Design and Characterization of Decellularized Caprine Liver Matrix Constructs for Liver Tissue Engineering
Abstract This study focuses on developing and characterizing decellularized caprine liver scaffolds and their application in liver tissue engineering. Decellularization is achieved through chemical and enzymatic methods, effectively removing cellular components while preserving critical extracellula...
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2025-06-01
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| Series: | Macromolecular Materials and Engineering |
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| Online Access: | https://doi.org/10.1002/mame.202400451 |
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| author | Supriya Bhatt Jayanthi Krishnakumar Kondepudi Lakshmi Mounica Manasa Nune |
| author_facet | Supriya Bhatt Jayanthi Krishnakumar Kondepudi Lakshmi Mounica Manasa Nune |
| author_sort | Supriya Bhatt |
| collection | DOAJ |
| description | Abstract This study focuses on developing and characterizing decellularized caprine liver scaffolds and their application in liver tissue engineering. Decellularization is achieved through chemical and enzymatic methods, effectively removing cellular components while preserving critical extracellular matrix (ECM) elements such as collagen and glycosaminoglycans (GAGs), as confirmed by histological and biochemical analyses. The scaffolds are further processed into hydrogels by combining decellularized liver matrix (dLM) with chitosan (CH) and polyvinyl alcohol (PVA), optimized through freeze‐thaw (FT) cross‐linking. Rheological studies show shear‐thinning behavior and enhanced mechanical properties in the crosslinked dLM hydrogels, making them suitable for bioprinting applications. Scanning electron microscopy (SEM) reveals a porous structure favorable to cell adhesion, nutrient diffusion, and vascularization. Biocompatibility is confirmed through live/dead and MTT assays, demonstrating higher cell viability and proliferation on crosslinked scaffolds. HepG2 cells cultured on these scaffolds express hepatic‐specific markers, such as Albumin and Cytokeratin‐18, and exhibit functional capabilities, including urea metabolism and albumin synthesis, highlighting the scaffold's ability to support liver‐specific activities. Collectively, these findings demonstrate the potential of FT crosslinked dLM‐based hydrogels as promising candidates for liver tissue engineering, providing a biomimetic microenvironment that supports cellular functionality and promotes tissue regeneration. |
| format | Article |
| id | doaj-art-205cbd3e52d044f18f0f33d55f58313a |
| institution | DOAJ |
| issn | 1438-7492 1439-2054 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Macromolecular Materials and Engineering |
| spelling | doaj-art-205cbd3e52d044f18f0f33d55f58313a2025-08-20T03:21:23ZengWiley-VCHMacromolecular Materials and Engineering1438-74921439-20542025-06-013106n/an/a10.1002/mame.202400451Design and Characterization of Decellularized Caprine Liver Matrix Constructs for Liver Tissue EngineeringSupriya Bhatt0Jayanthi Krishnakumar1Kondepudi Lakshmi Mounica2Manasa Nune3Manipal Institute of Regenerative MedicineManipal Academy of Higher EducationManipal Karnataka 576104 IndiaManipal Institute of Regenerative MedicineManipal Academy of Higher EducationManipal Karnataka 576104 IndiaManipal Institute of Regenerative MedicineManipal Academy of Higher EducationManipal Karnataka 576104 IndiaManipal Institute of Regenerative MedicineManipal Academy of Higher EducationManipal Karnataka 576104 IndiaAbstract This study focuses on developing and characterizing decellularized caprine liver scaffolds and their application in liver tissue engineering. Decellularization is achieved through chemical and enzymatic methods, effectively removing cellular components while preserving critical extracellular matrix (ECM) elements such as collagen and glycosaminoglycans (GAGs), as confirmed by histological and biochemical analyses. The scaffolds are further processed into hydrogels by combining decellularized liver matrix (dLM) with chitosan (CH) and polyvinyl alcohol (PVA), optimized through freeze‐thaw (FT) cross‐linking. Rheological studies show shear‐thinning behavior and enhanced mechanical properties in the crosslinked dLM hydrogels, making them suitable for bioprinting applications. Scanning electron microscopy (SEM) reveals a porous structure favorable to cell adhesion, nutrient diffusion, and vascularization. Biocompatibility is confirmed through live/dead and MTT assays, demonstrating higher cell viability and proliferation on crosslinked scaffolds. HepG2 cells cultured on these scaffolds express hepatic‐specific markers, such as Albumin and Cytokeratin‐18, and exhibit functional capabilities, including urea metabolism and albumin synthesis, highlighting the scaffold's ability to support liver‐specific activities. Collectively, these findings demonstrate the potential of FT crosslinked dLM‐based hydrogels as promising candidates for liver tissue engineering, providing a biomimetic microenvironment that supports cellular functionality and promotes tissue regeneration.https://doi.org/10.1002/mame.2024004513D bioprintingcaprine liverdecellularizationextracellular matrixscaffoldstissue engineering |
| spellingShingle | Supriya Bhatt Jayanthi Krishnakumar Kondepudi Lakshmi Mounica Manasa Nune Design and Characterization of Decellularized Caprine Liver Matrix Constructs for Liver Tissue Engineering Macromolecular Materials and Engineering 3D bioprinting caprine liver decellularization extracellular matrix scaffolds tissue engineering |
| title | Design and Characterization of Decellularized Caprine Liver Matrix Constructs for Liver Tissue Engineering |
| title_full | Design and Characterization of Decellularized Caprine Liver Matrix Constructs for Liver Tissue Engineering |
| title_fullStr | Design and Characterization of Decellularized Caprine Liver Matrix Constructs for Liver Tissue Engineering |
| title_full_unstemmed | Design and Characterization of Decellularized Caprine Liver Matrix Constructs for Liver Tissue Engineering |
| title_short | Design and Characterization of Decellularized Caprine Liver Matrix Constructs for Liver Tissue Engineering |
| title_sort | design and characterization of decellularized caprine liver matrix constructs for liver tissue engineering |
| topic | 3D bioprinting caprine liver decellularization extracellular matrix scaffolds tissue engineering |
| url | https://doi.org/10.1002/mame.202400451 |
| work_keys_str_mv | AT supriyabhatt designandcharacterizationofdecellularizedcaprinelivermatrixconstructsforlivertissueengineering AT jayanthikrishnakumar designandcharacterizationofdecellularizedcaprinelivermatrixconstructsforlivertissueengineering AT kondepudilakshmimounica designandcharacterizationofdecellularizedcaprinelivermatrixconstructsforlivertissueengineering AT manasanune designandcharacterizationofdecellularizedcaprinelivermatrixconstructsforlivertissueengineering |