Emergence of invasive Streptococcus dysgalactiae subsp. equisimilis in Spain (2012-2022): genomic insights and clinical correlations
Objectives: An increase in Streptococcus dysgalactiae subsp. equisimilis (SDSE) infections has been documented worldwide. This study aims to analyze invasive disease caused by SDSE (iSDSE) in adults over an 11-year period in Spain. Methods: We conducted a retrospective, laboratory-based study of iSD...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-04-01
|
| Series: | International Journal of Infectious Diseases |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971225000025 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832576530870435840 |
|---|---|
| author | Guillem López de Egea Aida González-Díaz Randall J Olsen Gérard Guédon Dàmaris Berbel Immaculada Grau Jordi Càmara Lucía Saiz-Escobedo Sara Calvo-Silveria Irene Cadenas-Jiménez José María Marimón Emilia Cercenado Antonio Casabella Sara Martí M. Ángeles Domínguez Nathalie Leblond-Bourget James M Musser Carmen Ardanuy |
| author_facet | Guillem López de Egea Aida González-Díaz Randall J Olsen Gérard Guédon Dàmaris Berbel Immaculada Grau Jordi Càmara Lucía Saiz-Escobedo Sara Calvo-Silveria Irene Cadenas-Jiménez José María Marimón Emilia Cercenado Antonio Casabella Sara Martí M. Ángeles Domínguez Nathalie Leblond-Bourget James M Musser Carmen Ardanuy |
| author_sort | Guillem López de Egea |
| collection | DOAJ |
| description | Objectives: An increase in Streptococcus dysgalactiae subsp. equisimilis (SDSE) infections has been documented worldwide. This study aims to analyze invasive disease caused by SDSE (iSDSE) in adults over an 11-year period in Spain. Methods: We conducted a retrospective, laboratory-based study of iSDSE detected at Hospital Universitari de Bellvitge (HUB) from 2012 to 2022 (n = 89) and isolates collected in three Spanish hospitals in 2018 (n = 22). Clinical data from HUB were collected. Isolates were tested for antimicrobial susceptibility (European Committee on Antimicrobial Susceptibility Testing 2023), subjected to whole genome sequencing and analyzed for mobile genetic elements (MGEs). A mouse model was used to analyze virulence. Results: iSDSE episodes at HUB occurred predominantly in older patients with comorbidities (particularly, diabetes, chronic heart disease, and malignancies). Whole genome sequencing revealed a high genetic diversity, with the most common lineages being CC15, CC17, and CC20. Various virulence factors, including the superantigen speG, were identified. Macrolides, lincosamides, and tetracyclines exhibited the highest resistance rates (>27%) and changed over time, linked to multiple MGEs. The mouse model highlighted the virulence of the CC20-stG62647 lineage, but these results were discordant with clinical data. Conclusion: iSDSE incidence is increasing and associated with older patients with comorbidities. Genetically, SDSE is diverse with a high capacity to integrate MGEs carrying resistance determinants. Mouse model studies showed the enhanced virulence of the CC20-stG62647 lineage. These findings underscore the need for ongoing surveillance of this emerging pathogen. |
| format | Article |
| id | doaj-art-202533aca3de4993b6b80341be1187ea |
| institution | Kabale University |
| issn | 1201-9712 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj-art-202533aca3de4993b6b80341be1187ea2025-01-31T05:10:12ZengElsevierInternational Journal of Infectious Diseases1201-97122025-04-01153107778Emergence of invasive Streptococcus dysgalactiae subsp. equisimilis in Spain (2012-2022): genomic insights and clinical correlationsGuillem López de Egea0Aida González-Díaz1Randall J Olsen2Gérard Guédon3Dàmaris Berbel4Immaculada Grau5Jordi Càmara6Lucía Saiz-Escobedo7Sara Calvo-Silveria8Irene Cadenas-Jiménez9José María Marimón10Emilia Cercenado11Antonio Casabella12Sara Martí13M. Ángeles Domínguez14Nathalie Leblond-Bourget15James M Musser16Carmen Ardanuy17Microbiology Department, Hospital Universitari Bellvitge, IDIBELL-UB, L'Hospitalet de Llobregat, Spain; Research Network for Respiratory Diseases (CIBERES), ISCIII, Madrid, Spain; Department of Pathology and Experimental Therapeutics, School of Medicine and Health Sciences, University of Barcelona, Barcelona, SpainMicrobiology Department, Hospital Universitari Bellvitge, IDIBELL-UB, L'Hospitalet de Llobregat, Spain; Research Network for Respiratory Diseases (CIBERES), ISCIII, Madrid, SpainLaboratory of Molecular and Translational Human Infectious Disease Research, Center for Infectious Diseases, Department of Pathology and Genomic Medicine, Houston Methodist Research Institute and Houston Methodist Hospital, Houston, USAUniversité de Lorraine, INRAE, DynAMic, Nancy, FranceMicrobiology Department, Hospital Universitari Bellvitge, IDIBELL-UB, L'Hospitalet de Llobregat, Spain; Research Network for Respiratory Diseases (CIBERES), ISCIII, Madrid, SpainResearch Network for Respiratory Diseases (CIBERES), ISCIII, Madrid, Spain; Infectious Diseases Department, Hospital Universitari Bellvitge, IDIBELL-UB, L'Hospitalet de Llobregat, SpainMicrobiology Department, Hospital Universitari Bellvitge, IDIBELL-UB, L'Hospitalet de Llobregat, Spain; Research Network for Respiratory Diseases (CIBERES), ISCIII, Madrid, SpainMicrobiology Department, Hospital Universitari Bellvitge, IDIBELL-UB, L'Hospitalet de Llobregat, SpainMicrobiology Department, Hospital Universitari Bellvitge, IDIBELL-UB, L'Hospitalet de Llobregat, Spain; Research Network for Respiratory Diseases (CIBERES), ISCIII, Madrid, SpainMicrobiology Department, Hospital Universitari Bellvitge, IDIBELL-UB, L'Hospitalet de Llobregat, Spain; Research Network for Respiratory Diseases (CIBERES), ISCIII, Madrid, SpainBiodonostia, Infectious Diseases Area, Respiratory Infection and Antimicrobial Resistance Group, Osakidetza Basque Health Service, Donostialdea Integrated Health Organization, Microbiology Department, San Sebastián, SpainResearch Network for Respiratory Diseases (CIBERES), ISCIII, Madrid, Spain; Clinical Microbiology and Infectious Disease Department, Hospital General Universitario Gregorio Marañón, Madrid, SpainMicrobiology Department, Parc Taulí Hospital Universitari, Institut d'Investigació i Innovació Parc Taulí (I3PT-CERCA), Universitat Autònoma de Barcelona, Sabadell, SpainMicrobiology Department, Hospital Universitari Bellvitge, IDIBELL-UB, L'Hospitalet de Llobregat, Spain; Research Network for Respiratory Diseases (CIBERES), ISCIII, Madrid, SpainMicrobiology Department, Hospital Universitari Bellvitge, IDIBELL-UB, L'Hospitalet de Llobregat, Spain; Department of Pathology and Experimental Therapeutics, School of Medicine and Health Sciences, University of Barcelona, Barcelona, Spain; Research Network for Infectious Diseases (CIBERINFEC), ISCIII, Madrid, SpainUniversité de Lorraine, INRAE, DynAMic, Nancy, FranceLaboratory of Molecular and Translational Human Infectious Disease Research, Center for Infectious Diseases, Department of Pathology and Genomic Medicine, Houston Methodist Research Institute and Houston Methodist Hospital, Houston, USAMicrobiology Department, Hospital Universitari Bellvitge, IDIBELL-UB, L'Hospitalet de Llobregat, Spain; Research Network for Respiratory Diseases (CIBERES), ISCIII, Madrid, Spain; Department of Pathology and Experimental Therapeutics, School of Medicine and Health Sciences, University of Barcelona, Barcelona, Spain; Corresponding author. Tel.: +34 93 260 79 30; fax: +34 93 260 75 47.Objectives: An increase in Streptococcus dysgalactiae subsp. equisimilis (SDSE) infections has been documented worldwide. This study aims to analyze invasive disease caused by SDSE (iSDSE) in adults over an 11-year period in Spain. Methods: We conducted a retrospective, laboratory-based study of iSDSE detected at Hospital Universitari de Bellvitge (HUB) from 2012 to 2022 (n = 89) and isolates collected in three Spanish hospitals in 2018 (n = 22). Clinical data from HUB were collected. Isolates were tested for antimicrobial susceptibility (European Committee on Antimicrobial Susceptibility Testing 2023), subjected to whole genome sequencing and analyzed for mobile genetic elements (MGEs). A mouse model was used to analyze virulence. Results: iSDSE episodes at HUB occurred predominantly in older patients with comorbidities (particularly, diabetes, chronic heart disease, and malignancies). Whole genome sequencing revealed a high genetic diversity, with the most common lineages being CC15, CC17, and CC20. Various virulence factors, including the superantigen speG, were identified. Macrolides, lincosamides, and tetracyclines exhibited the highest resistance rates (>27%) and changed over time, linked to multiple MGEs. The mouse model highlighted the virulence of the CC20-stG62647 lineage, but these results were discordant with clinical data. Conclusion: iSDSE incidence is increasing and associated with older patients with comorbidities. Genetically, SDSE is diverse with a high capacity to integrate MGEs carrying resistance determinants. Mouse model studies showed the enhanced virulence of the CC20-stG62647 lineage. These findings underscore the need for ongoing surveillance of this emerging pathogen.http://www.sciencedirect.com/science/article/pii/S1201971225000025Streptococcus dysgalactiaeSDSEVirulenceWhole genome sequencingHumansMice |
| spellingShingle | Guillem López de Egea Aida González-Díaz Randall J Olsen Gérard Guédon Dàmaris Berbel Immaculada Grau Jordi Càmara Lucía Saiz-Escobedo Sara Calvo-Silveria Irene Cadenas-Jiménez José María Marimón Emilia Cercenado Antonio Casabella Sara Martí M. Ángeles Domínguez Nathalie Leblond-Bourget James M Musser Carmen Ardanuy Emergence of invasive Streptococcus dysgalactiae subsp. equisimilis in Spain (2012-2022): genomic insights and clinical correlations International Journal of Infectious Diseases Streptococcus dysgalactiae SDSE Virulence Whole genome sequencing Humans Mice |
| title | Emergence of invasive Streptococcus dysgalactiae subsp. equisimilis in Spain (2012-2022): genomic insights and clinical correlations |
| title_full | Emergence of invasive Streptococcus dysgalactiae subsp. equisimilis in Spain (2012-2022): genomic insights and clinical correlations |
| title_fullStr | Emergence of invasive Streptococcus dysgalactiae subsp. equisimilis in Spain (2012-2022): genomic insights and clinical correlations |
| title_full_unstemmed | Emergence of invasive Streptococcus dysgalactiae subsp. equisimilis in Spain (2012-2022): genomic insights and clinical correlations |
| title_short | Emergence of invasive Streptococcus dysgalactiae subsp. equisimilis in Spain (2012-2022): genomic insights and clinical correlations |
| title_sort | emergence of invasive streptococcus dysgalactiae subsp equisimilis in spain 2012 2022 genomic insights and clinical correlations |
| topic | Streptococcus dysgalactiae SDSE Virulence Whole genome sequencing Humans Mice |
| url | http://www.sciencedirect.com/science/article/pii/S1201971225000025 |
| work_keys_str_mv | AT guillemlopezdeegea emergenceofinvasivestreptococcusdysgalactiaesubspequisimilisinspain20122022genomicinsightsandclinicalcorrelations AT aidagonzalezdiaz emergenceofinvasivestreptococcusdysgalactiaesubspequisimilisinspain20122022genomicinsightsandclinicalcorrelations AT randalljolsen emergenceofinvasivestreptococcusdysgalactiaesubspequisimilisinspain20122022genomicinsightsandclinicalcorrelations AT gerardguedon emergenceofinvasivestreptococcusdysgalactiaesubspequisimilisinspain20122022genomicinsightsandclinicalcorrelations AT damarisberbel emergenceofinvasivestreptococcusdysgalactiaesubspequisimilisinspain20122022genomicinsightsandclinicalcorrelations AT immaculadagrau emergenceofinvasivestreptococcusdysgalactiaesubspequisimilisinspain20122022genomicinsightsandclinicalcorrelations AT jordicamara emergenceofinvasivestreptococcusdysgalactiaesubspequisimilisinspain20122022genomicinsightsandclinicalcorrelations AT luciasaizescobedo emergenceofinvasivestreptococcusdysgalactiaesubspequisimilisinspain20122022genomicinsightsandclinicalcorrelations AT saracalvosilveria emergenceofinvasivestreptococcusdysgalactiaesubspequisimilisinspain20122022genomicinsightsandclinicalcorrelations AT irenecadenasjimenez emergenceofinvasivestreptococcusdysgalactiaesubspequisimilisinspain20122022genomicinsightsandclinicalcorrelations AT josemariamarimon emergenceofinvasivestreptococcusdysgalactiaesubspequisimilisinspain20122022genomicinsightsandclinicalcorrelations AT emiliacercenado emergenceofinvasivestreptococcusdysgalactiaesubspequisimilisinspain20122022genomicinsightsandclinicalcorrelations AT antoniocasabella emergenceofinvasivestreptococcusdysgalactiaesubspequisimilisinspain20122022genomicinsightsandclinicalcorrelations AT saramarti emergenceofinvasivestreptococcusdysgalactiaesubspequisimilisinspain20122022genomicinsightsandclinicalcorrelations AT mangelesdominguez emergenceofinvasivestreptococcusdysgalactiaesubspequisimilisinspain20122022genomicinsightsandclinicalcorrelations AT nathalieleblondbourget emergenceofinvasivestreptococcusdysgalactiaesubspequisimilisinspain20122022genomicinsightsandclinicalcorrelations AT jamesmmusser emergenceofinvasivestreptococcusdysgalactiaesubspequisimilisinspain20122022genomicinsightsandclinicalcorrelations AT carmenardanuy emergenceofinvasivestreptococcusdysgalactiaesubspequisimilisinspain20122022genomicinsightsandclinicalcorrelations |