Diosgenin Induces Apoptosis of MCF-7 Cells by Regulating DAXX Subcellular Localization and Activating JNK/p38 Signaling Pathway
ObjectiveTo investigate the effect of diosgenin on the proliferation and apoptosis of breast cancer cells and its potential molecular mechanism. MethodsThe breast cancer cell line MCF-7 was treated with low, medium, and high doses of diosgenin, and cell proliferation was detected through the MMT met...
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Magazine House of Cancer Research on Prevention and Treatment
2025-05-01
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| Series: | Zhongliu Fangzhi Yanjiu |
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| Online Access: | http://www.zlfzyj.com/cn/article/doi/10.3971/j.issn.1000-8578.2025.24.0980 |
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| _version_ | 1850123745484603392 |
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| author | Jia WANG Shilei GAO Lihan ZHANG Lu ZHANG Xu SUN Huahua LI Huaimin LIU |
| author_facet | Jia WANG Shilei GAO Lihan ZHANG Lu ZHANG Xu SUN Huahua LI Huaimin LIU |
| author_sort | Jia WANG |
| collection | DOAJ |
| description | ObjectiveTo investigate the effect of diosgenin on the proliferation and apoptosis of breast cancer cells and its potential molecular mechanism. MethodsThe breast cancer cell line MCF-7 was treated with low, medium, and high doses of diosgenin, and cell proliferation was detected through the MMT method. Flow cytometry was used to detect cell apoptosis. Nuclear-cytoplasmic-protein separation method was applied to detect the subcellular localization of death associated protein (DAXX). qRT-PCR and Western blot were used to detect the expressions of DAXX and c-Jun N-terminal kinase pathway (JNK)-related proteins. ResultsDiosgenin considerably inhibited the proliferation of MCF-7 cells and promoted cell apoptosis in a concentration-dependent manner. Diosgenin can promote the movement of DAXX from nucleus into the cytoplasm. Diosgenin upregulated the expression of cell surface death receptor (Fas), increased the phosphorylation levels of JNK and mitogen activated protein kinase (p38), and activated the JNK/p38 signaling pathway with concentration dependence. ConclusionDiosgenin inhibits the proliferation and promotes the apoptosis of the breast cancer cell line MCF-7, whose mechanism may be related to the regulation of DAXX subcellular localization and the activation of JNK/p38 signaling pathway. |
| format | Article |
| id | doaj-art-201038e5423d469b9fa87f39d61db6e6 |
| institution | OA Journals |
| issn | 1000-8578 |
| language | zho |
| publishDate | 2025-05-01 |
| publisher | Magazine House of Cancer Research on Prevention and Treatment |
| record_format | Article |
| series | Zhongliu Fangzhi Yanjiu |
| spelling | doaj-art-201038e5423d469b9fa87f39d61db6e62025-08-20T02:34:32ZzhoMagazine House of Cancer Research on Prevention and TreatmentZhongliu Fangzhi Yanjiu1000-85782025-05-0152536837310.3971/j.issn.1000-8578.2025.24.098020240980Diosgenin Induces Apoptosis of MCF-7 Cells by Regulating DAXX Subcellular Localization and Activating JNK/p38 Signaling PathwayJia WANG0Shilei GAO1Lihan ZHANG2Lu ZHANG3Xu SUN4Huahua LI5Huaimin LIU6Department of Integrated Traditional and Western Medicine, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, Zhengzhou 450008, ChinaDepartment of Bone and Soft Tissue, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, Zhengzhou 450008, ChinaDepartment of Integrated Traditional and Western Medicine, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, Zhengzhou 450008, ChinaDepartment of Integrated Traditional and Western Medicine, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, Zhengzhou 450008, ChinaDepartment of Integrated Traditional and Western Medicine, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, Zhengzhou 450008, ChinaDepartment of Integrated Traditional and Western Medicine, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, Zhengzhou 450008, ChinaDepartment of Integrated Traditional and Western Medicine, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, Zhengzhou 450008, ChinaObjectiveTo investigate the effect of diosgenin on the proliferation and apoptosis of breast cancer cells and its potential molecular mechanism. MethodsThe breast cancer cell line MCF-7 was treated with low, medium, and high doses of diosgenin, and cell proliferation was detected through the MMT method. Flow cytometry was used to detect cell apoptosis. Nuclear-cytoplasmic-protein separation method was applied to detect the subcellular localization of death associated protein (DAXX). qRT-PCR and Western blot were used to detect the expressions of DAXX and c-Jun N-terminal kinase pathway (JNK)-related proteins. ResultsDiosgenin considerably inhibited the proliferation of MCF-7 cells and promoted cell apoptosis in a concentration-dependent manner. Diosgenin can promote the movement of DAXX from nucleus into the cytoplasm. Diosgenin upregulated the expression of cell surface death receptor (Fas), increased the phosphorylation levels of JNK and mitogen activated protein kinase (p38), and activated the JNK/p38 signaling pathway with concentration dependence. ConclusionDiosgenin inhibits the proliferation and promotes the apoptosis of the breast cancer cell line MCF-7, whose mechanism may be related to the regulation of DAXX subcellular localization and the activation of JNK/p38 signaling pathway.http://www.zlfzyj.com/cn/article/doi/10.3971/j.issn.1000-8578.2025.24.0980breast cancerdiosgenindaxxjnk/p38 signaling pathway |
| spellingShingle | Jia WANG Shilei GAO Lihan ZHANG Lu ZHANG Xu SUN Huahua LI Huaimin LIU Diosgenin Induces Apoptosis of MCF-7 Cells by Regulating DAXX Subcellular Localization and Activating JNK/p38 Signaling Pathway Zhongliu Fangzhi Yanjiu breast cancer diosgenin daxx jnk/p38 signaling pathway |
| title | Diosgenin Induces Apoptosis of MCF-7 Cells by Regulating DAXX Subcellular Localization and Activating JNK/p38 Signaling Pathway |
| title_full | Diosgenin Induces Apoptosis of MCF-7 Cells by Regulating DAXX Subcellular Localization and Activating JNK/p38 Signaling Pathway |
| title_fullStr | Diosgenin Induces Apoptosis of MCF-7 Cells by Regulating DAXX Subcellular Localization and Activating JNK/p38 Signaling Pathway |
| title_full_unstemmed | Diosgenin Induces Apoptosis of MCF-7 Cells by Regulating DAXX Subcellular Localization and Activating JNK/p38 Signaling Pathway |
| title_short | Diosgenin Induces Apoptosis of MCF-7 Cells by Regulating DAXX Subcellular Localization and Activating JNK/p38 Signaling Pathway |
| title_sort | diosgenin induces apoptosis of mcf 7 cells by regulating daxx subcellular localization and activating jnk p38 signaling pathway |
| topic | breast cancer diosgenin daxx jnk/p38 signaling pathway |
| url | http://www.zlfzyj.com/cn/article/doi/10.3971/j.issn.1000-8578.2025.24.0980 |
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