Assessment of in ovo soursop (Annona muricata L.) leaf extract injection on intestinal microbes in Noiler chicks
Soursop leaves possess valuable phytochemicals that can improve the health performance of chickens leading to production efficiency. This study was designed to assess the effect of in ovo injection of soursop leaf extract (SLE) on the intestinal microbes of Noiler chicks. A total of 640 incubated fe...
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| Language: | English |
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Elsevier
2025-03-01
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| Series: | The Microbe |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2950194625000202 |
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| author | Timothy T. Kuka Simplice D. Karou Anani Kokou Batomayena Bakoma |
| author_facet | Timothy T. Kuka Simplice D. Karou Anani Kokou Batomayena Bakoma |
| author_sort | Timothy T. Kuka |
| collection | DOAJ |
| description | Soursop leaves possess valuable phytochemicals that can improve the health performance of chickens leading to production efficiency. This study was designed to assess the effect of in ovo injection of soursop leaf extract (SLE) on the intestinal microbes of Noiler chicks. A total of 640 incubated fertile eggs were randomly distributed into four groups: 0.25 µg SLE, 0.5 µg SLE, 0.75 µg/ml SLE, and a non-injected control group on day 18 of incubation. Each of the three treatment groups received 0.2 ml of SLE injected into the airspace of the eggs. Hatched chicks from the groups were divided into four replicates and reared in a completely randomized design. At ten days of age, two chicks per replicate were sacrificed, caeca contents of the chicks were collected aseptically and used for microbial enumeration following standard microbial procedures and specific agars. Data collected was subjected to analysis of variance and means were compared using Tukey’s HSD at p ≤ 0.05. Results showed that E. coli count was significantly lower (p < 0.05) in the group with the highest concentration of SLE, this is due to the antibacterial activities of soursop leaf extract. It was concluded that in ovo injection of SLE at 0.75 µg confers intestinal health by reducing caeca E. coli count in Noiler chicks. |
| format | Article |
| id | doaj-art-200ebe42f0cb42779506ed095394270a |
| institution | Kabale University |
| issn | 2950-1946 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | The Microbe |
| spelling | doaj-art-200ebe42f0cb42779506ed095394270a2025-02-07T04:48:38ZengElsevierThe Microbe2950-19462025-03-016100252Assessment of in ovo soursop (Annona muricata L.) leaf extract injection on intestinal microbes in Noiler chicksTimothy T. Kuka0Simplice D. Karou1Anani Kokou2Batomayena Bakoma3Regional Center of Excellence in Poultry Science, University of Lome, Lome BSP 1515, Togo; Corresponding author.Laboratory of Microbiology and Quality Control of Foodstuffs (LAMICODA), Higher School of Biological and Food Techniques (ESTBA), University of Lome, TogoLaboratory of Microbiology and Quality Control of Foodstuffs (LAMICODA), Higher School of Biological and Food Techniques (ESTBA), University of Lome, TogoPharmaceutical Sciences Research Laboratory, University of Lome, Lome BSP 1515, TogoSoursop leaves possess valuable phytochemicals that can improve the health performance of chickens leading to production efficiency. This study was designed to assess the effect of in ovo injection of soursop leaf extract (SLE) on the intestinal microbes of Noiler chicks. A total of 640 incubated fertile eggs were randomly distributed into four groups: 0.25 µg SLE, 0.5 µg SLE, 0.75 µg/ml SLE, and a non-injected control group on day 18 of incubation. Each of the three treatment groups received 0.2 ml of SLE injected into the airspace of the eggs. Hatched chicks from the groups were divided into four replicates and reared in a completely randomized design. At ten days of age, two chicks per replicate were sacrificed, caeca contents of the chicks were collected aseptically and used for microbial enumeration following standard microbial procedures and specific agars. Data collected was subjected to analysis of variance and means were compared using Tukey’s HSD at p ≤ 0.05. Results showed that E. coli count was significantly lower (p < 0.05) in the group with the highest concentration of SLE, this is due to the antibacterial activities of soursop leaf extract. It was concluded that in ovo injection of SLE at 0.75 µg confers intestinal health by reducing caeca E. coli count in Noiler chicks.http://www.sciencedirect.com/science/article/pii/S2950194625000202E. coliIntestinal microbesIn ovoLeaf extractNoilerSoursop |
| spellingShingle | Timothy T. Kuka Simplice D. Karou Anani Kokou Batomayena Bakoma Assessment of in ovo soursop (Annona muricata L.) leaf extract injection on intestinal microbes in Noiler chicks The Microbe E. coli Intestinal microbes In ovo Leaf extract Noiler Soursop |
| title | Assessment of in ovo soursop (Annona muricata L.) leaf extract injection on intestinal microbes in Noiler chicks |
| title_full | Assessment of in ovo soursop (Annona muricata L.) leaf extract injection on intestinal microbes in Noiler chicks |
| title_fullStr | Assessment of in ovo soursop (Annona muricata L.) leaf extract injection on intestinal microbes in Noiler chicks |
| title_full_unstemmed | Assessment of in ovo soursop (Annona muricata L.) leaf extract injection on intestinal microbes in Noiler chicks |
| title_short | Assessment of in ovo soursop (Annona muricata L.) leaf extract injection on intestinal microbes in Noiler chicks |
| title_sort | assessment of in ovo soursop annona muricata l leaf extract injection on intestinal microbes in noiler chicks |
| topic | E. coli Intestinal microbes In ovo Leaf extract Noiler Soursop |
| url | http://www.sciencedirect.com/science/article/pii/S2950194625000202 |
| work_keys_str_mv | AT timothytkuka assessmentofinovosoursopannonamuricatalleafextractinjectiononintestinalmicrobesinnoilerchicks AT simplicedkarou assessmentofinovosoursopannonamuricatalleafextractinjectiononintestinalmicrobesinnoilerchicks AT ananikokou assessmentofinovosoursopannonamuricatalleafextractinjectiononintestinalmicrobesinnoilerchicks AT batomayenabakoma assessmentofinovosoursopannonamuricatalleafextractinjectiononintestinalmicrobesinnoilerchicks |