Antitumoral properties of the pomegranate peel and blueberry extracts against tongue carcinoma (in vitro study)
Background: Oral squamous cell carcinoma (OSCC) is one of the most common cancers globally. Considering the drawbacks of the traditional treatment phytochemicals have been introduced to the research field with consideration of their minimal, or no side effects and good efficacy against cancer cells....
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| Language: | English |
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Springer
2023-12-01
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| Series: | Saudi Dental Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1013905223001505 |
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| author | Reham Mostafa Ahmed Doaa A.M. Esmaeil Shrief Youssef El-Nagdy Nadia Abbas El-Sisi |
| author_facet | Reham Mostafa Ahmed Doaa A.M. Esmaeil Shrief Youssef El-Nagdy Nadia Abbas El-Sisi |
| author_sort | Reham Mostafa Ahmed |
| collection | DOAJ |
| description | Background: Oral squamous cell carcinoma (OSCC) is one of the most common cancers globally. Considering the drawbacks of the traditional treatment phytochemicals have been introduced to the research field with consideration of their minimal, or no side effects and good efficacy against cancer cells. Pomegranate peel and blueberries are very well-known phytochemicals in this context. Material and methods: Methanolic extracts are prepared from both pomegranate peel (PPE) and blueberry (BE) dried powders. The antioxidant content was determined by ORAC assay for both extracts. The cytotoxic levels of the extracts and IC50 on the HNO-97 cell line were defined using the colorimetric SRB assay. Then flow cytometric apoptosis analysis of the IC50 of both extracts was performed. Finally, an investigation of the metastasis through the wound healing assay was performed for both extracts. Results: A significant difference in the antioxidant content was found between PPE and BE ethanolic extracts. The Ic50 for the PPE-treated cell line was 73.35 µg/ml while the BE showed it at 525.38 µg/ml with a significant difference between them. Both PPE and BE showed significant induction of cancer cell apoptosis with much better results with PPE treatment. The wound healing assay showed significant inhibition of cell migration when treated with PPE while there was not any significant effect on cell migration when treated with BE. Conclusion: With the consideration that, the Phytochemicals used are well-known fruits with no harm on normal tissues. Low doses of PPE exert incredibly significant alteration in the HNO-97 tongue cancer cell proliferation, inducing apoptosis and inhibition of cancer cell migration. Meanwhile, treatment with BE needs much higher doses for showing anti-proliferative properties of cancer cells and induce cancer cell apoptosis. This gives promising results for further investigations about using them as a treatment or adjunctive treatment for oral cancer cases. |
| format | Article |
| id | doaj-art-200be7f0e035442db6602a3e561c55d8 |
| institution | DOAJ |
| issn | 1013-9052 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Springer |
| record_format | Article |
| series | Saudi Dental Journal |
| spelling | doaj-art-200be7f0e035442db6602a3e561c55d82025-08-20T03:17:44ZengSpringerSaudi Dental Journal1013-90522023-12-0135898599510.1016/j.sdentj.2023.07.021Antitumoral properties of the pomegranate peel and blueberry extracts against tongue carcinoma (in vitro study)Reham Mostafa Ahmed0Doaa A.M. Esmaeil1Shrief Youssef El-Nagdy2Nadia Abbas El-Sisi3Oral Pathology Department, Faculty of Dentistry, Mansoura University, Egypt; Corresponding author.Oral Pathology Department, Faculty of Dentistry, Mansoura University, Egypt; Oral Pathology Department, Sini University, Kantra Campus, EgyptOral Pathology Department, Faculty of Dentistry, Mansoura University, Egypt; Oral Pathology Department, Faculty of Dentistry, Horus University, EgyptOral Pathology Department, Faculty of Dentistry, Mansoura University, EgyptBackground: Oral squamous cell carcinoma (OSCC) is one of the most common cancers globally. Considering the drawbacks of the traditional treatment phytochemicals have been introduced to the research field with consideration of their minimal, or no side effects and good efficacy against cancer cells. Pomegranate peel and blueberries are very well-known phytochemicals in this context. Material and methods: Methanolic extracts are prepared from both pomegranate peel (PPE) and blueberry (BE) dried powders. The antioxidant content was determined by ORAC assay for both extracts. The cytotoxic levels of the extracts and IC50 on the HNO-97 cell line were defined using the colorimetric SRB assay. Then flow cytometric apoptosis analysis of the IC50 of both extracts was performed. Finally, an investigation of the metastasis through the wound healing assay was performed for both extracts. Results: A significant difference in the antioxidant content was found between PPE and BE ethanolic extracts. The Ic50 for the PPE-treated cell line was 73.35 µg/ml while the BE showed it at 525.38 µg/ml with a significant difference between them. Both PPE and BE showed significant induction of cancer cell apoptosis with much better results with PPE treatment. The wound healing assay showed significant inhibition of cell migration when treated with PPE while there was not any significant effect on cell migration when treated with BE. Conclusion: With the consideration that, the Phytochemicals used are well-known fruits with no harm on normal tissues. Low doses of PPE exert incredibly significant alteration in the HNO-97 tongue cancer cell proliferation, inducing apoptosis and inhibition of cancer cell migration. Meanwhile, treatment with BE needs much higher doses for showing anti-proliferative properties of cancer cells and induce cancer cell apoptosis. This gives promising results for further investigations about using them as a treatment or adjunctive treatment for oral cancer cases.http://www.sciencedirect.com/science/article/pii/S1013905223001505PhytochemicalsOral cancerPomegranatePomegranate peelBlueberryCancer treatment |
| spellingShingle | Reham Mostafa Ahmed Doaa A.M. Esmaeil Shrief Youssef El-Nagdy Nadia Abbas El-Sisi Antitumoral properties of the pomegranate peel and blueberry extracts against tongue carcinoma (in vitro study) Saudi Dental Journal Phytochemicals Oral cancer Pomegranate Pomegranate peel Blueberry Cancer treatment |
| title | Antitumoral properties of the pomegranate peel and blueberry extracts against tongue carcinoma (in vitro study) |
| title_full | Antitumoral properties of the pomegranate peel and blueberry extracts against tongue carcinoma (in vitro study) |
| title_fullStr | Antitumoral properties of the pomegranate peel and blueberry extracts against tongue carcinoma (in vitro study) |
| title_full_unstemmed | Antitumoral properties of the pomegranate peel and blueberry extracts against tongue carcinoma (in vitro study) |
| title_short | Antitumoral properties of the pomegranate peel and blueberry extracts against tongue carcinoma (in vitro study) |
| title_sort | antitumoral properties of the pomegranate peel and blueberry extracts against tongue carcinoma in vitro study |
| topic | Phytochemicals Oral cancer Pomegranate Pomegranate peel Blueberry Cancer treatment |
| url | http://www.sciencedirect.com/science/article/pii/S1013905223001505 |
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