The effect of vitamin A and glutamine on methotrexate induced hepatotoxicity in rats
Background: Methotrexate is one of the most effective and commonly used medicines to treat various forms of arthritis and other rheumatic conditions. It is known as a disease-modifying ant rheumatic drug (DMARD) because it not only decreases the pain and swelling of arthritis but also can reduce da...
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University of Kufa, Faculty of Nursing
2012-08-01
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| Series: | Kufa Journal for Nursing Sciences |
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| Online Access: | https://journal.uokufa.edu.iq/index.php/kjns/article/view/3134 |
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| author | Saadia S. M. AL–Zeiny Bassam A. AL_Shimmary Shatha M. AL- safi Najah R. Hadi Ahmed A. AL-Azam Asaad A. AL-janabi |
| author_facet | Saadia S. M. AL–Zeiny Bassam A. AL_Shimmary Shatha M. AL- safi Najah R. Hadi Ahmed A. AL-Azam Asaad A. AL-janabi |
| author_sort | Saadia S. M. AL–Zeiny |
| collection | DOAJ |
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Background: Methotrexate is one of the most effective and commonly used medicines to treat various forms of arthritis and other rheumatic conditions. It is known as a disease-modifying ant rheumatic drug (DMARD) because it not only decreases the pain and swelling of arthritis but also can reduce damage to joints and the risks of long-term disability.
Methodology: Twenty eight male rats, were taken of the research center in the College of Medicine at the University of Kufa. These animals were equally divided into four groups, Each of 7 rats, the first group received distilled water added equivalent fluid (corn oil, NaOH and citric acid) and served as control. The second group received methotrexate at dose of 2.5 mg/BW orally twice daily by gavage, the third group received methotrexate and vitamin A at doses of 2.5 mg /BW + 50000 IU respectively orally twice daily by gavage and the forth group received methotrexate and glutamine at dose 2.5 mg/BW + 500 mg /BW twice daily respectively. All groups dosing for period 30 days. At the end of the experiment, all rats were anesthetized using ether and drawn blood 4ml of heart using a 23-gauge needle syringe then killed and take piece of liver and placed in formalin 10 % for histopath examination. The blood placed in centrifuge 3000/minute for 10 minutes to obtain serum for analyzing liver function test (alkaline phosphatase, aspartate amino transferees (AST) and alanine amino transfers (ALT) measurements and bilirubin).
Result: In animal treatment with methotrexate only and those treated with MTX and Vitamin A and glutamine there was no significant difference in serum ALT, serum AST and Bilirubin when compared with control group but ALP elevated in MTX with vitamin A treated rats and no significant difference in MTX and MTX+ glutamine treated groups but showed amyloid changes liver and congestion in methotrexate treatment rat compared with control and no damage in liver of MTX with vitamin A and glutamine treated groups.
Conclusion: liver enzymes could be normal despite histopath change in methotrexate treated animals
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| format | Article |
| id | doaj-art-201d3f1153c44bd3a09ff159de71441a |
| institution | OA Journals |
| issn | 2223-4055 2521-8638 |
| language | English |
| publishDate | 2012-08-01 |
| publisher | University of Kufa, Faculty of Nursing |
| record_format | Article |
| series | Kufa Journal for Nursing Sciences |
| spelling | doaj-art-201d3f1153c44bd3a09ff159de71441a2025-08-20T02:12:54ZengUniversity of Kufa, Faculty of NursingKufa Journal for Nursing Sciences2223-40552521-86382012-08-012210.36321/kjns.vi20122.3134The effect of vitamin A and glutamine on methotrexate induced hepatotoxicity in ratsSaadia S. M. AL–Zeiny0Bassam A. AL_Shimmary1Shatha M. AL- safi2Najah R. Hadi3Ahmed A. AL-Azam4Asaad A. AL-janabi5University of KufaUniversity of KufaUniversity of KufaUniversity of KufaUniversity of KufaUniversity of Kufa Background: Methotrexate is one of the most effective and commonly used medicines to treat various forms of arthritis and other rheumatic conditions. It is known as a disease-modifying ant rheumatic drug (DMARD) because it not only decreases the pain and swelling of arthritis but also can reduce damage to joints and the risks of long-term disability. Methodology: Twenty eight male rats, were taken of the research center in the College of Medicine at the University of Kufa. These animals were equally divided into four groups, Each of 7 rats, the first group received distilled water added equivalent fluid (corn oil, NaOH and citric acid) and served as control. The second group received methotrexate at dose of 2.5 mg/BW orally twice daily by gavage, the third group received methotrexate and vitamin A at doses of 2.5 mg /BW + 50000 IU respectively orally twice daily by gavage and the forth group received methotrexate and glutamine at dose 2.5 mg/BW + 500 mg /BW twice daily respectively. All groups dosing for period 30 days. At the end of the experiment, all rats were anesthetized using ether and drawn blood 4ml of heart using a 23-gauge needle syringe then killed and take piece of liver and placed in formalin 10 % for histopath examination. The blood placed in centrifuge 3000/minute for 10 minutes to obtain serum for analyzing liver function test (alkaline phosphatase, aspartate amino transferees (AST) and alanine amino transfers (ALT) measurements and bilirubin). Result: In animal treatment with methotrexate only and those treated with MTX and Vitamin A and glutamine there was no significant difference in serum ALT, serum AST and Bilirubin when compared with control group but ALP elevated in MTX with vitamin A treated rats and no significant difference in MTX and MTX+ glutamine treated groups but showed amyloid changes liver and congestion in methotrexate treatment rat compared with control and no damage in liver of MTX with vitamin A and glutamine treated groups. Conclusion: liver enzymes could be normal despite histopath change in methotrexate treated animals https://journal.uokufa.edu.iq/index.php/kjns/article/view/3134vitamine Aglutaminemethotrexate induced hepatotoxicity |
| spellingShingle | Saadia S. M. AL–Zeiny Bassam A. AL_Shimmary Shatha M. AL- safi Najah R. Hadi Ahmed A. AL-Azam Asaad A. AL-janabi The effect of vitamin A and glutamine on methotrexate induced hepatotoxicity in rats Kufa Journal for Nursing Sciences vitamine A glutamine methotrexate induced hepatotoxicity |
| title | The effect of vitamin A and glutamine on methotrexate induced hepatotoxicity in rats |
| title_full | The effect of vitamin A and glutamine on methotrexate induced hepatotoxicity in rats |
| title_fullStr | The effect of vitamin A and glutamine on methotrexate induced hepatotoxicity in rats |
| title_full_unstemmed | The effect of vitamin A and glutamine on methotrexate induced hepatotoxicity in rats |
| title_short | The effect of vitamin A and glutamine on methotrexate induced hepatotoxicity in rats |
| title_sort | effect of vitamin a and glutamine on methotrexate induced hepatotoxicity in rats |
| topic | vitamine A glutamine methotrexate induced hepatotoxicity |
| url | https://journal.uokufa.edu.iq/index.php/kjns/article/view/3134 |
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