Molecular mechanism of nano-vitamin A-mediated regulation of intramuscular fat deposition involving noncoding RNAs in pigs
Abstract Background Intramuscular fat content is a key factor influencing pork quality. Previous studies by our group found that intramuscular injection of Nano VA into newborn piglets significantly increased Intramuscular fat content and improved pork quality. However, the regulatory mechanisms of...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-08-01
|
| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12864-025-11898-y |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849238125570686976 |
|---|---|
| author | Meng Li Wenrun Cai Ruotong Li Wenye Zhao Jiaxin Zhang Xiaoyin Rong Ting Zhang Yang Yang Bugao Li Xiaohong Guo |
| author_facet | Meng Li Wenrun Cai Ruotong Li Wenye Zhao Jiaxin Zhang Xiaoyin Rong Ting Zhang Yang Yang Bugao Li Xiaohong Guo |
| author_sort | Meng Li |
| collection | DOAJ |
| description | Abstract Background Intramuscular fat content is a key factor influencing pork quality. Previous studies by our group found that intramuscular injection of Nano VA into newborn piglets significantly increased Intramuscular fat content and improved pork quality. However, the regulatory mechanisms of noncoding RNAs in Intramuscular fat deposition remain unclear. Results To elucidate the mechanisms by which Nano VA affects pork quality via noncoding RNAs, whole transcriptome sequencing was performed on the longissimus dorsi muscle from control and Nano VA treated groups. A total of 90 differentially expressed circRNAs (DECs), 805 differentially expressed lncRNAs (DELs), and 58 differentially expressed miRNAs (DEmiRs) were screened between the two groups. Among these, 53 DECs were upregulated and 38 were downregulated; 430 DELs were upregulated and 375 were downregulated; 33 DEmiRs were upregulated and 25 were downregulated. GO and KEGG enrichment analyses indicated that the DECs, DELs, and DEmiRs were significantly enriched in pathways including insulin signaling, mTOR signaling, cGMP-PKG signaling, FoxO signaling, retinol metabolism, MAPK signaling, and autophagy. Based on the competitive endogenous RNA regulatory mechanism, a circRNA/lncRNA-miRNA-mRNA network associated with Intramuscular fat deposition was constructed, comprising 9 mRNAs, 3 miRNAs, 50 lncRNAs, and 9 circRNAs, including circRNA2640/circRNA11556-ssc-miR-1296-5p-PIK3AP1, MSTRG.543.4/MSTRG.1281.3 -ssc-miR-17-3p-XIAP, MSTRG.7503.15/circRNA11514-ssc-miR-17-3p-ALDH1A1 and MSTRG.12101.2/ciRNA113-ssc-miR-885-3-GPAT4. These findings suggest that Nano VA likely regulates Intramuscular fat deposition through the above ceRNA network. Conclusions This study reveals that Nano VA promotes porcine Intramuscular fat deposition by modulating an ncRNA mediated ceRNA network, providing a theoretical basis for understanding the molecular mechanisms by which Nano VA improves pork quality. |
| format | Article |
| id | doaj-art-206fe5b058ca40c0a3a7ce4213bfccaa |
| institution | Kabale University |
| issn | 1471-2164 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj-art-206fe5b058ca40c0a3a7ce4213bfccaa2025-08-20T04:01:43ZengBMCBMC Genomics1471-21642025-08-0126111610.1186/s12864-025-11898-yMolecular mechanism of nano-vitamin A-mediated regulation of intramuscular fat deposition involving noncoding RNAs in pigsMeng Li0Wenrun Cai1Ruotong Li2Wenye Zhao3Jiaxin Zhang4Xiaoyin Rong5Ting Zhang6Yang Yang7Bugao Li8Xiaohong Guo9College of Animal Science, Shanxi Agricultural UniversityCollege of Animal Science, Shanxi Agricultural UniversityCollege of Animal Science, Shanxi Agricultural UniversityCollege of Animal Science, Shanxi Agricultural UniversityCollege of Animal Science, Shanxi Agricultural UniversityCollege of Animal Science, Shanxi Agricultural UniversityCollege of Animal Science, Shanxi Agricultural UniversityCollege of Animal Science, Shanxi Agricultural UniversityCollege of Animal Science, Shanxi Agricultural UniversityCollege of Animal Science, Shanxi Agricultural UniversityAbstract Background Intramuscular fat content is a key factor influencing pork quality. Previous studies by our group found that intramuscular injection of Nano VA into newborn piglets significantly increased Intramuscular fat content and improved pork quality. However, the regulatory mechanisms of noncoding RNAs in Intramuscular fat deposition remain unclear. Results To elucidate the mechanisms by which Nano VA affects pork quality via noncoding RNAs, whole transcriptome sequencing was performed on the longissimus dorsi muscle from control and Nano VA treated groups. A total of 90 differentially expressed circRNAs (DECs), 805 differentially expressed lncRNAs (DELs), and 58 differentially expressed miRNAs (DEmiRs) were screened between the two groups. Among these, 53 DECs were upregulated and 38 were downregulated; 430 DELs were upregulated and 375 were downregulated; 33 DEmiRs were upregulated and 25 were downregulated. GO and KEGG enrichment analyses indicated that the DECs, DELs, and DEmiRs were significantly enriched in pathways including insulin signaling, mTOR signaling, cGMP-PKG signaling, FoxO signaling, retinol metabolism, MAPK signaling, and autophagy. Based on the competitive endogenous RNA regulatory mechanism, a circRNA/lncRNA-miRNA-mRNA network associated with Intramuscular fat deposition was constructed, comprising 9 mRNAs, 3 miRNAs, 50 lncRNAs, and 9 circRNAs, including circRNA2640/circRNA11556-ssc-miR-1296-5p-PIK3AP1, MSTRG.543.4/MSTRG.1281.3 -ssc-miR-17-3p-XIAP, MSTRG.7503.15/circRNA11514-ssc-miR-17-3p-ALDH1A1 and MSTRG.12101.2/ciRNA113-ssc-miR-885-3-GPAT4. These findings suggest that Nano VA likely regulates Intramuscular fat deposition through the above ceRNA network. Conclusions This study reveals that Nano VA promotes porcine Intramuscular fat deposition by modulating an ncRNA mediated ceRNA network, providing a theoretical basis for understanding the molecular mechanisms by which Nano VA improves pork quality.https://doi.org/10.1186/s12864-025-11898-yNano vitamin aIntramuscular fatCircRNAsLncRNAsMiRNAs |
| spellingShingle | Meng Li Wenrun Cai Ruotong Li Wenye Zhao Jiaxin Zhang Xiaoyin Rong Ting Zhang Yang Yang Bugao Li Xiaohong Guo Molecular mechanism of nano-vitamin A-mediated regulation of intramuscular fat deposition involving noncoding RNAs in pigs BMC Genomics Nano vitamin a Intramuscular fat CircRNAs LncRNAs MiRNAs |
| title | Molecular mechanism of nano-vitamin A-mediated regulation of intramuscular fat deposition involving noncoding RNAs in pigs |
| title_full | Molecular mechanism of nano-vitamin A-mediated regulation of intramuscular fat deposition involving noncoding RNAs in pigs |
| title_fullStr | Molecular mechanism of nano-vitamin A-mediated regulation of intramuscular fat deposition involving noncoding RNAs in pigs |
| title_full_unstemmed | Molecular mechanism of nano-vitamin A-mediated regulation of intramuscular fat deposition involving noncoding RNAs in pigs |
| title_short | Molecular mechanism of nano-vitamin A-mediated regulation of intramuscular fat deposition involving noncoding RNAs in pigs |
| title_sort | molecular mechanism of nano vitamin a mediated regulation of intramuscular fat deposition involving noncoding rnas in pigs |
| topic | Nano vitamin a Intramuscular fat CircRNAs LncRNAs MiRNAs |
| url | https://doi.org/10.1186/s12864-025-11898-y |
| work_keys_str_mv | AT mengli molecularmechanismofnanovitaminamediatedregulationofintramuscularfatdepositioninvolvingnoncodingrnasinpigs AT wenruncai molecularmechanismofnanovitaminamediatedregulationofintramuscularfatdepositioninvolvingnoncodingrnasinpigs AT ruotongli molecularmechanismofnanovitaminamediatedregulationofintramuscularfatdepositioninvolvingnoncodingrnasinpigs AT wenyezhao molecularmechanismofnanovitaminamediatedregulationofintramuscularfatdepositioninvolvingnoncodingrnasinpigs AT jiaxinzhang molecularmechanismofnanovitaminamediatedregulationofintramuscularfatdepositioninvolvingnoncodingrnasinpigs AT xiaoyinrong molecularmechanismofnanovitaminamediatedregulationofintramuscularfatdepositioninvolvingnoncodingrnasinpigs AT tingzhang molecularmechanismofnanovitaminamediatedregulationofintramuscularfatdepositioninvolvingnoncodingrnasinpigs AT yangyang molecularmechanismofnanovitaminamediatedregulationofintramuscularfatdepositioninvolvingnoncodingrnasinpigs AT bugaoli molecularmechanismofnanovitaminamediatedregulationofintramuscularfatdepositioninvolvingnoncodingrnasinpigs AT xiaohongguo molecularmechanismofnanovitaminamediatedregulationofintramuscularfatdepositioninvolvingnoncodingrnasinpigs |