Ethyl Pyruvate Prevents Methyglyoxal-Induced Retinal Vascular Injury in Rats

Pyruvate is an endogenous antioxidant substance. The aim of this study was to investigate the protective effects of ethyl pyruvate (EP) on retinal vascular injury in diabetic retinopathy. To investigate the protective effect of EP on vascular cell apoptosis and blood-retinal barrier (BRB) breakage,...

Full description

Saved in:
Bibliographic Details
Main Authors: Junghyun Kim, Yun Mi Lee, Chan-Sik Kim, Eunjin Sohn, Kyuhyung Jo, So Dam Shin, Jin Sook Kim
Format: Article
Language:English
Published: Wiley 2013-01-01
Series:Journal of Diabetes Research
Online Access:http://dx.doi.org/10.1155/2013/460820
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850228391199899648
author Junghyun Kim
Yun Mi Lee
Chan-Sik Kim
Eunjin Sohn
Kyuhyung Jo
So Dam Shin
Jin Sook Kim
author_facet Junghyun Kim
Yun Mi Lee
Chan-Sik Kim
Eunjin Sohn
Kyuhyung Jo
So Dam Shin
Jin Sook Kim
author_sort Junghyun Kim
collection DOAJ
description Pyruvate is an endogenous antioxidant substance. The aim of this study was to investigate the protective effects of ethyl pyruvate (EP) on retinal vascular injury in diabetic retinopathy. To investigate the protective effect of EP on vascular cell apoptosis and blood-retinal barrier (BRB) breakage, we have used intravitreally methylglyoxal-(MGO-) injected rat eyes. Apoptosis of the retinal vascular cell that was stimulated by the intravitreal injection of MGO was evidently attenuated by the EP treatment. EP exerts inhibitory effect on MGO-induced vascular cell apoptosis by blocking oxidative injury. In addition, EP treatment prevented MGO-induced BRB breakage and the degradation of occludin, an important tight junction protein. These observations suggest that EP acts through an antioxidant mechanism to protect against oxidative stress-induced apoptosis in retinal vessels.
format Article
id doaj-art-2049753537eb4c0b9de940ff23f1cdfb
institution OA Journals
issn 2314-6745
2314-6753
language English
publishDate 2013-01-01
publisher Wiley
record_format Article
series Journal of Diabetes Research
spelling doaj-art-2049753537eb4c0b9de940ff23f1cdfb2025-08-20T02:04:33ZengWileyJournal of Diabetes Research2314-67452314-67532013-01-01201310.1155/2013/460820460820Ethyl Pyruvate Prevents Methyglyoxal-Induced Retinal Vascular Injury in RatsJunghyun Kim0Yun Mi Lee1Chan-Sik Kim2Eunjin Sohn3Kyuhyung Jo4So Dam Shin5Jin Sook Kim6Korean Medicine Based Herbal Drug Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine, 1672 Yuseongdaero, Yuseong-gu, Daejeon 305-811, Republic of KoreaKorean Medicine Based Herbal Drug Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine, 1672 Yuseongdaero, Yuseong-gu, Daejeon 305-811, Republic of KoreaKorean Medicine Based Herbal Drug Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine, 1672 Yuseongdaero, Yuseong-gu, Daejeon 305-811, Republic of KoreaKorean Medicine Based Herbal Drug Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine, 1672 Yuseongdaero, Yuseong-gu, Daejeon 305-811, Republic of KoreaKorean Medicine Based Herbal Drug Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine, 1672 Yuseongdaero, Yuseong-gu, Daejeon 305-811, Republic of KoreaKorean Medicine Based Herbal Drug Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine, 1672 Yuseongdaero, Yuseong-gu, Daejeon 305-811, Republic of KoreaKorean Medicine Based Herbal Drug Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine, 1672 Yuseongdaero, Yuseong-gu, Daejeon 305-811, Republic of KoreaPyruvate is an endogenous antioxidant substance. The aim of this study was to investigate the protective effects of ethyl pyruvate (EP) on retinal vascular injury in diabetic retinopathy. To investigate the protective effect of EP on vascular cell apoptosis and blood-retinal barrier (BRB) breakage, we have used intravitreally methylglyoxal-(MGO-) injected rat eyes. Apoptosis of the retinal vascular cell that was stimulated by the intravitreal injection of MGO was evidently attenuated by the EP treatment. EP exerts inhibitory effect on MGO-induced vascular cell apoptosis by blocking oxidative injury. In addition, EP treatment prevented MGO-induced BRB breakage and the degradation of occludin, an important tight junction protein. These observations suggest that EP acts through an antioxidant mechanism to protect against oxidative stress-induced apoptosis in retinal vessels.http://dx.doi.org/10.1155/2013/460820
spellingShingle Junghyun Kim
Yun Mi Lee
Chan-Sik Kim
Eunjin Sohn
Kyuhyung Jo
So Dam Shin
Jin Sook Kim
Ethyl Pyruvate Prevents Methyglyoxal-Induced Retinal Vascular Injury in Rats
Journal of Diabetes Research
title Ethyl Pyruvate Prevents Methyglyoxal-Induced Retinal Vascular Injury in Rats
title_full Ethyl Pyruvate Prevents Methyglyoxal-Induced Retinal Vascular Injury in Rats
title_fullStr Ethyl Pyruvate Prevents Methyglyoxal-Induced Retinal Vascular Injury in Rats
title_full_unstemmed Ethyl Pyruvate Prevents Methyglyoxal-Induced Retinal Vascular Injury in Rats
title_short Ethyl Pyruvate Prevents Methyglyoxal-Induced Retinal Vascular Injury in Rats
title_sort ethyl pyruvate prevents methyglyoxal induced retinal vascular injury in rats
url http://dx.doi.org/10.1155/2013/460820
work_keys_str_mv AT junghyunkim ethylpyruvatepreventsmethyglyoxalinducedretinalvascularinjuryinrats
AT yunmilee ethylpyruvatepreventsmethyglyoxalinducedretinalvascularinjuryinrats
AT chansikkim ethylpyruvatepreventsmethyglyoxalinducedretinalvascularinjuryinrats
AT eunjinsohn ethylpyruvatepreventsmethyglyoxalinducedretinalvascularinjuryinrats
AT kyuhyungjo ethylpyruvatepreventsmethyglyoxalinducedretinalvascularinjuryinrats
AT sodamshin ethylpyruvatepreventsmethyglyoxalinducedretinalvascularinjuryinrats
AT jinsookkim ethylpyruvatepreventsmethyglyoxalinducedretinalvascularinjuryinrats