Downstream metabolites of (+)-cis-12-oxo-phytodienoic acid function as noncanonical bioactive jasmonates in Arabidopsis thaliana

Abstract (+)-cis-12-oxo-phytodienoic acid (cis-OPDA) is a biosynthetic precursor of the plant hormone (+)−7-iso-Jasmonoyl-L-isoleucine (JA-Ile). It was proposed as an endogenous chemical signal independent of the JA-Ile receptor COI1-JAZ in Arabidopsis thaliana. The bioactive form of cis-OPDA that i...

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Main Authors: Minoru Ueda, Rina Saito, Yuho Nishizato, Tsumugi Kitajima, Nobuki Kato
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-61072-x
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author Minoru Ueda
Rina Saito
Yuho Nishizato
Tsumugi Kitajima
Nobuki Kato
author_facet Minoru Ueda
Rina Saito
Yuho Nishizato
Tsumugi Kitajima
Nobuki Kato
author_sort Minoru Ueda
collection DOAJ
description Abstract (+)-cis-12-oxo-phytodienoic acid (cis-OPDA) is a biosynthetic precursor of the plant hormone (+)−7-iso-Jasmonoyl-L-isoleucine (JA-Ile). It was proposed as an endogenous chemical signal independent of the JA-Ile receptor COI1-JAZ in Arabidopsis thaliana. The bioactive form of cis-OPDA that induces COI1-JAZ-independent gene expression remains unknown. Here we demonstrate that the downstream metabolites of cis-OPDA, especially tetranor-cis-OPDA (tn-cis-OPDA) and 7-iso-4,5-didehydro-JA (4,5-ddh-JA), upregulate the expression of the OPDA marker genes such as ZAT10 and ERF5. These downstream metabolites function independently of the JA-Ile-COI1-JAZ-MYCs canonical jasmonate signaling module, and their electrophilic α, β-unsaturated carbonyl functionalities are essential for their bioactivity. These results raise a possibility that the downstream metabolites of cis-OPDA would function as endogenous chemical signal in A. thaliana.
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spelling doaj-art-206abb297034410b87c50b0dcd4df6e92025-08-20T04:03:06ZengNature PortfolioNature Communications2041-17232025-07-0116111310.1038/s41467-025-61072-xDownstream metabolites of (+)-cis-12-oxo-phytodienoic acid function as noncanonical bioactive jasmonates in Arabidopsis thalianaMinoru Ueda0Rina Saito1Yuho Nishizato2Tsumugi Kitajima3Nobuki Kato4Department of Chemistry, Graduate School of Science, Tohoku UniversityDepartment of Molecular and Chemical Life Sciences, Graduate School of Life Sciences, Tohoku UniversityDepartment of Chemistry, Graduate School of Science, Tohoku UniversityDepartment of Molecular and Chemical Life Sciences, Graduate School of Life Sciences, Tohoku UniversityDepartment of Chemistry, Graduate School of Science, Tohoku UniversityAbstract (+)-cis-12-oxo-phytodienoic acid (cis-OPDA) is a biosynthetic precursor of the plant hormone (+)−7-iso-Jasmonoyl-L-isoleucine (JA-Ile). It was proposed as an endogenous chemical signal independent of the JA-Ile receptor COI1-JAZ in Arabidopsis thaliana. The bioactive form of cis-OPDA that induces COI1-JAZ-independent gene expression remains unknown. Here we demonstrate that the downstream metabolites of cis-OPDA, especially tetranor-cis-OPDA (tn-cis-OPDA) and 7-iso-4,5-didehydro-JA (4,5-ddh-JA), upregulate the expression of the OPDA marker genes such as ZAT10 and ERF5. These downstream metabolites function independently of the JA-Ile-COI1-JAZ-MYCs canonical jasmonate signaling module, and their electrophilic α, β-unsaturated carbonyl functionalities are essential for their bioactivity. These results raise a possibility that the downstream metabolites of cis-OPDA would function as endogenous chemical signal in A. thaliana.https://doi.org/10.1038/s41467-025-61072-x
spellingShingle Minoru Ueda
Rina Saito
Yuho Nishizato
Tsumugi Kitajima
Nobuki Kato
Downstream metabolites of (+)-cis-12-oxo-phytodienoic acid function as noncanonical bioactive jasmonates in Arabidopsis thaliana
Nature Communications
title Downstream metabolites of (+)-cis-12-oxo-phytodienoic acid function as noncanonical bioactive jasmonates in Arabidopsis thaliana
title_full Downstream metabolites of (+)-cis-12-oxo-phytodienoic acid function as noncanonical bioactive jasmonates in Arabidopsis thaliana
title_fullStr Downstream metabolites of (+)-cis-12-oxo-phytodienoic acid function as noncanonical bioactive jasmonates in Arabidopsis thaliana
title_full_unstemmed Downstream metabolites of (+)-cis-12-oxo-phytodienoic acid function as noncanonical bioactive jasmonates in Arabidopsis thaliana
title_short Downstream metabolites of (+)-cis-12-oxo-phytodienoic acid function as noncanonical bioactive jasmonates in Arabidopsis thaliana
title_sort downstream metabolites of cis 12 oxo phytodienoic acid function as noncanonical bioactive jasmonates in arabidopsis thaliana
url https://doi.org/10.1038/s41467-025-61072-x
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