The long and winding road of reverse genetics in Trypanosoma cruzi
Trypanosomes are early divergent protists with distinctive features among eukaryotic cells. Together with Trypanosoma brucei and Leishmania spp., Trypanosoma cruzi has been one of the most studied members of the group. This protozoan parasite is the causative agent of Chagas disease, a leading cause...
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| Language: | English |
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Shared Science Publishers OG
2021-08-01
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| Series: | Microbial Cell |
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| Online Access: | http://microbialcell.com/researcharticles/2021a-chiurillo-microbial-cell/ |
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| author | Miguel A. Chiurillo Noelia Lander |
| author_facet | Miguel A. Chiurillo Noelia Lander |
| author_sort | Miguel A. Chiurillo |
| collection | DOAJ |
| description | Trypanosomes are early divergent protists with distinctive features among eukaryotic cells. Together with Trypanosoma brucei and Leishmania spp., Trypanosoma cruzi has been one of the most studied members of the group. This protozoan parasite is the causative agent of Chagas disease, a leading cause of heart disease in the Americas, for which there is no vaccine or satisfactory treatment available. Understanding T. cruzi biology is crucial to identify alternative targets for antiparasitic interventions. Genetic manipulation of T. cruzi has been historically challenging. However, the emergence of CRISPR/Cas9 technology has significantly improved the ability to generate genetically modified T. cruzi cell lines. Still, the system alone is not sufficient to answer all biologically relevant questions. In general, current genetic methods have limitations that should be overcome to advance in the study of this peculiar parasite. In this brief historic overview, we highlight the strengths and weaknesses of the molecular strategies that have been developed to genetically modify T. cruzi, emphasizing the future directions of the field. |
| format | Article |
| id | doaj-art-2010784b6c2d4fb29ff32ecb540a1c8a |
| institution | DOAJ |
| issn | 2311-2638 |
| language | English |
| publishDate | 2021-08-01 |
| publisher | Shared Science Publishers OG |
| record_format | Article |
| series | Microbial Cell |
| spelling | doaj-art-2010784b6c2d4fb29ff32ecb540a1c8a2025-08-20T02:53:19ZengShared Science Publishers OGMicrobial Cell2311-26382021-08-018920320710.15698/mic2021.09.758The long and winding road of reverse genetics in Trypanosoma cruziMiguel A. Chiurillo0Noelia Lander1Department of Biological Sciences, University of Cincinnati, Cincinnati, OH 45221, USA.Department of Biological Sciences, University of Cincinnati, Cincinnati, OH 45221, USA.Trypanosomes are early divergent protists with distinctive features among eukaryotic cells. Together with Trypanosoma brucei and Leishmania spp., Trypanosoma cruzi has been one of the most studied members of the group. This protozoan parasite is the causative agent of Chagas disease, a leading cause of heart disease in the Americas, for which there is no vaccine or satisfactory treatment available. Understanding T. cruzi biology is crucial to identify alternative targets for antiparasitic interventions. Genetic manipulation of T. cruzi has been historically challenging. However, the emergence of CRISPR/Cas9 technology has significantly improved the ability to generate genetically modified T. cruzi cell lines. Still, the system alone is not sufficient to answer all biologically relevant questions. In general, current genetic methods have limitations that should be overcome to advance in the study of this peculiar parasite. In this brief historic overview, we highlight the strengths and weaknesses of the molecular strategies that have been developed to genetically modify T. cruzi, emphasizing the future directions of the field.http://microbialcell.com/researcharticles/2021a-chiurillo-microbial-cell/chagas diseasecrispr/cas9gene knockoutgene tagginggene knock-ingenome editingtrypanosomes |
| spellingShingle | Miguel A. Chiurillo Noelia Lander The long and winding road of reverse genetics in Trypanosoma cruzi Microbial Cell chagas disease crispr/cas9 gene knockout gene tagging gene knock-in genome editing trypanosomes |
| title | The long and winding road of reverse genetics in Trypanosoma cruzi |
| title_full | The long and winding road of reverse genetics in Trypanosoma cruzi |
| title_fullStr | The long and winding road of reverse genetics in Trypanosoma cruzi |
| title_full_unstemmed | The long and winding road of reverse genetics in Trypanosoma cruzi |
| title_short | The long and winding road of reverse genetics in Trypanosoma cruzi |
| title_sort | long and winding road of reverse genetics in trypanosoma cruzi |
| topic | chagas disease crispr/cas9 gene knockout gene tagging gene knock-in genome editing trypanosomes |
| url | http://microbialcell.com/researcharticles/2021a-chiurillo-microbial-cell/ |
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