REXO2 is an oligoribonuclease active in human mitochondria.
The Escherichia coli oligoribonuclease, ORN, has a 3' to 5' exonuclease activity specific for small oligomers that is essential for cell viability. The human homologue, REXO2, has hitherto been incompletely characterized, with only its in vitro ability to degrade small single-stranded RNA...
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0064670&type=printable |
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| author | Francesco Bruni Pasqua Gramegna Jorge M A Oliveira Robert N Lightowlers Zofia M A Chrzanowska-Lightowlers |
| author_facet | Francesco Bruni Pasqua Gramegna Jorge M A Oliveira Robert N Lightowlers Zofia M A Chrzanowska-Lightowlers |
| author_sort | Francesco Bruni |
| collection | DOAJ |
| description | The Escherichia coli oligoribonuclease, ORN, has a 3' to 5' exonuclease activity specific for small oligomers that is essential for cell viability. The human homologue, REXO2, has hitherto been incompletely characterized, with only its in vitro ability to degrade small single-stranded RNA and DNA fragments documented. Here we show that the human enzyme has clear dual cellular localization being present both in cytosolic and mitochondrial fractions. Interestingly, the mitochondrial form localizes to both the intermembrane space and the matrix. Depletion of REXO2 by RNA interference causes a strong morphological phenotype in human cells, which show a disorganized network of punctate and granular mitochondria. Lack of REXO2 protein also causes a substantial decrease of mitochondrial nucleic acid content and impaired de novo mitochondrial protein synthesis. Our data constitute the first in vivo evidence for an oligoribonuclease activity in human mitochondria. |
| format | Article |
| id | doaj-art-1ffc337d212c4db39ffc5b5b1a3dd7ca |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-1ffc337d212c4db39ffc5b5b1a3dd7ca2025-08-20T02:30:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0185e6467010.1371/journal.pone.0064670REXO2 is an oligoribonuclease active in human mitochondria.Francesco BruniPasqua GramegnaJorge M A OliveiraRobert N LightowlersZofia M A Chrzanowska-LightowlersThe Escherichia coli oligoribonuclease, ORN, has a 3' to 5' exonuclease activity specific for small oligomers that is essential for cell viability. The human homologue, REXO2, has hitherto been incompletely characterized, with only its in vitro ability to degrade small single-stranded RNA and DNA fragments documented. Here we show that the human enzyme has clear dual cellular localization being present both in cytosolic and mitochondrial fractions. Interestingly, the mitochondrial form localizes to both the intermembrane space and the matrix. Depletion of REXO2 by RNA interference causes a strong morphological phenotype in human cells, which show a disorganized network of punctate and granular mitochondria. Lack of REXO2 protein also causes a substantial decrease of mitochondrial nucleic acid content and impaired de novo mitochondrial protein synthesis. Our data constitute the first in vivo evidence for an oligoribonuclease activity in human mitochondria.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0064670&type=printable |
| spellingShingle | Francesco Bruni Pasqua Gramegna Jorge M A Oliveira Robert N Lightowlers Zofia M A Chrzanowska-Lightowlers REXO2 is an oligoribonuclease active in human mitochondria. PLoS ONE |
| title | REXO2 is an oligoribonuclease active in human mitochondria. |
| title_full | REXO2 is an oligoribonuclease active in human mitochondria. |
| title_fullStr | REXO2 is an oligoribonuclease active in human mitochondria. |
| title_full_unstemmed | REXO2 is an oligoribonuclease active in human mitochondria. |
| title_short | REXO2 is an oligoribonuclease active in human mitochondria. |
| title_sort | rexo2 is an oligoribonuclease active in human mitochondria |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0064670&type=printable |
| work_keys_str_mv | AT francescobruni rexo2isanoligoribonucleaseactiveinhumanmitochondria AT pasquagramegna rexo2isanoligoribonucleaseactiveinhumanmitochondria AT jorgemaoliveira rexo2isanoligoribonucleaseactiveinhumanmitochondria AT robertnlightowlers rexo2isanoligoribonucleaseactiveinhumanmitochondria AT zofiamachrzanowskalightowlers rexo2isanoligoribonucleaseactiveinhumanmitochondria |