Optimization of Spectrophotometric and Fluorometric Assays Using Alternative Substrates for the High-Throughput Screening of Lipase Activity
The effects of reaction conditions on the spectrophotometric and fluorometric assays using alternative substrates (p-nitrophenyl palmitate and 4-methylumbelliferyl oleate) were investigated to optimize them for the high-throughput screening of lipase activity from agricultural products. Four model l...
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Main Authors: | Jun-Young Park, Jisu Ha, Yoonseok Choi, Pahn-Shick Chang, Kyung-Min Park |
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Format: | Article |
Language: | English |
Published: |
Wiley
2021-01-01
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Series: | Journal of Chemistry |
Online Access: | http://dx.doi.org/10.1155/2021/3688124 |
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