercise and cannabidiol supplementation modulate prostate Akt gene expression in high-fat diet-fed rats: Mechanistic insights into obesity-driven prostate cancer risk |
| title_full_unstemmed | Aerobic exercise and cannabidiol supplementation modulate prostate Akt gene expression in high-fat diet-fed rats: Mechanistic insights into obesity-driven prostate cancer risk |
| title_short | Aerobic exercise and cannabidiol supplementation modulate prostate Akt gene expression in high-fat diet-fed rats: Mechanistic insights into obesity-driven prostate cancer risk |
| title_sort | aerobic exercise and cannabidiol supplementation modulate prostate akt gene expression in high fat diet fed rats mechanistic insights into obesity driven prostate cancer risk |
| topic | moderate-intensity aerobic exercise cannabis prostate high-fat diet akt gene |
| url | https://www.jeoct.com/article_222046_b52364b2c7dec6d0a88729f57f429092.pdf |
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