Parvovirus B19 NS1 protein induces synovitis mimicking rheumatoid arthritis
Abstract This study aims to investigate the role of parvovirus B19 infection in the pathogenesis of rheumatoid arthritis (RA). B19-NS1 DNA was cloned into the LentiORF pLEX-MCS vector, then co-transfected with the pLEX-MCS-NS1 vector and packaging system to produce LEX-NS1 lentiviral stock. LEX was...
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Nature Portfolio
2025-08-01
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| Online Access: | https://doi.org/10.1038/s41598-025-11900-3 |
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| author | Chang-Yi Yen Chia-Chun Tseng Chia‐Hui Lin Yuan‐Zhao Lin Ruei‐Nian Li Pin-Yi Wang Kuan-Yu Chen Cheng-Chin Wu Tsan-Teng Ou Chung-Jen Chen Yu-Peng Liu Jeng-Hsien Yen |
| author_facet | Chang-Yi Yen Chia-Chun Tseng Chia‐Hui Lin Yuan‐Zhao Lin Ruei‐Nian Li Pin-Yi Wang Kuan-Yu Chen Cheng-Chin Wu Tsan-Teng Ou Chung-Jen Chen Yu-Peng Liu Jeng-Hsien Yen |
| author_sort | Chang-Yi Yen |
| collection | DOAJ |
| description | Abstract This study aims to investigate the role of parvovirus B19 infection in the pathogenesis of rheumatoid arthritis (RA). B19-NS1 DNA was cloned into the LentiORF pLEX-MCS vector, then co-transfected with the pLEX-MCS-NS1 vector and packaging system to produce LEX-NS1 lentiviral stock. LEX was also prepared as the control. Transduction of LEX-NS1 or LEX lentivirus into the SW982 cell line then proceeded, and total RNA was extracted for RNA-seq. Pathway analysis and comparison of pathway status between this study and GEO datasets were performed, demonstrating that acute phase response signaling, NRF2-mediated oxidative stress response, and mTOR signaling pathways were activated after the transduction of LEX-NS1 into the SW982 cell line, although the interferon signaling pathway was inhibited. The comparison of all pathways using hierarchical clustering showed that the pathway status after transduction of B19-NS1 was closer to RA than that in osteoarthritis. Similar findings can also be found in cellular immune, humoral immune, cytokine, apoptosis, cellular growth, cellular stress, growth factor, and intracellular pathways. In conclusion, parvovirus B19-NS1 can induce inflammation of the synovium mimicking synovitis in RA; therefore, parvovirus B19 infection might be one of the trigger factors in the pathogenesis of RA. |
| format | Article |
| id | doaj-art-2079ee8908bc421fb1866c0dbfd2c2ba |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-2079ee8908bc421fb1866c0dbfd2c2ba2025-08-20T04:03:02ZengNature PortfolioScientific Reports2045-23222025-08-0115111110.1038/s41598-025-11900-3Parvovirus B19 NS1 protein induces synovitis mimicking rheumatoid arthritisChang-Yi Yen0Chia-Chun Tseng1Chia‐Hui Lin2Yuan‐Zhao Lin3Ruei‐Nian Li4Pin-Yi Wang5Kuan-Yu Chen6Cheng-Chin Wu7Tsan-Teng Ou8Chung-Jen Chen9Yu-Peng Liu10Jeng-Hsien Yen11Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University HospitalDivision of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University HospitalDivision of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University HospitalDivision of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University HospitalDepartment of Biomedical Science and Environmental Biology, College of Life Science, Kaohsiung Medical UniversityDepartment of Internal Medicine, Kaohsiung Municipal Siaogang HospitalGraduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical UniversityDivision of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University HospitalDivision of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University HospitalDivision of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University HospitalGraduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical UniversityDivision of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University HospitalAbstract This study aims to investigate the role of parvovirus B19 infection in the pathogenesis of rheumatoid arthritis (RA). B19-NS1 DNA was cloned into the LentiORF pLEX-MCS vector, then co-transfected with the pLEX-MCS-NS1 vector and packaging system to produce LEX-NS1 lentiviral stock. LEX was also prepared as the control. Transduction of LEX-NS1 or LEX lentivirus into the SW982 cell line then proceeded, and total RNA was extracted for RNA-seq. Pathway analysis and comparison of pathway status between this study and GEO datasets were performed, demonstrating that acute phase response signaling, NRF2-mediated oxidative stress response, and mTOR signaling pathways were activated after the transduction of LEX-NS1 into the SW982 cell line, although the interferon signaling pathway was inhibited. The comparison of all pathways using hierarchical clustering showed that the pathway status after transduction of B19-NS1 was closer to RA than that in osteoarthritis. Similar findings can also be found in cellular immune, humoral immune, cytokine, apoptosis, cellular growth, cellular stress, growth factor, and intracellular pathways. In conclusion, parvovirus B19-NS1 can induce inflammation of the synovium mimicking synovitis in RA; therefore, parvovirus B19 infection might be one of the trigger factors in the pathogenesis of RA.https://doi.org/10.1038/s41598-025-11900-3Parvovirus B19Rheumatoid arthritisSynovitisRNA-seq |
| spellingShingle | Chang-Yi Yen Chia-Chun Tseng Chia‐Hui Lin Yuan‐Zhao Lin Ruei‐Nian Li Pin-Yi Wang Kuan-Yu Chen Cheng-Chin Wu Tsan-Teng Ou Chung-Jen Chen Yu-Peng Liu Jeng-Hsien Yen Parvovirus B19 NS1 protein induces synovitis mimicking rheumatoid arthritis Scientific Reports Parvovirus B19 Rheumatoid arthritis Synovitis RNA-seq |
| title | Parvovirus B19 NS1 protein induces synovitis mimicking rheumatoid arthritis |
| title_full | Parvovirus B19 NS1 protein induces synovitis mimicking rheumatoid arthritis |
| title_fullStr | Parvovirus B19 NS1 protein induces synovitis mimicking rheumatoid arthritis |
| title_full_unstemmed | Parvovirus B19 NS1 protein induces synovitis mimicking rheumatoid arthritis |
| title_short | Parvovirus B19 NS1 protein induces synovitis mimicking rheumatoid arthritis |
| title_sort | parvovirus b19 ns1 protein induces synovitis mimicking rheumatoid arthritis |
| topic | Parvovirus B19 Rheumatoid arthritis Synovitis RNA-seq |
| url | https://doi.org/10.1038/s41598-025-11900-3 |
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