Blue Light Enhances the Antioxidant, Antimicrobial, and Antitumor Potential of the Green Microalgae <i>Coelastrella</i> sp. BGV

Green algae of the genus <i>Coelastrella</i> have attracted the attention of scientists due to their rich biochemical composition and potential for application in phytomedicine. The present study investigated the influence of light on the bioactive capacity of extracts from the Bulgarian...

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Main Authors: Zhaneta Georgieva, Zornitsa Karcheva, Tanya Toshkova-Yotova, Ani Georgieva, Reneta Toshkova, Detelina Petrova, Miroslava Zhiponova, Ganka Chaneva
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Plants
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Online Access:https://www.mdpi.com/2223-7747/13/23/3295
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author Zhaneta Georgieva
Zornitsa Karcheva
Tanya Toshkova-Yotova
Ani Georgieva
Reneta Toshkova
Detelina Petrova
Miroslava Zhiponova
Ganka Chaneva
author_facet Zhaneta Georgieva
Zornitsa Karcheva
Tanya Toshkova-Yotova
Ani Georgieva
Reneta Toshkova
Detelina Petrova
Miroslava Zhiponova
Ganka Chaneva
author_sort Zhaneta Georgieva
collection DOAJ
description Green algae of the genus <i>Coelastrella</i> have attracted the attention of scientists due to their rich biochemical composition and potential for application in phytomedicine. The present study investigated the influence of light on the bioactive capacity of extracts from the Bulgarian strain of the green microalgae <i>Coelastrella</i> sp. BGV. Three LED lights were examined—red/blue (C1), blue (C2), and control white light (C3). The respective ethanol extracts were analyzed for the total content of phenolic antioxidants. The antimicrobial activity was tested using the disk-diffusion method against 10 microorganisms. The antiproliferative and cytotoxic effects on cervical carcinoma HeLa and hepatocellular carcinoma HepG2 cell lines, as well as non-tumorigenic embryonal fibroblasts BALB/3T3 control, were evaluated using a cell viability assay. The overall results highlighted blue light as a factor enhancing the antioxidant, antibacterial, and cytotoxic activities of the C2 microalgal extract. Additionally, the investigated mechanism of the antitumor activity revealed a proapoptotic effect. In contrast, the C1 extract exhibited weaker activity and selectivity, while the C3 extract was the least active but demonstrated high cytotoxic selectivity. This study could contribute to expanding knowledge about the high biological potential of green microalgae and the development of biotechnological approaches for its regulation.
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spelling doaj-art-2001f3e52b014b5180c234e90f90a35b2025-08-20T02:50:40ZengMDPI AGPlants2223-77472024-11-011323329510.3390/plants13233295Blue Light Enhances the Antioxidant, Antimicrobial, and Antitumor Potential of the Green Microalgae <i>Coelastrella</i> sp. BGVZhaneta Georgieva0Zornitsa Karcheva1Tanya Toshkova-Yotova2Ani Georgieva3Reneta Toshkova4Detelina Petrova5Miroslava Zhiponova6Ganka Chaneva7Department of Plant Physiology, Faculty of Biology, Sofia University “St. Kliment Ohridski”, 1164 Sofia, BulgariaDepartment of Plant Physiology, Faculty of Biology, Sofia University “St. Kliment Ohridski”, 1164 Sofia, BulgariaLaboratory “Experimental and Applied Algology”, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaInstitute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaInstitute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaDepartment of Plant Physiology, Faculty of Biology, Sofia University “St. Kliment Ohridski”, 1164 Sofia, BulgariaDepartment of Plant Physiology, Faculty of Biology, Sofia University “St. Kliment Ohridski”, 1164 Sofia, BulgariaDepartment of Plant Physiology, Faculty of Biology, Sofia University “St. Kliment Ohridski”, 1164 Sofia, BulgariaGreen algae of the genus <i>Coelastrella</i> have attracted the attention of scientists due to their rich biochemical composition and potential for application in phytomedicine. The present study investigated the influence of light on the bioactive capacity of extracts from the Bulgarian strain of the green microalgae <i>Coelastrella</i> sp. BGV. Three LED lights were examined—red/blue (C1), blue (C2), and control white light (C3). The respective ethanol extracts were analyzed for the total content of phenolic antioxidants. The antimicrobial activity was tested using the disk-diffusion method against 10 microorganisms. The antiproliferative and cytotoxic effects on cervical carcinoma HeLa and hepatocellular carcinoma HepG2 cell lines, as well as non-tumorigenic embryonal fibroblasts BALB/3T3 control, were evaluated using a cell viability assay. The overall results highlighted blue light as a factor enhancing the antioxidant, antibacterial, and cytotoxic activities of the C2 microalgal extract. Additionally, the investigated mechanism of the antitumor activity revealed a proapoptotic effect. In contrast, the C1 extract exhibited weaker activity and selectivity, while the C3 extract was the least active but demonstrated high cytotoxic selectivity. This study could contribute to expanding knowledge about the high biological potential of green microalgae and the development of biotechnological approaches for its regulation.https://www.mdpi.com/2223-7747/13/23/3295antimicrobial activityantioxidant activityantitumor activity<i>Coelastrella</i>green microalgaeLED
spellingShingle Zhaneta Georgieva
Zornitsa Karcheva
Tanya Toshkova-Yotova
Ani Georgieva
Reneta Toshkova
Detelina Petrova
Miroslava Zhiponova
Ganka Chaneva
Blue Light Enhances the Antioxidant, Antimicrobial, and Antitumor Potential of the Green Microalgae <i>Coelastrella</i> sp. BGV
Plants
antimicrobial activity
antioxidant activity
antitumor activity
<i>Coelastrella</i>
green microalgae
LED
title Blue Light Enhances the Antioxidant, Antimicrobial, and Antitumor Potential of the Green Microalgae <i>Coelastrella</i> sp. BGV
title_full Blue Light Enhances the Antioxidant, Antimicrobial, and Antitumor Potential of the Green Microalgae <i>Coelastrella</i> sp. BGV
title_fullStr Blue Light Enhances the Antioxidant, Antimicrobial, and Antitumor Potential of the Green Microalgae <i>Coelastrella</i> sp. BGV
title_full_unstemmed Blue Light Enhances the Antioxidant, Antimicrobial, and Antitumor Potential of the Green Microalgae <i>Coelastrella</i> sp. BGV
title_short Blue Light Enhances the Antioxidant, Antimicrobial, and Antitumor Potential of the Green Microalgae <i>Coelastrella</i> sp. BGV
title_sort blue light enhances the antioxidant antimicrobial and antitumor potential of the green microalgae i coelastrella i sp bgv
topic antimicrobial activity
antioxidant activity
antitumor activity
<i>Coelastrella</i>
green microalgae
LED
url https://www.mdpi.com/2223-7747/13/23/3295
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