Comprehensive comparative metabolome study of a large collection of Corsican bryophytes
IntroductionBryophytes are non-vascular plants that appeared on Earth before vascular plants. More than 24,000 species are reported worldwide, and only a small proportion have been studied. However, part of their biosynthetic potential has been unveiled and more than 1,600 terpenoids have been detec...
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| Format: | Article |
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1470307/full |
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| author | Anaïs Pannequin Anaïs Pannequin Alain Muselli Laurence Marcourt Laurence Marcourt Emerson Ferreira Queiroz Emerson Ferreira Queiroz Luis-Manuel Quiros-Guerrero Luis-Manuel Quiros-Guerrero Yoshinori Asakawa Miwa Dounoue-Kubo Jean-Luc Wolfender Jean-Luc Wolfender |
| author_facet | Anaïs Pannequin Anaïs Pannequin Alain Muselli Laurence Marcourt Laurence Marcourt Emerson Ferreira Queiroz Emerson Ferreira Queiroz Luis-Manuel Quiros-Guerrero Luis-Manuel Quiros-Guerrero Yoshinori Asakawa Miwa Dounoue-Kubo Jean-Luc Wolfender Jean-Luc Wolfender |
| author_sort | Anaïs Pannequin |
| collection | DOAJ |
| description | IntroductionBryophytes are non-vascular plants that appeared on Earth before vascular plants. More than 24,000 species are reported worldwide, and only a small proportion have been studied. However, part of their biosynthetic potential has been unveiled and more than 1,600 terpenoids have been detected and identified. The study of bryophytes faces challenges due to their small size, and sociology, making it difficult to collect large amounts of uncontaminated samples. Additionally, their chemical specificity and the scarcity of chemical data specific to this branch further complicate their study. Traditionally, research on bryophytes has focused only on specific species or classes of compounds. MethodsIn contrast, our work proposes the first untargeted metabolite profiling investigation of a large collection of bryophytes (63 species) mainly issued from Corsican biodiversity. Metabolite profiling was performed by UHPLC-HRMS/MS and the data was extensively annotated using computational tools and molecular networking. This allowed us to describe in detail the chemical space covered by our collection and to establish comparisons between all the moss and liverwort species available. To validate some of the structural annotations, 3 liverworts (Frullania tamarisci, Pellia epiphylla, Plagiochila porelloides) and 2 mosses (Antitrichia curtipendula and Dicranum scoparium), available in larger quantities were fractionated using high-resolution semi-preparative HPLC, yielding 20 pure compounds. Five of them were newly discovered.Results and discussionThis study highlights the main compositional differences between mosses and liverworts at the chemical class level. By analyzing given molecular network clusters, specific biosynthetic features or compounds that are characteristic of certain species are highlighted and discussed in detail. |
| format | Article |
| id | doaj-art-20765cf1179d4557b505b366792b014a |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-20765cf1179d4557b505b366792b014a2025-01-07T04:12:58ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-01-011510.3389/fpls.2024.14703071470307Comprehensive comparative metabolome study of a large collection of Corsican bryophytesAnaïs Pannequin0Anaïs Pannequin1Alain Muselli2Laurence Marcourt3Laurence Marcourt4Emerson Ferreira Queiroz5Emerson Ferreira Queiroz6Luis-Manuel Quiros-Guerrero7Luis-Manuel Quiros-Guerrero8Yoshinori Asakawa9Miwa Dounoue-Kubo10Jean-Luc Wolfender11Jean-Luc Wolfender12Université de Corse, Unité Mixte de Recherche du Centre national de la recherche scientifique (UMR CNRS) SPE 6134, Laboratoire Chimie des Produits Naturels, Corte, FranceInstitute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, Geneva, SwitzerlandUniversité de Corse, Unité Mixte de Recherche du Centre national de la recherche scientifique (UMR CNRS) SPE 6134, Laboratoire Chimie des Produits Naturels, Corte, FranceInstitute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, Geneva, SwitzerlandSchool of Pharmaceutical Sciences, University of Geneva, Geneva, SwitzerlandInstitute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, Geneva, SwitzerlandSchool of Pharmaceutical Sciences, University of Geneva, Geneva, SwitzerlandInstitute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, Geneva, SwitzerlandSchool of Pharmaceutical Sciences, University of Geneva, Geneva, SwitzerlandFaculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima, JapanFaculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima, JapanInstitute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, Geneva, SwitzerlandSchool of Pharmaceutical Sciences, University of Geneva, Geneva, SwitzerlandIntroductionBryophytes are non-vascular plants that appeared on Earth before vascular plants. More than 24,000 species are reported worldwide, and only a small proportion have been studied. However, part of their biosynthetic potential has been unveiled and more than 1,600 terpenoids have been detected and identified. The study of bryophytes faces challenges due to their small size, and sociology, making it difficult to collect large amounts of uncontaminated samples. Additionally, their chemical specificity and the scarcity of chemical data specific to this branch further complicate their study. Traditionally, research on bryophytes has focused only on specific species or classes of compounds. MethodsIn contrast, our work proposes the first untargeted metabolite profiling investigation of a large collection of bryophytes (63 species) mainly issued from Corsican biodiversity. Metabolite profiling was performed by UHPLC-HRMS/MS and the data was extensively annotated using computational tools and molecular networking. This allowed us to describe in detail the chemical space covered by our collection and to establish comparisons between all the moss and liverwort species available. To validate some of the structural annotations, 3 liverworts (Frullania tamarisci, Pellia epiphylla, Plagiochila porelloides) and 2 mosses (Antitrichia curtipendula and Dicranum scoparium), available in larger quantities were fractionated using high-resolution semi-preparative HPLC, yielding 20 pure compounds. Five of them were newly discovered.Results and discussionThis study highlights the main compositional differences between mosses and liverworts at the chemical class level. By analyzing given molecular network clusters, specific biosynthetic features or compounds that are characteristic of certain species are highlighted and discussed in detail.https://www.frontiersin.org/articles/10.3389/fpls.2024.1470307/fullbryophytesliverwortsmossesmetabolomicsnatural productsmolecular networking |
| spellingShingle | Anaïs Pannequin Anaïs Pannequin Alain Muselli Laurence Marcourt Laurence Marcourt Emerson Ferreira Queiroz Emerson Ferreira Queiroz Luis-Manuel Quiros-Guerrero Luis-Manuel Quiros-Guerrero Yoshinori Asakawa Miwa Dounoue-Kubo Jean-Luc Wolfender Jean-Luc Wolfender Comprehensive comparative metabolome study of a large collection of Corsican bryophytes Frontiers in Plant Science bryophytes liverworts mosses metabolomics natural products molecular networking |
| title | Comprehensive comparative metabolome study of a large collection of Corsican bryophytes |
| title_full | Comprehensive comparative metabolome study of a large collection of Corsican bryophytes |
| title_fullStr | Comprehensive comparative metabolome study of a large collection of Corsican bryophytes |
| title_full_unstemmed | Comprehensive comparative metabolome study of a large collection of Corsican bryophytes |
| title_short | Comprehensive comparative metabolome study of a large collection of Corsican bryophytes |
| title_sort | comprehensive comparative metabolome study of a large collection of corsican bryophytes |
| topic | bryophytes liverworts mosses metabolomics natural products molecular networking |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1470307/full |
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