mSphere of Influence: High-throughput screens to rapidly assign function to microbial genes

ABSTRACT Lori Huberman works in the field of fungal genetics, with an emphasis on investigating the genetic mechanisms fungi use to sense and respond to the nutrients and toxins in their environment. In this mSphere of Influence article, she reflects on how “Rapid quantification of mutant fitness in...

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Bibliographic Details
Main Author: Lori B. Huberman
Format: Article
Language:English
Published: American Society for Microbiology 2025-02-01
Series:mSphere
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Online Access:https://journals.asm.org/doi/10.1128/msphere.00800-24
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Summary:ABSTRACT Lori Huberman works in the field of fungal genetics, with an emphasis on investigating the genetic mechanisms fungi use to sense and respond to the nutrients and toxins in their environment. In this mSphere of Influence article, she reflects on how “Rapid quantification of mutant fitness in diverse bacteria by sequencing randomly bar-coded transposons” by K. M. Wetmore, M. N. Price, R. J. Waters, J. S. Lamson, et al. (mBio 6:e00306-15, 2015, https://doi.org/10.1128/mBio.00306-15) made an impact on her by establishing technologies that open realistic possibilities for developing high-throughput screening methods to correlate phenotype to genotype in diverse fungal species.
ISSN:2379-5042