Nepalese White-Rot Fungus Ganoderma lucidum: A Potential Source of Ligninolytic Enzymes for Aromatic Dye Degradation
This study evaluates aromatic dyes degradation in solid and liquid media and the ligninolytic enzymes production by the white-rot fungus Ganoderma lucidum. The results demonstrated that G. lucidum degraded Congo red (CRD) and methylene blue (MBD) more rapidly than malachite green (MGD) (p < 0.00...
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Biotechnology Society of Nepal
2025-07-01
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| Series: | Nepal Journal of Biotechnology |
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| Online Access: | https://nepjb.com/index.php/NJB/article/view/385 |
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| author | Sandesh Gaudel Anu Kumal Indra Bahadur Chhetri Sunil Tiwari Ishor Thapa |
| author_facet | Sandesh Gaudel Anu Kumal Indra Bahadur Chhetri Sunil Tiwari Ishor Thapa |
| author_sort | Sandesh Gaudel |
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This study evaluates aromatic dyes degradation in solid and liquid media and the ligninolytic enzymes production by the white-rot fungus Ganoderma lucidum. The results demonstrated that G. lucidum degraded Congo red (CRD) and methylene blue (MBD) more rapidly than malachite green (MGD) (p < 0.0001). In the solid medium containing CRD and MBD, the fungus showed a high decolorization index, while its mycelial growth remained unaffected by the presence of dye. Similarly, in liquid media, G. lucidum effectively decolorized CRD and MBD. Similarly, the fungus was cultured in naphthalene supplemented liquid media to assess the ligninolytic enzyme production. Laccase activity peaked at 8 days (985 U L-1), while lignin peroxidase reached maximum activity at 14 days (1192 U L-1), followed by a decline in both enzymes. These results suggest the potential of G. lucidum for selective bioremediation of dye-contaminated environments.
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| format | Article |
| id | doaj-art-203d1d7ad84c4d47baab2f50657c035d |
| institution | Kabale University |
| issn | 2091-1130 2467-9313 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Biotechnology Society of Nepal |
| record_format | Article |
| series | Nepal Journal of Biotechnology |
| spelling | doaj-art-203d1d7ad84c4d47baab2f50657c035d2025-08-20T03:36:31ZengBiotechnology Society of NepalNepal Journal of Biotechnology2091-11302467-93132025-07-0113110.54796/njb.v13i1.385Nepalese White-Rot Fungus Ganoderma lucidum: A Potential Source of Ligninolytic Enzymes for Aromatic Dye DegradationSandesh Gaudel0https://orcid.org/0009-0006-4497-7288Anu Kumal1https://orcid.org/0009-0002-7622-808XIndra Bahadur Chhetri2https://orcid.org/0009-0001-4856-9816Sunil Tiwari3https://orcid.org/0000-0002-2967-838XIshor Thapa4https://orcid.org/0000-0002-9874-4563Department of Biotechnology, School of Science, Kathmandu UniversityDepartment of Biotechnology, SANN International College, Purbanchal UniversityCollege of Natural Resources Management, Agriculture and Forestry UniversityDepartment of Biotechnology, SANN International College, Purbanchal UniversityDepartment of Biotechnology, SANN International College, Purbanchal University This study evaluates aromatic dyes degradation in solid and liquid media and the ligninolytic enzymes production by the white-rot fungus Ganoderma lucidum. The results demonstrated that G. lucidum degraded Congo red (CRD) and methylene blue (MBD) more rapidly than malachite green (MGD) (p < 0.0001). In the solid medium containing CRD and MBD, the fungus showed a high decolorization index, while its mycelial growth remained unaffected by the presence of dye. Similarly, in liquid media, G. lucidum effectively decolorized CRD and MBD. Similarly, the fungus was cultured in naphthalene supplemented liquid media to assess the ligninolytic enzyme production. Laccase activity peaked at 8 days (985 U L-1), while lignin peroxidase reached maximum activity at 14 days (1192 U L-1), followed by a decline in both enzymes. These results suggest the potential of G. lucidum for selective bioremediation of dye-contaminated environments. https://nepjb.com/index.php/NJB/article/view/385DecolorizationAromatic dyesGanoderma lucidumEnzyme activitiesBiodegradationFungal bioremediation |
| spellingShingle | Sandesh Gaudel Anu Kumal Indra Bahadur Chhetri Sunil Tiwari Ishor Thapa Nepalese White-Rot Fungus Ganoderma lucidum: A Potential Source of Ligninolytic Enzymes for Aromatic Dye Degradation Nepal Journal of Biotechnology Decolorization Aromatic dyes Ganoderma lucidum Enzyme activities Biodegradation Fungal bioremediation |
| title | Nepalese White-Rot Fungus Ganoderma lucidum: A Potential Source of Ligninolytic Enzymes for Aromatic Dye Degradation |
| title_full | Nepalese White-Rot Fungus Ganoderma lucidum: A Potential Source of Ligninolytic Enzymes for Aromatic Dye Degradation |
| title_fullStr | Nepalese White-Rot Fungus Ganoderma lucidum: A Potential Source of Ligninolytic Enzymes for Aromatic Dye Degradation |
| title_full_unstemmed | Nepalese White-Rot Fungus Ganoderma lucidum: A Potential Source of Ligninolytic Enzymes for Aromatic Dye Degradation |
| title_short | Nepalese White-Rot Fungus Ganoderma lucidum: A Potential Source of Ligninolytic Enzymes for Aromatic Dye Degradation |
| title_sort | nepalese white rot fungus ganoderma lucidum a potential source of ligninolytic enzymes for aromatic dye degradation |
| topic | Decolorization Aromatic dyes Ganoderma lucidum Enzyme activities Biodegradation Fungal bioremediation |
| url | https://nepjb.com/index.php/NJB/article/view/385 |
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