Clinical and Immunological Analysis of Cutaneous Leishmaniasis before and after Different Treatments
Amastigotes from L. (L.)amazonensis (La), L. (L.)venezuelensis (Lv), L. (V.)brasiliensis (Lb), and L. (L.)chagasi (Lch) were cultured in a free cells liquid culture medium. Patients (n=87) from a cutaneous leishmaniasis (CL) hyperendemic region receiving different treatments were followed up from Ja...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2013/657016 |
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| author | José A. O'Daly Humberto M. Spinetti Joe Gleason María B. Rodríguez |
| author_facet | José A. O'Daly Humberto M. Spinetti Joe Gleason María B. Rodríguez |
| author_sort | José A. O'Daly |
| collection | DOAJ |
| description | Amastigotes from L. (L.)amazonensis (La), L. (L.)venezuelensis (Lv), L. (V.)brasiliensis (Lb), and L. (L.)chagasi (Lch) were cultured in a free cells liquid culture medium. Patients (n=87) from a cutaneous leishmaniasis (CL) hyperendemic region receiving different treatments were followed up from January 1994 to August 2000. Time for remission of lesions were spontaneous remission (SR) 7 weeks; Glucantime (Glu) chemotherapy 9 weeks; immunotherapy with La, Lv, Lb, and Lch amastigotes Tosyl-Lysil Chloromethyl-ketone (TLCK) treated and Nonidet P-40(NP-40) extracted (VT) 7 weeks. Delayed type hypersensitivity (DTH) response with leishmanine intradermic reaction (IDR) was higher in CL patients than healthy controls (P<0.05) and increased in active secondary versus primary infection (P<0.001) with diagnostic value 1.74 for active infection and 1.81 postclinical remission. Antibodies to amastigotes characterized by Enzyme Linked Immunosorbent Assay (ELISA) decreased in sera postclinical remission versus active infections (P<0.001), with a diagnostic value from 1.50 to 1.84. Immunoblottings antigenic bands frequency as well as Integral Optical Density (IOD) Area Densitometry decreased with sera from SR, after Glu or VT treatments in CL volunteers. Intracellular parasitism is due to normal antibodies recognizing parasite antigens after inoculation by vector. VT vaccine induced mainly cellular immunity, for remission of lesions and protection from CL infection. |
| format | Article |
| id | doaj-art-2013f53690c24146a4eec2500b77bb5f |
| institution | Kabale University |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-2013f53690c24146a4eec2500b77bb5f2025-02-03T06:11:36ZengWileyJournal of Parasitology Research2090-00232090-00312013-01-01201310.1155/2013/657016657016Clinical and Immunological Analysis of Cutaneous Leishmaniasis before and after Different TreatmentsJosé A. O'Daly0Humberto M. Spinetti1Joe Gleason2María B. Rodríguez3Astralis LTD, 1076 Stuyvesant Avenue, Irvington, NJ 07111, USAInstituto Venezolano de Investigaciones Científicas (IVIC), Altos de Pipe, Km 10 Carretera Panamericana, Caracas 1010A, VenezuelaAstralis LTD, 1076 Stuyvesant Avenue, Irvington, NJ 07111, USAInstituto Venezolano de Investigaciones Científicas (IVIC), Altos de Pipe, Km 10 Carretera Panamericana, Caracas 1010A, VenezuelaAmastigotes from L. (L.)amazonensis (La), L. (L.)venezuelensis (Lv), L. (V.)brasiliensis (Lb), and L. (L.)chagasi (Lch) were cultured in a free cells liquid culture medium. Patients (n=87) from a cutaneous leishmaniasis (CL) hyperendemic region receiving different treatments were followed up from January 1994 to August 2000. Time for remission of lesions were spontaneous remission (SR) 7 weeks; Glucantime (Glu) chemotherapy 9 weeks; immunotherapy with La, Lv, Lb, and Lch amastigotes Tosyl-Lysil Chloromethyl-ketone (TLCK) treated and Nonidet P-40(NP-40) extracted (VT) 7 weeks. Delayed type hypersensitivity (DTH) response with leishmanine intradermic reaction (IDR) was higher in CL patients than healthy controls (P<0.05) and increased in active secondary versus primary infection (P<0.001) with diagnostic value 1.74 for active infection and 1.81 postclinical remission. Antibodies to amastigotes characterized by Enzyme Linked Immunosorbent Assay (ELISA) decreased in sera postclinical remission versus active infections (P<0.001), with a diagnostic value from 1.50 to 1.84. Immunoblottings antigenic bands frequency as well as Integral Optical Density (IOD) Area Densitometry decreased with sera from SR, after Glu or VT treatments in CL volunteers. Intracellular parasitism is due to normal antibodies recognizing parasite antigens after inoculation by vector. VT vaccine induced mainly cellular immunity, for remission of lesions and protection from CL infection.http://dx.doi.org/10.1155/2013/657016 |
| spellingShingle | José A. O'Daly Humberto M. Spinetti Joe Gleason María B. Rodríguez Clinical and Immunological Analysis of Cutaneous Leishmaniasis before and after Different Treatments Journal of Parasitology Research |
| title | Clinical and Immunological Analysis of Cutaneous Leishmaniasis before and after Different Treatments |
| title_full | Clinical and Immunological Analysis of Cutaneous Leishmaniasis before and after Different Treatments |
| title_fullStr | Clinical and Immunological Analysis of Cutaneous Leishmaniasis before and after Different Treatments |
| title_full_unstemmed | Clinical and Immunological Analysis of Cutaneous Leishmaniasis before and after Different Treatments |
| title_short | Clinical and Immunological Analysis of Cutaneous Leishmaniasis before and after Different Treatments |
| title_sort | clinical and immunological analysis of cutaneous leishmaniasis before and after different treatments |
| url | http://dx.doi.org/10.1155/2013/657016 |
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