Induction of gonadal maturation of eel using PMSG, antidopamine, and estradiol-17β
ABSTRACT The study was aimed to induce gonadal maturation of eel Anguilla bicolor bicolor by hormonal treatment using pregnant mare serum gonadotropin (PMSG), antidopamine (AD), dan estradiol-17β (E2). The research used complete randomized design with five hormone combination treatments consisted...
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| Format: | Article |
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Asosiasi Sains Akuakultur Indonesia
2015-10-01
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| Series: | Jurnal Akuakultur Indonesia |
| Online Access: | https://journal.ipb.ac.id/index.php/jai/article/view/10351 |
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| author | Aprelia Martina Tomasoa Agus Oman Sudrajat Muhammad Zairin Junior |
| author_facet | Aprelia Martina Tomasoa Agus Oman Sudrajat Muhammad Zairin Junior |
| author_sort | Aprelia Martina Tomasoa |
| collection | DOAJ |
| description | ABSTRACT
The study was aimed to induce gonadal maturation of eel Anguilla bicolor bicolor by hormonal treatment using pregnant mare serum gonadotropin (PMSG), antidopamine (AD), dan estradiol-17β (E2). The research used complete randomized design with five hormone combination treatments consisted of PK (NaCl 0.95%) as control, P10A (PMSG 10 IU + AD 10 ppm), P20A (PMSG 20 IU + AD 10 ppm), P10BE (PMSG 10 IU + AD 10 ppm + E2 150 µg), and P20BE (PMSG 20 IU + AD 10 ppm + E2 150 µg), with three individual replications for each treatment. Hormonal induction was applied through intramuscular injection weekly during eight weeks at initial body weight of 200 g. The result showed that P10BE treatment has obtained highest level on E2 (0.43 ng/mL), FSH (2.68 mIU/mL) has increased in week-4 and level on T (1.2 ng/mL), LH (2.80 mIU/mL) has increased in week-8. P10BE has affected spermatogenesis and the increased of GSI (2.46%) in fourth and sixth week compared to PK (1.28%), P10A (1.58%), P20A (1.34%), and P20BE (2.12%). In conclusion, combination of PMSG, AD, and E2 hormones could stimulate the gonadal maturation of eel at the size of 200 g into male.
Keywords: Anguilla bicolor bicolor, gonadal growth, hormone, maturation
ABSTRAK
Penelitian ini dilakukan untuk menginduksi pematangan gonad ikan sidat Anguilla bicolor bicolor secara hormonal dengan menggunakan pregnant mare serum gonadothropin (PMSG), antidopamin (AD), dan estradiol-17β (E2). Metode penelitian ini menggunakan metode eksperimen rancangan acak lengkap dengan lima perlakuan kombinasi hormon, yaitu PK (larutan NaCl 0,95%) sebagai kontrol, P10A (PMSG 10 IU + AD 10 ppm), P20A (PMSG 20 IU + AD 10 ppm), P10BE (PMSG 10 IU + AD 10 ppm + E2 150 µg), dan P20BE (PMSG 10 IU+AD 10 ppm+E2 150 µg), dengan tiga ulangan individu pada masing-masing perlakuan. Induksi hormonal dilakukan dengan metode penyutikan secara intramuskuler setiap minggu selama delapan minggu dengan bobot ikan yang berukuran 200 g. Hasil penelitian menunjukkan bahwa analisis hormon pada perlakuan P10BE memberikan nilai tertinggi masing-masing; kadar E2 (0,43 ng/mL), kadar FSH (2,68 mIU/mL) meningkat di minggu keempat dan kadar T (1,2 ng/mL), kadar LH (2,80 mIU/mL) mengalami peningkatan pada minggu kedelapan. P10BE memberikan efek pada spermatogenesis dan peningkatan nilai GSI (2,46%) pada minggu keempat sampai keenam selama penyuntikkan dibandingkan dengan PK (1,28%), P10A (1,58%), P20A (1,34%) dan P20BE (2,12%). Dengan demikian, kombinasi hormon PMSG, AD, dan E2 dapat merangsang perkembangan dan mempercepat pematangan gonad ikan sidat ukuran 200 g menjadi jantan.
Kata kunci: Anguilla bicolor bicolor, pertumbuhan gonad, hormon, maturasi
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| format | Article |
| id | doaj-art-206276ab22684a06902328b233fe32c1 |
| institution | Kabale University |
| issn | 1412-5269 2354-6700 |
| language | English |
| publishDate | 2015-10-01 |
| publisher | Asosiasi Sains Akuakultur Indonesia |
| record_format | Article |
| series | Jurnal Akuakultur Indonesia |
| spelling | doaj-art-206276ab22684a06902328b233fe32c12025-01-24T17:58:44ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002015-10-0114210.19027/jai.14.112-1218522Induction of gonadal maturation of eel using PMSG, antidopamine, and estradiol-17βAprelia Martina Tomasoa0Agus Oman Sudrajat1Muhammad Zairin Junior2Departemen Budidaya Perairan Institut Pertanian BogorDepartemen Budidaya Perairan Institut Pertanian BogorDepartemen Budidaya Perairan Institut Pertanian BogorABSTRACT The study was aimed to induce gonadal maturation of eel Anguilla bicolor bicolor by hormonal treatment using pregnant mare serum gonadotropin (PMSG), antidopamine (AD), dan estradiol-17β (E2). The research used complete randomized design with five hormone combination treatments consisted of PK (NaCl 0.95%) as control, P10A (PMSG 10 IU + AD 10 ppm), P20A (PMSG 20 IU + AD 10 ppm), P10BE (PMSG 10 IU + AD 10 ppm + E2 150 µg), and P20BE (PMSG 20 IU + AD 10 ppm + E2 150 µg), with three individual replications for each treatment. Hormonal induction was applied through intramuscular injection weekly during eight weeks at initial body weight of 200 g. The result showed that P10BE treatment has obtained highest level on E2 (0.43 ng/mL), FSH (2.68 mIU/mL) has increased in week-4 and level on T (1.2 ng/mL), LH (2.80 mIU/mL) has increased in week-8. P10BE has affected spermatogenesis and the increased of GSI (2.46%) in fourth and sixth week compared to PK (1.28%), P10A (1.58%), P20A (1.34%), and P20BE (2.12%). In conclusion, combination of PMSG, AD, and E2 hormones could stimulate the gonadal maturation of eel at the size of 200 g into male. Keywords: Anguilla bicolor bicolor, gonadal growth, hormone, maturation ABSTRAK Penelitian ini dilakukan untuk menginduksi pematangan gonad ikan sidat Anguilla bicolor bicolor secara hormonal dengan menggunakan pregnant mare serum gonadothropin (PMSG), antidopamin (AD), dan estradiol-17β (E2). Metode penelitian ini menggunakan metode eksperimen rancangan acak lengkap dengan lima perlakuan kombinasi hormon, yaitu PK (larutan NaCl 0,95%) sebagai kontrol, P10A (PMSG 10 IU + AD 10 ppm), P20A (PMSG 20 IU + AD 10 ppm), P10BE (PMSG 10 IU + AD 10 ppm + E2 150 µg), dan P20BE (PMSG 10 IU+AD 10 ppm+E2 150 µg), dengan tiga ulangan individu pada masing-masing perlakuan. Induksi hormonal dilakukan dengan metode penyutikan secara intramuskuler setiap minggu selama delapan minggu dengan bobot ikan yang berukuran 200 g. Hasil penelitian menunjukkan bahwa analisis hormon pada perlakuan P10BE memberikan nilai tertinggi masing-masing; kadar E2 (0,43 ng/mL), kadar FSH (2,68 mIU/mL) meningkat di minggu keempat dan kadar T (1,2 ng/mL), kadar LH (2,80 mIU/mL) mengalami peningkatan pada minggu kedelapan. P10BE memberikan efek pada spermatogenesis dan peningkatan nilai GSI (2,46%) pada minggu keempat sampai keenam selama penyuntikkan dibandingkan dengan PK (1,28%), P10A (1,58%), P20A (1,34%) dan P20BE (2,12%). Dengan demikian, kombinasi hormon PMSG, AD, dan E2 dapat merangsang perkembangan dan mempercepat pematangan gonad ikan sidat ukuran 200 g menjadi jantan. Kata kunci: Anguilla bicolor bicolor, pertumbuhan gonad, hormon, maturasi https://journal.ipb.ac.id/index.php/jai/article/view/10351 |
| spellingShingle | Aprelia Martina Tomasoa Agus Oman Sudrajat Muhammad Zairin Junior Induction of gonadal maturation of eel using PMSG, antidopamine, and estradiol-17β Jurnal Akuakultur Indonesia |
| title | Induction of gonadal maturation of eel using PMSG, antidopamine, and estradiol-17β |
| title_full | Induction of gonadal maturation of eel using PMSG, antidopamine, and estradiol-17β |
| title_fullStr | Induction of gonadal maturation of eel using PMSG, antidopamine, and estradiol-17β |
| title_full_unstemmed | Induction of gonadal maturation of eel using PMSG, antidopamine, and estradiol-17β |
| title_short | Induction of gonadal maturation of eel using PMSG, antidopamine, and estradiol-17β |
| title_sort | induction of gonadal maturation of eel using pmsg antidopamine and estradiol 17β |
| url | https://journal.ipb.ac.id/index.php/jai/article/view/10351 |
| work_keys_str_mv | AT apreliamartinatomasoa inductionofgonadalmaturationofeelusingpmsgantidopamineandestradiol17b AT agusomansudrajat inductionofgonadalmaturationofeelusingpmsgantidopamineandestradiol17b AT muhammadzairinjunior inductionofgonadalmaturationofeelusingpmsgantidopamineandestradiol17b |