Evaluating the accuracy of HIV-1 viral load testing using near point of care Xpert HIV-1 system at Solwezi general hospital, Zambia
Abstract Objective HIV-1 Viral Load Testing plays a crucial role in the management of HIV/AIDS patients by quantifying the presence of HIV-1 RNA in the blood, which directly relates to viral replication and disease progression. Monitoring viral load enables healthcare professionals to evaluate the e...
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2025-07-01
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| Online Access: | https://doi.org/10.1186/s13104-025-07387-w |
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| author | Keembe Siakantu David Chisompola Aaron Tembo Konzani |
| author_facet | Keembe Siakantu David Chisompola Aaron Tembo Konzani |
| author_sort | Keembe Siakantu |
| collection | DOAJ |
| description | Abstract Objective HIV-1 Viral Load Testing plays a crucial role in the management of HIV/AIDS patients by quantifying the presence of HIV-1 RNA in the blood, which directly relates to viral replication and disease progression. Monitoring viral load enables healthcare professionals to evaluate the effectiveness of Anti-Retroviral Therapy, adapt treatment strategies, and assess the overall health status of individuals with HIV. This was a cross-sectional study to evaluate analytical performance (accuracy, precision and linearity) of the near point of care Xpert HIV-1 viral load. Results The analytical performance of the GeneXpert assay to the conventional Hologic Panther system was strong. Specifically, the GeneXpert demonstrated a sensitivity, specificity, and accuracy of 87.6%, 100%, and 98.5%, respectively, when compared to the Hologic Panther. A strong correlation between the two assays was evident (r = 0.97, p < 0.0001). Additionally, precision, as indicated by the coefficient of variation (CV), for high viral load was 1.38% for GeneXpert while for low viral load, it was 2.59%. In the linearity analysis, all data points remained within acceptable ranges, yielding correlation coefficients ≥ 0.99 for both assays. Conclusion The Xpert HIV-1 viral load assay demonstrates high efficacy in accurately identifying and quantifying HIV-1 viral loads, making it a valuable tool for HIV diagnosis and monitoring. This reliability makes it particularly suitable for critical populations requiring timely and accurate viral load monitoring. |
| format | Article |
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| institution | DOAJ |
| issn | 1756-0500 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
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| spelling | doaj-art-1ff7db14d53843929593d9e6e9942bc82025-08-20T03:04:12ZengBMCBMC Research Notes1756-05002025-07-011811610.1186/s13104-025-07387-wEvaluating the accuracy of HIV-1 viral load testing using near point of care Xpert HIV-1 system at Solwezi general hospital, ZambiaKeembe Siakantu0David Chisompola1Aaron Tembo Konzani2Pathology Laboratory Dept, Solwezi General HospitalPathology Laboratory Dept, Arthur Davison Children’s HospitalPathology Laboratory Dept, Tropical Gastroenterology and Nutrition groupAbstract Objective HIV-1 Viral Load Testing plays a crucial role in the management of HIV/AIDS patients by quantifying the presence of HIV-1 RNA in the blood, which directly relates to viral replication and disease progression. Monitoring viral load enables healthcare professionals to evaluate the effectiveness of Anti-Retroviral Therapy, adapt treatment strategies, and assess the overall health status of individuals with HIV. This was a cross-sectional study to evaluate analytical performance (accuracy, precision and linearity) of the near point of care Xpert HIV-1 viral load. Results The analytical performance of the GeneXpert assay to the conventional Hologic Panther system was strong. Specifically, the GeneXpert demonstrated a sensitivity, specificity, and accuracy of 87.6%, 100%, and 98.5%, respectively, when compared to the Hologic Panther. A strong correlation between the two assays was evident (r = 0.97, p < 0.0001). Additionally, precision, as indicated by the coefficient of variation (CV), for high viral load was 1.38% for GeneXpert while for low viral load, it was 2.59%. In the linearity analysis, all data points remained within acceptable ranges, yielding correlation coefficients ≥ 0.99 for both assays. Conclusion The Xpert HIV-1 viral load assay demonstrates high efficacy in accurately identifying and quantifying HIV-1 viral loads, making it a valuable tool for HIV diagnosis and monitoring. This reliability makes it particularly suitable for critical populations requiring timely and accurate viral load monitoring.https://doi.org/10.1186/s13104-025-07387-wAccuracyDiagnosisGeneXpertHIV-1Hologic pantherSolwezi |
| spellingShingle | Keembe Siakantu David Chisompola Aaron Tembo Konzani Evaluating the accuracy of HIV-1 viral load testing using near point of care Xpert HIV-1 system at Solwezi general hospital, Zambia BMC Research Notes Accuracy Diagnosis GeneXpert HIV-1 Hologic panther Solwezi |
| title | Evaluating the accuracy of HIV-1 viral load testing using near point of care Xpert HIV-1 system at Solwezi general hospital, Zambia |
| title_full | Evaluating the accuracy of HIV-1 viral load testing using near point of care Xpert HIV-1 system at Solwezi general hospital, Zambia |
| title_fullStr | Evaluating the accuracy of HIV-1 viral load testing using near point of care Xpert HIV-1 system at Solwezi general hospital, Zambia |
| title_full_unstemmed | Evaluating the accuracy of HIV-1 viral load testing using near point of care Xpert HIV-1 system at Solwezi general hospital, Zambia |
| title_short | Evaluating the accuracy of HIV-1 viral load testing using near point of care Xpert HIV-1 system at Solwezi general hospital, Zambia |
| title_sort | evaluating the accuracy of hiv 1 viral load testing using near point of care xpert hiv 1 system at solwezi general hospital zambia |
| topic | Accuracy Diagnosis GeneXpert HIV-1 Hologic panther Solwezi |
| url | https://doi.org/10.1186/s13104-025-07387-w |
| work_keys_str_mv | AT keembesiakantu evaluatingtheaccuracyofhiv1viralloadtestingusingnearpointofcarexperthiv1systematsolwezigeneralhospitalzambia AT davidchisompola evaluatingtheaccuracyofhiv1viralloadtestingusingnearpointofcarexperthiv1systematsolwezigeneralhospitalzambia AT aarontembokonzani evaluatingtheaccuracyofhiv1viralloadtestingusingnearpointofcarexperthiv1systematsolwezigeneralhospitalzambia |