Analysis of Modified Plant Metabolites Using Widely Targeted Metabolite Modificomics
Metabolite modifications play a critical role in enhancing plants’ adaptability to environmental changes and serve as a major source of functional diversity in metabolites. However, current metabolomics approaches are limited to targeted analyses of a small number of known modified metabolites and l...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Bio-protocol LLC
2025-04-01
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| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=5259&type=0 |
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| Summary: | Metabolite modifications play a critical role in enhancing plants’ adaptability to environmental changes and serve as a major source of functional diversity in metabolites. However, current metabolomics approaches are limited to targeted analyses of a small number of known modified metabolites and lack comprehensive, large-scale studies of plant metabolite modifications. Here, we describe a widely targeted metabolite modificomics (WTMM) strategy, developed using ultra-high-performance liquid chromatography–quadrupole linear ion trap (UHPLC-Q-Trap) and ultra-high-performance liquid chromatography–Q-Exactive Orbitrap (UHPLC-QE-Orbitrap) technologies. This strategy enables high-throughput identification and sensitive quantification of modified metabolites. Using tomato as a model, we conducted a metabolite modificomics study and constructed a WTMM database, identifying 165 novel modified metabolites. The WTMM strategy is broadly applicable and can be extended to the study of other plant species. |
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| ISSN: | 2331-8325 |