Immune Reactions Against Elongation Factor 2 Kinase: Specific Pathogenesis of Gastric Ulcer from Helicobacter pylori Infection
Helicobacter pylori (H. pylori) infection is a definite causative factor for gastric ulcers (GUs). In the present study we detected a specific antigen of gastric epithelial cells (HGC-27) using cell ELISA, which was recognized by the sera of GU patients (n=20) but not in patients with chronic gastri...
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2009/850623 |
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| author | Kiyoshi Ayada Kenji Yokota Yoshiro Kawahara Yumiko Yamamoto Kazuyuki Hirai Tomoki Inaba Masahide Kita Hiroyuki Okada Kazuhide Yamamoto Keiji Oguma |
| author_facet | Kiyoshi Ayada Kenji Yokota Yoshiro Kawahara Yumiko Yamamoto Kazuyuki Hirai Tomoki Inaba Masahide Kita Hiroyuki Okada Kazuhide Yamamoto Keiji Oguma |
| author_sort | Kiyoshi Ayada |
| collection | DOAJ |
| description | Helicobacter pylori (H. pylori) infection is a definite causative factor for gastric ulcers (GUs). In the present study we detected a specific antigen of gastric epithelial cells (HGC-27) using cell ELISA, which was recognized by the sera of GU patients (n=20) but not in patients with chronic gastritis (CG; n=20) or in healthy volunteers (HC; n=10). This antigen was over-expressed by a stressful (heat-stressed) environment, and was identified as elongation factor 2 kinase (EF-2K) by western blotting. The GU patients' lymphocytes stimulated by H. pylori specifically disrupted heat-stressed HGC-27 cells in a cytotoxic assay. In flow cytometry, the effector cells (lymphocytes) from GU patients were significantly differentiated to T helper type 1 lymphocyte (Th1) and cytotoxic T lymphocyte (CTL) as opposed to those from CG patients. The target cells (HGC-27) expressed EF-2K and MHC-class I together with costimulatory molecules from heat stress. This antigen specific immune mechanism could have a prominent role in the pathogenesis of GU. |
| format | Article |
| id | doaj-art-204853fa5db44769ac99cdf524c358ed |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-204853fa5db44769ac99cdf524c358ed2025-02-03T05:58:11ZengWileyClinical and Developmental Immunology1740-25221740-25302009-01-01200910.1155/2009/850623850623Immune Reactions Against Elongation Factor 2 Kinase: Specific Pathogenesis of Gastric Ulcer from Helicobacter pylori InfectionKiyoshi Ayada0Kenji Yokota1Yoshiro Kawahara2Yumiko Yamamoto3Kazuyuki Hirai4Tomoki Inaba5Masahide Kita6Hiroyuki Okada7Kazuhide Yamamoto8Keiji Oguma9Department of Bacteriology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8558, JapanGraduate School of Health Science, Okayama University, 2-5-1 Shikata-cho, Okayama 700-8558, JapanDepartment of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8558, JapanDepartment of Bacteriology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8558, JapanDepartment of Bacteriology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8558, JapanDepartment of Internal Medicine, Kagawa Prefectural Central Hospital, 5-4-16 Ban-cho, Takamatsu, Kagawa 760-8557, JapanDepartment of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8558, JapanDepartment of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8558, JapanDepartment of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8558, JapanDepartment of Bacteriology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8558, JapanHelicobacter pylori (H. pylori) infection is a definite causative factor for gastric ulcers (GUs). In the present study we detected a specific antigen of gastric epithelial cells (HGC-27) using cell ELISA, which was recognized by the sera of GU patients (n=20) but not in patients with chronic gastritis (CG; n=20) or in healthy volunteers (HC; n=10). This antigen was over-expressed by a stressful (heat-stressed) environment, and was identified as elongation factor 2 kinase (EF-2K) by western blotting. The GU patients' lymphocytes stimulated by H. pylori specifically disrupted heat-stressed HGC-27 cells in a cytotoxic assay. In flow cytometry, the effector cells (lymphocytes) from GU patients were significantly differentiated to T helper type 1 lymphocyte (Th1) and cytotoxic T lymphocyte (CTL) as opposed to those from CG patients. The target cells (HGC-27) expressed EF-2K and MHC-class I together with costimulatory molecules from heat stress. This antigen specific immune mechanism could have a prominent role in the pathogenesis of GU.http://dx.doi.org/10.1155/2009/850623 |
| spellingShingle | Kiyoshi Ayada Kenji Yokota Yoshiro Kawahara Yumiko Yamamoto Kazuyuki Hirai Tomoki Inaba Masahide Kita Hiroyuki Okada Kazuhide Yamamoto Keiji Oguma Immune Reactions Against Elongation Factor 2 Kinase: Specific Pathogenesis of Gastric Ulcer from Helicobacter pylori Infection Clinical and Developmental Immunology |
| title | Immune Reactions Against Elongation Factor 2 Kinase: Specific Pathogenesis of Gastric Ulcer from Helicobacter pylori Infection |
| title_full | Immune Reactions Against Elongation Factor 2 Kinase: Specific Pathogenesis of Gastric Ulcer from Helicobacter pylori Infection |
| title_fullStr | Immune Reactions Against Elongation Factor 2 Kinase: Specific Pathogenesis of Gastric Ulcer from Helicobacter pylori Infection |
| title_full_unstemmed | Immune Reactions Against Elongation Factor 2 Kinase: Specific Pathogenesis of Gastric Ulcer from Helicobacter pylori Infection |
| title_short | Immune Reactions Against Elongation Factor 2 Kinase: Specific Pathogenesis of Gastric Ulcer from Helicobacter pylori Infection |
| title_sort | immune reactions against elongation factor 2 kinase specific pathogenesis of gastric ulcer from helicobacter pylori infection |
| url | http://dx.doi.org/10.1155/2009/850623 |
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