Analysis of Modified Plant Metabolites Using Widely Targeted Metabolite Modificomics
Metabolite modifications play a critical role in enhancing plants’ adaptability to environmental changes and serve as a major source of functional diversity in metabolites. However, current metabolomics approaches are limited to targeted analyses of a small number of known modified metabolites and l...
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| Format: | Article |
| Language: | English |
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Bio-protocol LLC
2025-04-01
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| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=5259&type=0 |
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| author | Jianing Zhang Shixuan Li Yige Han Shouchuang Wang Penghui Liu Jun Yang |
| author_facet | Jianing Zhang Shixuan Li Yige Han Shouchuang Wang Penghui Liu Jun Yang |
| author_sort | Jianing Zhang |
| collection | DOAJ |
| description | Metabolite modifications play a critical role in enhancing plants’ adaptability to environmental changes and serve as a major source of functional diversity in metabolites. However, current metabolomics approaches are limited to targeted analyses of a small number of known modified metabolites and lack comprehensive, large-scale studies of plant metabolite modifications. Here, we describe a widely targeted metabolite modificomics (WTMM) strategy, developed using ultra-high-performance liquid chromatography–quadrupole linear ion trap (UHPLC-Q-Trap) and ultra-high-performance liquid chromatography–Q-Exactive Orbitrap (UHPLC-QE-Orbitrap) technologies. This strategy enables high-throughput identification and sensitive quantification of modified metabolites. Using tomato as a model, we conducted a metabolite modificomics study and constructed a WTMM database, identifying 165 novel modified metabolites. The WTMM strategy is broadly applicable and can be extended to the study of other plant species. |
| format | Article |
| id | doaj-art-1ff58416dd444b60affb5e2568fe5e61 |
| institution | OA Journals |
| issn | 2331-8325 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Bio-protocol LLC |
| record_format | Article |
| series | Bio-Protocol |
| spelling | doaj-art-1ff58416dd444b60affb5e2568fe5e612025-08-20T02:17:27ZengBio-protocol LLCBio-Protocol2331-83252025-04-0115710.21769/BioProtoc.5259Analysis of Modified Plant Metabolites Using Widely Targeted Metabolite ModificomicsJianing Zhang0Shixuan Li1Yige Han2Shouchuang Wang3Penghui Liu4Jun Yang5National Key Laboratory for Tropical Crop Breeding, School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya, ChinaNational Key Laboratory for Tropical Crop Breeding, College of Tropical Agriculture and Forestry, Hainan University, Sanya, ChinaNational Key Laboratory for Tropical Crop Breeding, School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya, ChinaNational Key Laboratory for Tropical Crop Breeding, College of Tropical Agriculture and Forestry, Hainan University, Sanya, ChinaNational Key Laboratory for Tropical Crop Breeding, School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya, ChinaNational Key Laboratory for Tropical Crop Breeding, College of Tropical Agriculture and Forestry, Hainan University, Sanya, ChinaNational Key Laboratory for Tropical Crop Breeding, School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya, ChinaYazhouwan National Laboratory, Sanya, ChinaNational Key Laboratory for Tropical Crop Breeding, School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya, ChinaNational Key Laboratory for Tropical Crop Breeding, College of Tropical Agriculture and Forestry, Hainan University, Sanya, ChinaNational Key Laboratory for Tropical Crop Breeding, School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya, ChinaMetabolite modifications play a critical role in enhancing plants’ adaptability to environmental changes and serve as a major source of functional diversity in metabolites. However, current metabolomics approaches are limited to targeted analyses of a small number of known modified metabolites and lack comprehensive, large-scale studies of plant metabolite modifications. Here, we describe a widely targeted metabolite modificomics (WTMM) strategy, developed using ultra-high-performance liquid chromatography–quadrupole linear ion trap (UHPLC-Q-Trap) and ultra-high-performance liquid chromatography–Q-Exactive Orbitrap (UHPLC-QE-Orbitrap) technologies. This strategy enables high-throughput identification and sensitive quantification of modified metabolites. Using tomato as a model, we conducted a metabolite modificomics study and constructed a WTMM database, identifying 165 novel modified metabolites. The WTMM strategy is broadly applicable and can be extended to the study of other plant species.https://bio-protocol.org/en/bpdetail?id=5259&type=0 |
| spellingShingle | Jianing Zhang Shixuan Li Yige Han Shouchuang Wang Penghui Liu Jun Yang Analysis of Modified Plant Metabolites Using Widely Targeted Metabolite Modificomics Bio-Protocol |
| title | Analysis of Modified Plant Metabolites Using Widely Targeted Metabolite Modificomics |
| title_full | Analysis of Modified Plant Metabolites Using Widely Targeted Metabolite Modificomics |
| title_fullStr | Analysis of Modified Plant Metabolites Using Widely Targeted Metabolite Modificomics |
| title_full_unstemmed | Analysis of Modified Plant Metabolites Using Widely Targeted Metabolite Modificomics |
| title_short | Analysis of Modified Plant Metabolites Using Widely Targeted Metabolite Modificomics |
| title_sort | analysis of modified plant metabolites using widely targeted metabolite modificomics |
| url | https://bio-protocol.org/en/bpdetail?id=5259&type=0 |
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