Optimization of Spectrophotometric and Fluorometric Assays Using Alternative Substrates for the High-Throughput Screening of Lipase Activity

The effects of reaction conditions on the spectrophotometric and fluorometric assays using alternative substrates (p-nitrophenyl palmitate and 4-methylumbelliferyl oleate) were investigated to optimize them for the high-throughput screening of lipase activity from agricultural products. Four model l...

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Bibliographic Details
Main Authors: Jun-Young Park, Jisu Ha, Yoonseok Choi, Pahn-Shick Chang, Kyung-Min Park
Format: Article
Language:English
Published: Wiley 2021-01-01
Series:Journal of Chemistry
Online Access:http://dx.doi.org/10.1155/2021/3688124
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