Osteoinductive activity of photobiomodulation in an organotypic bone model
Photobiomodulation (PBM) is a technique that harnesses non-ionizing light at specific wavelengths, triggering the modulation of metabolic pathways, engendering favourable biological outcomes that reduce inflammation and foster enhanced tissue healing and regeneration. PBM holds significant promise f...
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Elsevier
2024-02-01
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| Series: | Photodiagnosis and Photodynamic Therapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1572100023006658 |
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| author | Guilherme Silva Furtado Victor Martin Rita Araújo Pedro Sousa Gomes Andréa Dias Neves Lago |
| author_facet | Guilherme Silva Furtado Victor Martin Rita Araújo Pedro Sousa Gomes Andréa Dias Neves Lago |
| author_sort | Guilherme Silva Furtado |
| collection | DOAJ |
| description | Photobiomodulation (PBM) is a technique that harnesses non-ionizing light at specific wavelengths, triggering the modulation of metabolic pathways, engendering favourable biological outcomes that reduce inflammation and foster enhanced tissue healing and regeneration. PBM holds significant promise for bone tissue applications due to its non-invasive nature and ability to stimulate cellular activity and vascularization within the healing framework. Notwithstanding, the impact of PBM on bone functionality remains largely undisclosed, particularly in the absence of influencing factors such as pathologies or regenerative therapies. This study aims to investigate the potential effects of PBM using red (660 nm) (RED) and near-infrared (808 nm) (NIR) wavelengths within an ex vivo bone culture system - the organotypic embryonic chicken femur model. A continuous irradiation mode was used, administering a total energy dose of 1.0 J, at an intensity of 100 mW for 10 s, which was repeated four times over the course of the 11-day culture period. The primary focus is on characterizing the expression of pivotal osteoblastic genes, the maturation and deposition of collagen, and the formation of bone mineral. Exposing femora to both RED and NIR wavelengths led to a notable increase in the expression of osteochondrogenic transcription factors (i.e., SOX9 and RUNX2), correlating with enhanced mineralization. Notably, NIR irradiation further elevated the expression of bone matrix-related genes and fostered enhanced deposition and maturation of fibrillar collagen. This study demonstrates that PBM has the potential to enhance osteogenic functionality within a translational organotypic bone culture system, with the NIR wavelength showing remarkable capabilities in augmenting the formation and maturation of the collagenous matrix. |
| format | Article |
| id | doaj-art-1fff48a608824ed6a04602cb23dcddca |
| institution | DOAJ |
| issn | 1572-1000 |
| language | English |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Photodiagnosis and Photodynamic Therapy |
| spelling | doaj-art-1fff48a608824ed6a04602cb23dcddca2025-08-20T02:50:05ZengElsevierPhotodiagnosis and Photodynamic Therapy1572-10002024-02-014510393610.1016/j.pdpdt.2023.103936Osteoinductive activity of photobiomodulation in an organotypic bone modelGuilherme Silva Furtado0Victor Martin1Rita Araújo2Pedro Sousa Gomes3Andréa Dias Neves Lago4Graduate Student in the Postgraduate Program in Dentistry at the Federal University of Maranhão, Av. dos Portugueses, 1966, Bacanga, São Luís 65080-805, BrazilDDS, MSc and Graduate student at Laboratory for Bone Metabolism and Regeneration, Faculty of Dental Medicine, University of Porto, Rua Dr. Manuel Pereira da Silva, Porto 4200-393, Portugal; REQUIMTE/LAQV, University of Porto, Praça Coronel Pacheco, 15, Porto 4050-453, PortugalDDS, MSc and Graduate student at Laboratory for Bone Metabolism and Regeneration, Faculty of Dental Medicine, University of Porto, Rua Dr. Manuel Pereira da Silva, Porto 4200-393, Portugal; REQUIMTE/LAQV, University of Porto, Praça Coronel Pacheco, 15, Porto 4050-453, PortugalREQUIMTE/LAQV, University of Porto, Praça Coronel Pacheco, 15, Porto 4050-453, Portugal; DDS, MSc, PhD Full Professor at Laboratory for Bone Metabolism and Regeneration, Faculty of Dental Medicine, University of Porto, Porto, Portugal; Corresponding author at: Full Professor at Laboratory for Bone Metabolism and Regeneration, Faculty of Dental Medicine, University of Porto, Porto, Portugal, R. Dr. Manuel Pereira da Silva, 4200-393 Porto, Portugal.DDS, MSc, PhD, Associate Professor of the Postgraduate Program in Dentistry at the Federal University of Maranhão, São Luís, Maranhão, Av. dos Portugueses, 1966, Bacanga, São Luís 65080-805, BrazilPhotobiomodulation (PBM) is a technique that harnesses non-ionizing light at specific wavelengths, triggering the modulation of metabolic pathways, engendering favourable biological outcomes that reduce inflammation and foster enhanced tissue healing and regeneration. PBM holds significant promise for bone tissue applications due to its non-invasive nature and ability to stimulate cellular activity and vascularization within the healing framework. Notwithstanding, the impact of PBM on bone functionality remains largely undisclosed, particularly in the absence of influencing factors such as pathologies or regenerative therapies. This study aims to investigate the potential effects of PBM using red (660 nm) (RED) and near-infrared (808 nm) (NIR) wavelengths within an ex vivo bone culture system - the organotypic embryonic chicken femur model. A continuous irradiation mode was used, administering a total energy dose of 1.0 J, at an intensity of 100 mW for 10 s, which was repeated four times over the course of the 11-day culture period. The primary focus is on characterizing the expression of pivotal osteoblastic genes, the maturation and deposition of collagen, and the formation of bone mineral. Exposing femora to both RED and NIR wavelengths led to a notable increase in the expression of osteochondrogenic transcription factors (i.e., SOX9 and RUNX2), correlating with enhanced mineralization. Notably, NIR irradiation further elevated the expression of bone matrix-related genes and fostered enhanced deposition and maturation of fibrillar collagen. This study demonstrates that PBM has the potential to enhance osteogenic functionality within a translational organotypic bone culture system, with the NIR wavelength showing remarkable capabilities in augmenting the formation and maturation of the collagenous matrix.http://www.sciencedirect.com/science/article/pii/S1572100023006658Bone tissueEx vivo modelNear-infrared laserPhotobiomodulationRed laserLow Level Light |
| spellingShingle | Guilherme Silva Furtado Victor Martin Rita Araújo Pedro Sousa Gomes Andréa Dias Neves Lago Osteoinductive activity of photobiomodulation in an organotypic bone model Photodiagnosis and Photodynamic Therapy Bone tissue Ex vivo model Near-infrared laser Photobiomodulation Red laser Low Level Light |
| title | Osteoinductive activity of photobiomodulation in an organotypic bone model |
| title_full | Osteoinductive activity of photobiomodulation in an organotypic bone model |
| title_fullStr | Osteoinductive activity of photobiomodulation in an organotypic bone model |
| title_full_unstemmed | Osteoinductive activity of photobiomodulation in an organotypic bone model |
| title_short | Osteoinductive activity of photobiomodulation in an organotypic bone model |
| title_sort | osteoinductive activity of photobiomodulation in an organotypic bone model |
| topic | Bone tissue Ex vivo model Near-infrared laser Photobiomodulation Red laser Low Level Light |
| url | http://www.sciencedirect.com/science/article/pii/S1572100023006658 |
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