Flexibility in PAM recognition expands DNA targeting in xCas9

Flexibility in PAM recognition expands DNA targeting in xCas9

xCas9 is an evolved variant of the CRISPR-Cas9 genome editing system, engineered to improve specificity and reduce undesired off-target effects. How xCas9 expands the DNA targeting capability of Cas9 by recognising a series of alternative protospacer adjacent motif (PAM) sequences while ignoring oth...

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Bibliographic Details
Main Authors: Kazi A Hossain, Lukasz Nierzwicki, Modesto Orozco, Jacek Czub, Giulia Palermo
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2025-02-01
Series:eLife
Subjects:
CRISPR-Cas9
RNA
genome editing
entropy
protein-DNA
protein dynamics
Online Access:https://elifesciences.org/articles/102538
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