Construction of <i>Bacillus</i>–<i>Pseudomonas</i> Synthetic Communities and Development of Bio-Nursery Substrates
With the rapid development of ecological agriculture and organic products, there is an urgent need to reduce the use of fertilizers and pesticides by producing bio-nursery substrates containing multifunctional microbial communities. In this study, beneficial <i>Pseudomonas</i> strains we...
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2024-09-01
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| author | Qisheng Li Qing Li Xiaodong Yin Yanyan Jia Kai Yang Jiamin Song Yang Che Ke Li Zhangrong Wen Dalu Gu Xiaofeng Du Wenfei Yang |
| author_facet | Qisheng Li Qing Li Xiaodong Yin Yanyan Jia Kai Yang Jiamin Song Yang Che Ke Li Zhangrong Wen Dalu Gu Xiaofeng Du Wenfei Yang |
| author_sort | Qisheng Li |
| collection | DOAJ |
| description | With the rapid development of ecological agriculture and organic products, there is an urgent need to reduce the use of fertilizers and pesticides by producing bio-nursery substrates containing multifunctional microbial communities. In this study, beneficial <i>Pseudomonas</i> strains were screened from the rhizosphere of muskmelon (<i>Cucumis melo</i> L.) pre-inoculated with <i>Bacillus velezensis</i> R1-3. The ability of the strain to dissolve phosphorus and produce indole-3-acetic acid (IAA), the effect of the strain on seed germination rate, and the antagonism with R1-3 were determined. Four strains of beneficial <i>Pseudomonas</i> strains that had no antagonistic effect against R1-3 were obtained and formed a <i>Bacillus</i>–<i>Pseudomonas</i> community. The seedling effect of biological substrates containing the <i>Bacillus</i>–<i>Pseudomonas</i> community was evaluated using a seedling pot experiment and a pot experiment. The results showed that the phosphorus solubilization range of all <i>Pseudomonas</i> strains was 86.32–459.48 mg L<sup>−1</sup> and the IAA production range was 2.98–11.86 mg L<sup>−1</sup>. There was a significant negative correlation between the amount of phosphorus dissolved in the fermentation solution and pH. Combined with the results of the seed germination rate and antagonism test, the strains R1-3 + HY-S7, R1-3 + HY-S25, R1-3 + HY-S36, and R1-3 + HY-S70 were selected for the seedling pot experiment and the pot experiment. The results of the two-season seedling culture and two-season pot experiments showed that the bio-nursery substrates containing the bacterial community R1-3 + HY-S70 significantly promoted the growth of muskmelon seedlings, improved plant height, maximum leaf length, and fresh weight, and were significantly better than single bacterial and control treatments at increasing plant height and fresh weight. Finally, the bacterial community R1-3 + HY-S70 was established as the optimal combination for developing biological seedling substrates. Based on 16S rDNA gene sequence analysis, the strain HY-S70 was preliminarily identified as <i>Pseudomonas moraviensis</i>. |
| format | Article |
| id | doaj-art-207c3b74d53b49c3a5052caae815ec5a |
| institution | OA Journals |
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| language | English |
| publishDate | 2024-09-01 |
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| series | Agronomy |
| spelling | doaj-art-207c3b74d53b49c3a5052caae815ec5a2025-08-20T01:56:09ZengMDPI AGAgronomy2073-43952024-09-01149217910.3390/agronomy14092179Construction of <i>Bacillus</i>–<i>Pseudomonas</i> Synthetic Communities and Development of Bio-Nursery SubstratesQisheng Li0Qing Li1Xiaodong Yin2Yanyan Jia3Kai Yang4Jiamin Song5Yang Che6Ke Li7Zhangrong Wen8Dalu Gu9Xiaofeng Du10Wenfei Yang11Huaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu, Jiangsu Academy of Agricultural Sciences, Huaian 223001, ChinaHuaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu, Jiangsu Academy of Agricultural Sciences, Huaian 223001, ChinaHuaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu, Jiangsu Academy of Agricultural Sciences, Huaian 223001, ChinaHuaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu, Jiangsu Academy of Agricultural Sciences, Huaian 223001, ChinaHuai’an District Agricultural Technology Extension Center, Huaian 223232, ChinaHuaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu, Jiangsu Academy of Agricultural Sciences, Huaian 223001, ChinaHuaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu, Jiangsu Academy of Agricultural Sciences, Huaian 223001, ChinaHuaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu, Jiangsu Academy of Agricultural Sciences, Huaian 223001, ChinaHuaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu, Jiangsu Academy of Agricultural Sciences, Huaian 223001, ChinaHuaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu, Jiangsu Academy of Agricultural Sciences, Huaian 223001, ChinaHuaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu, Jiangsu Academy of Agricultural Sciences, Huaian 223001, ChinaHuaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu, Jiangsu Academy of Agricultural Sciences, Huaian 223001, ChinaWith the rapid development of ecological agriculture and organic products, there is an urgent need to reduce the use of fertilizers and pesticides by producing bio-nursery substrates containing multifunctional microbial communities. In this study, beneficial <i>Pseudomonas</i> strains were screened from the rhizosphere of muskmelon (<i>Cucumis melo</i> L.) pre-inoculated with <i>Bacillus velezensis</i> R1-3. The ability of the strain to dissolve phosphorus and produce indole-3-acetic acid (IAA), the effect of the strain on seed germination rate, and the antagonism with R1-3 were determined. Four strains of beneficial <i>Pseudomonas</i> strains that had no antagonistic effect against R1-3 were obtained and formed a <i>Bacillus</i>–<i>Pseudomonas</i> community. The seedling effect of biological substrates containing the <i>Bacillus</i>–<i>Pseudomonas</i> community was evaluated using a seedling pot experiment and a pot experiment. The results showed that the phosphorus solubilization range of all <i>Pseudomonas</i> strains was 86.32–459.48 mg L<sup>−1</sup> and the IAA production range was 2.98–11.86 mg L<sup>−1</sup>. There was a significant negative correlation between the amount of phosphorus dissolved in the fermentation solution and pH. Combined with the results of the seed germination rate and antagonism test, the strains R1-3 + HY-S7, R1-3 + HY-S25, R1-3 + HY-S36, and R1-3 + HY-S70 were selected for the seedling pot experiment and the pot experiment. The results of the two-season seedling culture and two-season pot experiments showed that the bio-nursery substrates containing the bacterial community R1-3 + HY-S70 significantly promoted the growth of muskmelon seedlings, improved plant height, maximum leaf length, and fresh weight, and were significantly better than single bacterial and control treatments at increasing plant height and fresh weight. Finally, the bacterial community R1-3 + HY-S70 was established as the optimal combination for developing biological seedling substrates. Based on 16S rDNA gene sequence analysis, the strain HY-S70 was preliminarily identified as <i>Pseudomonas moraviensis</i>.https://www.mdpi.com/2073-4395/14/9/2179<i>Bacillus</i> sp.<i>Pseudomonas</i> sp.synthetic microbial communitybiological seedling substratesphosphate solubilizing ability |
| spellingShingle | Qisheng Li Qing Li Xiaodong Yin Yanyan Jia Kai Yang Jiamin Song Yang Che Ke Li Zhangrong Wen Dalu Gu Xiaofeng Du Wenfei Yang Construction of <i>Bacillus</i>–<i>Pseudomonas</i> Synthetic Communities and Development of Bio-Nursery Substrates Agronomy <i>Bacillus</i> sp. <i>Pseudomonas</i> sp. synthetic microbial community biological seedling substrates phosphate solubilizing ability |
| title | Construction of <i>Bacillus</i>–<i>Pseudomonas</i> Synthetic Communities and Development of Bio-Nursery Substrates |
| title_full | Construction of <i>Bacillus</i>–<i>Pseudomonas</i> Synthetic Communities and Development of Bio-Nursery Substrates |
| title_fullStr | Construction of <i>Bacillus</i>–<i>Pseudomonas</i> Synthetic Communities and Development of Bio-Nursery Substrates |
| title_full_unstemmed | Construction of <i>Bacillus</i>–<i>Pseudomonas</i> Synthetic Communities and Development of Bio-Nursery Substrates |
| title_short | Construction of <i>Bacillus</i>–<i>Pseudomonas</i> Synthetic Communities and Development of Bio-Nursery Substrates |
| title_sort | construction of i bacillus i i pseudomonas i synthetic communities and development of bio nursery substrates |
| topic | <i>Bacillus</i> sp. <i>Pseudomonas</i> sp. synthetic microbial community biological seedling substrates phosphate solubilizing ability |
| url | https://www.mdpi.com/2073-4395/14/9/2179 |
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