Detection of Helicobacter pylori in stool specimens: comparative evaluation of nested PCR and antigen detection
Background: Efficacy of Helicobacter pylori stool antigen enzyme immunoassay (HpSA) and stool PCR was evaluated, before and after treatment, in a country with a high prevalence of H. pylori infection. Methodology: A total of 52 patients with dyspeptic symptoms were included in the study. Antral biop...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Journal of Infection in Developing Countries
2008-06-01
|
| Series: | Journal of Infection in Developing Countries |
| Subjects: | |
| Online Access: | https://jidc.org/index.php/journal/article/view/264 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850187334636535808 |
|---|---|
| author | Shrutkirti Mishra Varsha Singh GRKoteswar Rao Ashok Kumar Jain Vinod Kumar Dixit Anil Kumar Gulati Gopal Nath |
| author_facet | Shrutkirti Mishra Varsha Singh GRKoteswar Rao Ashok Kumar Jain Vinod Kumar Dixit Anil Kumar Gulati Gopal Nath |
| author_sort | Shrutkirti Mishra |
| collection | DOAJ |
| description | Background: Efficacy of Helicobacter pylori stool antigen enzyme immunoassay (HpSA) and stool PCR was evaluated, before and after treatment, in a country with a high prevalence of H. pylori infection.
Methodology: A total of 52 patients with dyspeptic symptoms were included in the study. Antral biopsy was collected during pre- and post-therapy periods for rapid urease test (RUT) and PCR. Similarly stool specimens for PCR and HpSA test were collected during both the periods from all 52 patients. Biopsy, PCR and RUT results together were considered the "gold standard."
Results: On the basis of gold standard tests, 40/52 patients were H. pylori positive. The sensitivity and specificity of HpSA test were 80% and 83.3% respectively in untreated patients. On the other hand, the sensitivity and specificity of stool PCR in untreated patients were 72.5% and 100% respectively. After eradication therapy, the results of both RUT and biopsy PCR were negative in 87.5% and positive in 12.5% of the patients. Although post treatment sensitivity of HpSA and stool PCR was equal (60%), specificity of HpSA and stool PCR were 68.6% and 97.1% respectively.
Conclusion: The H. pylori stool tests represent a non-invasive concept for diagnosis of infection. Both HpSA and stool PCR seem to be satisfactory tests for pre-eradication as well as assessment of infection. But stool PCR is a better indicator than HpSA test in the post-eradication assessment of infection. |
| format | Article |
| id | doaj-art-20674555eee94f67ad4f04fac4a8c0e9 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2008-06-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-20674555eee94f67ad4f04fac4a8c0e92025-08-20T02:16:07ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802008-06-0120310.3855/jidc.264Detection of Helicobacter pylori in stool specimens: comparative evaluation of nested PCR and antigen detectionShrutkirti Mishra0Varsha Singh1GRKoteswar Rao2Ashok Kumar Jain3Vinod Kumar Dixit4Anil Kumar Gulati5Gopal Nath6Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005Department of Gastroenterology, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005Department of Gastroenterology, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005Background: Efficacy of Helicobacter pylori stool antigen enzyme immunoassay (HpSA) and stool PCR was evaluated, before and after treatment, in a country with a high prevalence of H. pylori infection. Methodology: A total of 52 patients with dyspeptic symptoms were included in the study. Antral biopsy was collected during pre- and post-therapy periods for rapid urease test (RUT) and PCR. Similarly stool specimens for PCR and HpSA test were collected during both the periods from all 52 patients. Biopsy, PCR and RUT results together were considered the "gold standard." Results: On the basis of gold standard tests, 40/52 patients were H. pylori positive. The sensitivity and specificity of HpSA test were 80% and 83.3% respectively in untreated patients. On the other hand, the sensitivity and specificity of stool PCR in untreated patients were 72.5% and 100% respectively. After eradication therapy, the results of both RUT and biopsy PCR were negative in 87.5% and positive in 12.5% of the patients. Although post treatment sensitivity of HpSA and stool PCR was equal (60%), specificity of HpSA and stool PCR were 68.6% and 97.1% respectively. Conclusion: The H. pylori stool tests represent a non-invasive concept for diagnosis of infection. Both HpSA and stool PCR seem to be satisfactory tests for pre-eradication as well as assessment of infection. But stool PCR is a better indicator than HpSA test in the post-eradication assessment of infection.https://jidc.org/index.php/journal/article/view/264H. pyloriHpSAStool PCRBiopsy PCRRUThsp |
| spellingShingle | Shrutkirti Mishra Varsha Singh GRKoteswar Rao Ashok Kumar Jain Vinod Kumar Dixit Anil Kumar Gulati Gopal Nath Detection of Helicobacter pylori in stool specimens: comparative evaluation of nested PCR and antigen detection Journal of Infection in Developing Countries H. pylori HpSA Stool PCR Biopsy PCR RUT hsp |
| title | Detection of Helicobacter pylori in stool specimens: comparative evaluation of nested PCR and antigen detection |
| title_full | Detection of Helicobacter pylori in stool specimens: comparative evaluation of nested PCR and antigen detection |
| title_fullStr | Detection of Helicobacter pylori in stool specimens: comparative evaluation of nested PCR and antigen detection |
| title_full_unstemmed | Detection of Helicobacter pylori in stool specimens: comparative evaluation of nested PCR and antigen detection |
| title_short | Detection of Helicobacter pylori in stool specimens: comparative evaluation of nested PCR and antigen detection |
| title_sort | detection of helicobacter pylori in stool specimens comparative evaluation of nested pcr and antigen detection |
| topic | H. pylori HpSA Stool PCR Biopsy PCR RUT hsp |
| url | https://jidc.org/index.php/journal/article/view/264 |
| work_keys_str_mv | AT shrutkirtimishra detectionofhelicobacterpyloriinstoolspecimenscomparativeevaluationofnestedpcrandantigendetection AT varshasingh detectionofhelicobacterpyloriinstoolspecimenscomparativeevaluationofnestedpcrandantigendetection AT grkoteswarrao detectionofhelicobacterpyloriinstoolspecimenscomparativeevaluationofnestedpcrandantigendetection AT ashokkumarjain detectionofhelicobacterpyloriinstoolspecimenscomparativeevaluationofnestedpcrandantigendetection AT vinodkumardixit detectionofhelicobacterpyloriinstoolspecimenscomparativeevaluationofnestedpcrandantigendetection AT anilkumargulati detectionofhelicobacterpyloriinstoolspecimenscomparativeevaluationofnestedpcrandantigendetection AT gopalnath detectionofhelicobacterpyloriinstoolspecimenscomparativeevaluationofnestedpcrandantigendetection |