The Impact of Fucoidan Extracts on Heat-Stress-Induced Loss of In Vitro Fast-Twitch Muscle Function in Mice
Elevated temperatures have been shown to decrease muscle force production, with potential causes including protein unfolding, enzyme denaturation, and reactive oxygen species (ROS). This study aimed to investigate whether fucoidan, a compound derived from brown seaweed, could mitigate heat-stress-in...
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MDPI AG
2025-02-01
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| author | Samantha T. C. Kucewicz Stefan Piantella Jarrod E. Church Caroline J. Taylor Chris van der Poel |
| author_facet | Samantha T. C. Kucewicz Stefan Piantella Jarrod E. Church Caroline J. Taylor Chris van der Poel |
| author_sort | Samantha T. C. Kucewicz |
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| description | Elevated temperatures have been shown to decrease muscle force production, with potential causes including protein unfolding, enzyme denaturation, and reactive oxygen species (ROS). This study aimed to investigate whether fucoidan, a compound derived from brown seaweed, could mitigate heat-stress-induced loss of muscle function. C57BL/6 mice were orally administered fucoidan (400 mg/kg/day) from one of two different seaweed species Fucus vesiculosus (FVF) or Undaria pinnatifida (UPF) or vehicle control for seven consecutive days. Subsequently, the in vitro muscle function of the fast-twitch extensor digitorum longus (EDL) was assessed at either 25 °C (control) or 43 °C (heat stress). Functional analysis was complemented with gene analysis and the C2C12 myoblast heat-stress assay. The temperature (43 °C)-induced loss of force produced by the EDL muscle was significantly attenuated by fucoidan from FVF but not UPF. Fucoidan from UPF did not affect gene expression levels, whereas fucoidan from FVF significantly increased the expression levels of HSP90. In mouse C2C12 myoblasts, heat stress induced a significant increase in ROS production which was significantly reduced by both fucoidan species. These results suggest fucoidan extracted from Fucus vesiculosus may be an effective preventive strategy to protect against heat-induced loss of muscle strength in fast-twitch muscles. |
| format | Article |
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| institution | DOAJ |
| issn | 2813-0413 |
| language | English |
| publishDate | 2025-02-01 |
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| spelling | doaj-art-200c2c0fe6374322b2bc569e03ef7eb32025-08-20T02:42:28ZengMDPI AGMuscles2813-04132025-02-0141610.3390/muscles4010006The Impact of Fucoidan Extracts on Heat-Stress-Induced Loss of In Vitro Fast-Twitch Muscle Function in MiceSamantha T. C. Kucewicz0Stefan Piantella1Jarrod E. Church2Caroline J. Taylor3Chris van der Poel4Department of Microbiology, Anatomy, Physiology & Pharmacology, School of Life Sciences, La Trobe University, Bundoora, VIC 3086, AustraliaDepartment of Psychology, Counselling & Therapy, School of Psychology and Public Health, La Trobe University, Bundoora, VIC 3086, AustraliaDepartment of Microbiology, Anatomy, Physiology & Pharmacology, School of Life Sciences, La Trobe University, Bundoora, VIC 3086, AustraliaDepartment of Microbiology, Anatomy, Physiology & Pharmacology, School of Life Sciences, La Trobe University, Bundoora, VIC 3086, AustraliaDepartment of Microbiology, Anatomy, Physiology & Pharmacology, School of Life Sciences, La Trobe University, Bundoora, VIC 3086, AustraliaElevated temperatures have been shown to decrease muscle force production, with potential causes including protein unfolding, enzyme denaturation, and reactive oxygen species (ROS). This study aimed to investigate whether fucoidan, a compound derived from brown seaweed, could mitigate heat-stress-induced loss of muscle function. C57BL/6 mice were orally administered fucoidan (400 mg/kg/day) from one of two different seaweed species Fucus vesiculosus (FVF) or Undaria pinnatifida (UPF) or vehicle control for seven consecutive days. Subsequently, the in vitro muscle function of the fast-twitch extensor digitorum longus (EDL) was assessed at either 25 °C (control) or 43 °C (heat stress). Functional analysis was complemented with gene analysis and the C2C12 myoblast heat-stress assay. The temperature (43 °C)-induced loss of force produced by the EDL muscle was significantly attenuated by fucoidan from FVF but not UPF. Fucoidan from UPF did not affect gene expression levels, whereas fucoidan from FVF significantly increased the expression levels of HSP90. In mouse C2C12 myoblasts, heat stress induced a significant increase in ROS production which was significantly reduced by both fucoidan species. These results suggest fucoidan extracted from Fucus vesiculosus may be an effective preventive strategy to protect against heat-induced loss of muscle strength in fast-twitch muscles.https://www.mdpi.com/2813-0413/4/1/6skeletal muscle contractionheat stressfucoidanFucus vesiculosusUndaria pinnatifida |
| spellingShingle | Samantha T. C. Kucewicz Stefan Piantella Jarrod E. Church Caroline J. Taylor Chris van der Poel The Impact of Fucoidan Extracts on Heat-Stress-Induced Loss of In Vitro Fast-Twitch Muscle Function in Mice Muscles skeletal muscle contraction heat stress fucoidan Fucus vesiculosus Undaria pinnatifida |
| title | The Impact of Fucoidan Extracts on Heat-Stress-Induced Loss of In Vitro Fast-Twitch Muscle Function in Mice |
| title_full | The Impact of Fucoidan Extracts on Heat-Stress-Induced Loss of In Vitro Fast-Twitch Muscle Function in Mice |
| title_fullStr | The Impact of Fucoidan Extracts on Heat-Stress-Induced Loss of In Vitro Fast-Twitch Muscle Function in Mice |
| title_full_unstemmed | The Impact of Fucoidan Extracts on Heat-Stress-Induced Loss of In Vitro Fast-Twitch Muscle Function in Mice |
| title_short | The Impact of Fucoidan Extracts on Heat-Stress-Induced Loss of In Vitro Fast-Twitch Muscle Function in Mice |
| title_sort | impact of fucoidan extracts on heat stress induced loss of in vitro fast twitch muscle function in mice |
| topic | skeletal muscle contraction heat stress fucoidan Fucus vesiculosus Undaria pinnatifida |
| url | https://www.mdpi.com/2813-0413/4/1/6 |
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