A ferroptosis-related risk model for non-coding RNA AC002331.1 in colon cancer: construction via competing endogenous RNA network analysis

A ferroptosis-related risk model for non-coding RNA AC002331.1 in colon cancer: construction via competing endogenous RNA network analysis

Abstract Background Colon cancer, a globally prevalent malignancy with high mortality, involves lncRNA regulation, ferroptosis pathway abnormalities, and gut microbiota dysbiosis. Ferroptosis-related gene models may aid prognostic evaluation, while microbiota metabolites modulate ferroptosis in cont...

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Bibliographic Details
Main Authors: Jiahui Liu, Chenning Zhang, Bao Ma, Jie Qi, Xinghai Zhang, Hengli Zhao
Format: Article
Language:English
Published: Springer 2025-08-01
Series:Discover Oncology
Subjects:
Colon cancer
LncRNA
CeRNA
Ferroptosis
Risk model
Gut microbes
Online Access:https://doi.org/10.1007/s12672-025-03351-z
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Summary:Abstract Background Colon cancer, a globally prevalent malignancy with high mortality, involves lncRNA regulation, ferroptosis pathway abnormalities, and gut microbiota dysbiosis. Ferroptosis-related gene models may aid prognostic evaluation, while microbiota metabolites modulate ferroptosis in contexts like ulcerative colitis. Methods Using the GEO dataset (GSE97300), we screened differentially expressed lncRNAs (e.g., AC002331.1). Competing endogenous RNA networks were predicted via miRcode and miRTarBase, followed by integration with FerrDb to establish ferroptosis regulatory relationships. GutMGene analyzed microbiota metabolite-gene interactions. A 12-gene prognostic model (e.g., HMGB1, VEGFA) was constructed using TCGA data and WEKA 3.8, validated by Kaplan-Meier analysis and ROC curves (10-fold cross-validation). Results LncRNA AC002331.1 was upregulated in colon cancer and positively associated with improved OS/DFS (log-rank test, p < 0.05). Its ceRNA network regulated 29 ferroptosis-related genes. The prognostic model showed discriminative power (ROC AUC = 0.653–0.683). Model genes were co-regulated by microbiota metabolites (e.g.,succinate). Conclusions This study establishes the first lncRNA AC002331.1-centered ceRNA-ferroptosis prognostic model for colon cancer, revealing microbiota-metabolite interactions in disease progression. It provides novel mechanistic insights for therapeutic targeting.
ISSN:2730-6011

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