Male and female behavioral variability and morphine response in C57BL/6J, DBA/2J, and their BXD progeny following chronic stress exposure

Abstract Drug addiction is a multifactorial syndrome in which genetic predispositions and exposure to environmental stressors constitute major risk factors for the early onset, escalation, and relapse of addictive behaviors. While it is well known that stress plays a key role in drug addiction, the...

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Main Authors: Carole Morel, Lyonna F. Parise, Yentl Y. Van der Zee, Orna Issler, Min Cai, Caleb J. Browne, Anthony Blando, Katherine B. LeClair, Antonio V. Aubry, Sherod Haynes, Robert W. Williams, Megan K. Mulligan, Scott J. Russo, Eric J. Nestler, Ming-Hu Han
Format: Article
Language:English
Published: Nature Portfolio 2024-12-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-024-80767-7
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author Carole Morel
Lyonna F. Parise
Yentl Y. Van der Zee
Orna Issler
Min Cai
Caleb J. Browne
Anthony Blando
Katherine B. LeClair
Antonio V. Aubry
Sherod Haynes
Robert W. Williams
Megan K. Mulligan
Scott J. Russo
Eric J. Nestler
Ming-Hu Han
author_facet Carole Morel
Lyonna F. Parise
Yentl Y. Van der Zee
Orna Issler
Min Cai
Caleb J. Browne
Anthony Blando
Katherine B. LeClair
Antonio V. Aubry
Sherod Haynes
Robert W. Williams
Megan K. Mulligan
Scott J. Russo
Eric J. Nestler
Ming-Hu Han
author_sort Carole Morel
collection DOAJ
description Abstract Drug addiction is a multifactorial syndrome in which genetic predispositions and exposure to environmental stressors constitute major risk factors for the early onset, escalation, and relapse of addictive behaviors. While it is well known that stress plays a key role in drug addiction, the genetic factors that make certain individuals particularly sensitive to stress and, thereby, more vulnerable to becoming addicted are unknown. In an effort to test a complex set of gene x environment interactions—specifically gene x chronic stress—here we leveraged a systems genetics resource: BXD recombinant inbred mice (BXD5, BXD8, BXD14, BXD22, BXD29, and BXD32) and their parental mouse lines, C57BL/6J and DBA/2J. Utilizing the chronic social defeat stress (CSDS) and chronic variable stress (CVS) paradigms, we first showed sexual dimorphism in social and exploratory behaviors between the mouse strains. Further, we observed an interaction between genetic background and vulnerability to prolonged exposure to non-social stressors. Finally, we found that DBA/2J and C57BL/6J mice pre-exposed to stress displayed differences in morphine sensitivity. Our results support the hypothesis that genetic variation influences chronic stress-induced behavioral outcomes such as social and approach-avoidance behaviors, reward responses, as well as morphine sensitivity, and is likely to modulate the development of drug addiction.
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spelling doaj-art-207efff159214bad9dc045ff45b7f2492025-08-20T02:43:25ZengNature PortfolioScientific Reports2045-23222024-12-0114111210.1038/s41598-024-80767-7Male and female behavioral variability and morphine response in C57BL/6J, DBA/2J, and their BXD progeny following chronic stress exposureCarole Morel0Lyonna F. Parise1Yentl Y. Van der Zee2Orna Issler3Min Cai4Caleb J. Browne5Anthony Blando6Katherine B. LeClair7Antonio V. Aubry8Sherod Haynes9Robert W. Williams10Megan K. Mulligan11Scott J. Russo12Eric J. Nestler13Ming-Hu Han14Department of Pharmacological Sciences, Icahn School of Medicine at Mount SinaiFriedman Brain Institute, and Center for Affective Neuroscience, Icahn School of Medicine at Mount SinaiFriedman Brain Institute, and Center for Affective Neuroscience, Icahn School of Medicine at Mount SinaiFriedman Brain Institute, and Center for Affective Neuroscience, Icahn School of Medicine at Mount SinaiDepartment of Pharmacological Sciences, Icahn School of Medicine at Mount SinaiFriedman Brain Institute, and Center for Affective Neuroscience, Icahn School of Medicine at Mount SinaiDepartment of Pharmacological Sciences, Icahn School of Medicine at Mount SinaiFriedman Brain Institute, and Center for Affective Neuroscience, Icahn School of Medicine at Mount SinaiFriedman Brain Institute, and Center for Affective Neuroscience, Icahn School of Medicine at Mount SinaiDepartment of Pharmacological Sciences, Icahn School of Medicine at Mount SinaiDepartment of Genetics, Genomics and Informatics, University of Tennessee Health Science CenterDepartment of Genetics, Genomics and Informatics, University of Tennessee Health Science CenterFriedman Brain Institute, and Center for Affective Neuroscience, Icahn School of Medicine at Mount SinaiFriedman Brain Institute, and Center for Affective Neuroscience, Icahn School of Medicine at Mount SinaiDepartment of Pharmacological Sciences, Icahn School of Medicine at Mount SinaiAbstract Drug addiction is a multifactorial syndrome in which genetic predispositions and exposure to environmental stressors constitute major risk factors for the early onset, escalation, and relapse of addictive behaviors. While it is well known that stress plays a key role in drug addiction, the genetic factors that make certain individuals particularly sensitive to stress and, thereby, more vulnerable to becoming addicted are unknown. In an effort to test a complex set of gene x environment interactions—specifically gene x chronic stress—here we leveraged a systems genetics resource: BXD recombinant inbred mice (BXD5, BXD8, BXD14, BXD22, BXD29, and BXD32) and their parental mouse lines, C57BL/6J and DBA/2J. Utilizing the chronic social defeat stress (CSDS) and chronic variable stress (CVS) paradigms, we first showed sexual dimorphism in social and exploratory behaviors between the mouse strains. Further, we observed an interaction between genetic background and vulnerability to prolonged exposure to non-social stressors. Finally, we found that DBA/2J and C57BL/6J mice pre-exposed to stress displayed differences in morphine sensitivity. Our results support the hypothesis that genetic variation influences chronic stress-induced behavioral outcomes such as social and approach-avoidance behaviors, reward responses, as well as morphine sensitivity, and is likely to modulate the development of drug addiction.https://doi.org/10.1038/s41598-024-80767-7
spellingShingle Carole Morel
Lyonna F. Parise
Yentl Y. Van der Zee
Orna Issler
Min Cai
Caleb J. Browne
Anthony Blando
Katherine B. LeClair
Antonio V. Aubry
Sherod Haynes
Robert W. Williams
Megan K. Mulligan
Scott J. Russo
Eric J. Nestler
Ming-Hu Han
Male and female behavioral variability and morphine response in C57BL/6J, DBA/2J, and their BXD progeny following chronic stress exposure
Scientific Reports
title Male and female behavioral variability and morphine response in C57BL/6J, DBA/2J, and their BXD progeny following chronic stress exposure
title_full Male and female behavioral variability and morphine response in C57BL/6J, DBA/2J, and their BXD progeny following chronic stress exposure
title_fullStr Male and female behavioral variability and morphine response in C57BL/6J, DBA/2J, and their BXD progeny following chronic stress exposure
title_full_unstemmed Male and female behavioral variability and morphine response in C57BL/6J, DBA/2J, and their BXD progeny following chronic stress exposure
title_short Male and female behavioral variability and morphine response in C57BL/6J, DBA/2J, and their BXD progeny following chronic stress exposure
title_sort male and female behavioral variability and morphine response in c57bl 6j dba 2j and their bxd progeny following chronic stress exposure
url https://doi.org/10.1038/s41598-024-80767-7
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