Automatic target-seeking nanoparticle inhibiting orthotopic drug-resistant colon cancer and liver metastases via regulating cancer cell adhesion and proliferation
Abstract Galectin-3 (Gal-3) plays an important role in adhesion and proliferation of cancer cells. The level of Gal-3 in blood and the expression of Gal-3 in colon cancer tissue are significantly increased in patient with colon cancer. The elevated Gal-3 promotes the migration and drug resistance of...
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| Language: | English |
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BMC
2025-06-01
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| Series: | Journal of Nanobiotechnology |
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| Online Access: | https://doi.org/10.1186/s12951-025-03422-x |
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| author | Shaobo Bai Yang Sun Miao Liu Ying Cheng Qifeng Ji Bangle Zhang Zhifu Yang Siyuan Zhou Daozhou Liu |
| author_facet | Shaobo Bai Yang Sun Miao Liu Ying Cheng Qifeng Ji Bangle Zhang Zhifu Yang Siyuan Zhou Daozhou Liu |
| author_sort | Shaobo Bai |
| collection | DOAJ |
| description | Abstract Galectin-3 (Gal-3) plays an important role in adhesion and proliferation of cancer cells. The level of Gal-3 in blood and the expression of Gal-3 in colon cancer tissue are significantly increased in patient with colon cancer. The elevated Gal-3 promotes the migration and drug resistance of colon cancer. Therefore, Gal-3 is a promising target to inhibit the growth and metastases of cancer cells. Besides, integrin αvβ3, a receptor of Gal-3, is highly expressed in colon cancer cell and blood vessel in colon cancer tissue. In this paper, an automatic target-seeking nanoparticle (SP@MCaP) contained siGal-3 and paris saponin VII (PSVII) was prepared. In vivo, by automatically capturing Gal-3 in the blood circulation, SP@MCaP actively recognized cancer tissue vessel and drug-resistant colon cancer cells with elevated integrin αvβ3 expression, resulting in specifical accumulation in orthotopic drug-resistant colon cancer tissue. SP@MCaP diminished Gal-3 level in serum and orthotopic drug-resistant colon cancer tissue, and then suppressed the proliferation of drug-resistant colon cancer cells. Importantly, SP@MCaP reconstructed the adhesion of drug-resistant colon cancer cells and reversed the immunosuppressive microenvironment in orthotopic drug-resistant colon cancer tissue and liver tissue. Finally, under the synergistic effect of siGal-3 and PSVII, SP@MCaP successfully inhibited the growth of orthotopic drug-resistant colon cancer and its liver metastases. In a word, this paper explored a novel concept of the active co-delivery of siGal-3 and PSVII by modification of nanoparticle, which holds promise for targeted therapy in orthotopic drug-resistant colon cancer and its liver metastases. Graphical abstract First, SP@MCaP automatically captured Gal-3 in the blood, actively recognized cancer tissue vessel and drug-resistant colon cancer cells with elevated integrin αvβ3 expression and specifically accumulated in orthotopic drug-resistant colon cancer tissue. Then, SP@MCaP successfully inhibited the growth of orthotopic drug-resistant colon cancer and its liver metastases by diminishing Gal-3 level in serum and orthotopic drug-resistant colon cancer tissue, suppressing the proliferation of drug-resistant colon cancer cells, reconstructing the adhesion of drug-resistant colon cancer cells and ameliorating the immunosuppressive microenvironment in orthotopic drug-resistant colon cancer tissue and liver tissue |
| format | Article |
| id | doaj-art-207555dd6a4a4e1d8068c6c72203b9c2 |
| institution | DOAJ |
| issn | 1477-3155 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj-art-207555dd6a4a4e1d8068c6c72203b9c22025-08-20T03:10:40ZengBMCJournal of Nanobiotechnology1477-31552025-06-0123112010.1186/s12951-025-03422-xAutomatic target-seeking nanoparticle inhibiting orthotopic drug-resistant colon cancer and liver metastases via regulating cancer cell adhesion and proliferationShaobo Bai0Yang Sun1Miao Liu2Ying Cheng3Qifeng Ji4Bangle Zhang5Zhifu Yang6Siyuan Zhou7Daozhou Liu8Department of Pharmaceutics, School of Pharmacy, Air Force Medical UniversityKey Laboratory of Gastrointestinal Pharmacology of Chinese Materia Medica of the State Administration of Traditional Chinese Medicine, Department of Pharmacology, School of Pharmacy, Air Force Medical UniversityDepartment of Pharmaceutics, School of Pharmacy, Air Force Medical UniversityDepartment of Pharmaceutics, School of Pharmacy, Air Force Medical UniversityDepartment of Pharmaceutics, School of Pharmacy, Air Force Medical UniversityDepartment of Pharmaceutics, School of Pharmacy, Air Force Medical UniversityDepartment of Pharmacy, Xijing Hospital, Air Force Medical UniversityDepartment of Pharmaceutics, School of Pharmacy, Air Force Medical UniversityDepartment of Pharmaceutics, School of Pharmacy, Air Force Medical UniversityAbstract Galectin-3 (Gal-3) plays an important role in adhesion and proliferation of cancer cells. The level of Gal-3 in blood and the expression of Gal-3 in colon cancer tissue are significantly increased in patient with colon cancer. The elevated Gal-3 promotes the migration and drug resistance of colon cancer. Therefore, Gal-3 is a promising target to inhibit the growth and metastases of cancer cells. Besides, integrin αvβ3, a receptor of Gal-3, is highly expressed in colon cancer cell and blood vessel in colon cancer tissue. In this paper, an automatic target-seeking nanoparticle (SP@MCaP) contained siGal-3 and paris saponin VII (PSVII) was prepared. In vivo, by automatically capturing Gal-3 in the blood circulation, SP@MCaP actively recognized cancer tissue vessel and drug-resistant colon cancer cells with elevated integrin αvβ3 expression, resulting in specifical accumulation in orthotopic drug-resistant colon cancer tissue. SP@MCaP diminished Gal-3 level in serum and orthotopic drug-resistant colon cancer tissue, and then suppressed the proliferation of drug-resistant colon cancer cells. Importantly, SP@MCaP reconstructed the adhesion of drug-resistant colon cancer cells and reversed the immunosuppressive microenvironment in orthotopic drug-resistant colon cancer tissue and liver tissue. Finally, under the synergistic effect of siGal-3 and PSVII, SP@MCaP successfully inhibited the growth of orthotopic drug-resistant colon cancer and its liver metastases. In a word, this paper explored a novel concept of the active co-delivery of siGal-3 and PSVII by modification of nanoparticle, which holds promise for targeted therapy in orthotopic drug-resistant colon cancer and its liver metastases. Graphical abstract First, SP@MCaP automatically captured Gal-3 in the blood, actively recognized cancer tissue vessel and drug-resistant colon cancer cells with elevated integrin αvβ3 expression and specifically accumulated in orthotopic drug-resistant colon cancer tissue. Then, SP@MCaP successfully inhibited the growth of orthotopic drug-resistant colon cancer and its liver metastases by diminishing Gal-3 level in serum and orthotopic drug-resistant colon cancer tissue, suppressing the proliferation of drug-resistant colon cancer cells, reconstructing the adhesion of drug-resistant colon cancer cells and ameliorating the immunosuppressive microenvironment in orthotopic drug-resistant colon cancer tissue and liver tissuehttps://doi.org/10.1186/s12951-025-03422-xDrug-resistant colon cancerGalectin-3Cell adhesionParis saponin VIIIntegrin αvβ3 |
| spellingShingle | Shaobo Bai Yang Sun Miao Liu Ying Cheng Qifeng Ji Bangle Zhang Zhifu Yang Siyuan Zhou Daozhou Liu Automatic target-seeking nanoparticle inhibiting orthotopic drug-resistant colon cancer and liver metastases via regulating cancer cell adhesion and proliferation Journal of Nanobiotechnology Drug-resistant colon cancer Galectin-3 Cell adhesion Paris saponin VII Integrin αvβ3 |
| title | Automatic target-seeking nanoparticle inhibiting orthotopic drug-resistant colon cancer and liver metastases via regulating cancer cell adhesion and proliferation |
| title_full | Automatic target-seeking nanoparticle inhibiting orthotopic drug-resistant colon cancer and liver metastases via regulating cancer cell adhesion and proliferation |
| title_fullStr | Automatic target-seeking nanoparticle inhibiting orthotopic drug-resistant colon cancer and liver metastases via regulating cancer cell adhesion and proliferation |
| title_full_unstemmed | Automatic target-seeking nanoparticle inhibiting orthotopic drug-resistant colon cancer and liver metastases via regulating cancer cell adhesion and proliferation |
| title_short | Automatic target-seeking nanoparticle inhibiting orthotopic drug-resistant colon cancer and liver metastases via regulating cancer cell adhesion and proliferation |
| title_sort | automatic target seeking nanoparticle inhibiting orthotopic drug resistant colon cancer and liver metastases via regulating cancer cell adhesion and proliferation |
| topic | Drug-resistant colon cancer Galectin-3 Cell adhesion Paris saponin VII Integrin αvβ3 |
| url | https://doi.org/10.1186/s12951-025-03422-x |
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