Age structure and parity status determination of Afrotropical malaria vectors using MALDI-TOF MS
Abstract The age structure of a mosquito population helps estimate the proportion of vectors capable of transmitting malaria. Many malaria transmission models rely on mosquito longevity as key parameter. However, these are rarely measured in the field due to lack of a reliable and scalable age-gradi...
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Nature Portfolio
2025-05-01
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| Online Access: | https://doi.org/10.1038/s41598-025-00935-1 |
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| author | Mercy Tuwei Jonathan Karisa Caroline Kiuru Zedekiah Ondieki Tobias Odongo Martha Muturi Festus Mure Bruno Otieno Miguel Okoko Brian Bartilol Rehema Gona Kelly Ominde Luis Constantino Gildo Cole Trisa Anastácio Baltazar Candrinho Romário Armazia Claudia Alves Edith Ramaita Martin Rono Joseph Mwangangi Charles Mbogo Francisco Saute Carlos Chaccour Caroline Wanjiku Marta Maia |
| author_facet | Mercy Tuwei Jonathan Karisa Caroline Kiuru Zedekiah Ondieki Tobias Odongo Martha Muturi Festus Mure Bruno Otieno Miguel Okoko Brian Bartilol Rehema Gona Kelly Ominde Luis Constantino Gildo Cole Trisa Anastácio Baltazar Candrinho Romário Armazia Claudia Alves Edith Ramaita Martin Rono Joseph Mwangangi Charles Mbogo Francisco Saute Carlos Chaccour Caroline Wanjiku Marta Maia |
| author_sort | Mercy Tuwei |
| collection | DOAJ |
| description | Abstract The age structure of a mosquito population helps estimate the proportion of vectors capable of transmitting malaria. Many malaria transmission models rely on mosquito longevity as key parameter. However, these are rarely measured in the field due to lack of a reliable and scalable age-grading method. An accurate method could improve predictions of malaria risk and the impact assessment of interventions. This study aimed to investigate the use of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) for malaria vector age-grading using insectary-reared and wild-caught mosquitoes. Anopheles gambiae s.s. mosquitoes were reared in the insectary to different known physiological and chronological ages to evaluate if MALDI-TOF MS could be used to distinguish between different age groups. Wild mosquitoes were collected from Mozambique and Kenya and dissected to determine their parity status. Reference spectra were obtained from mosquito’s cephalothorax and used to create predictive databases which were validated using independent samples. MALDI-TOF MS identified the physiological and chronological age of insectary-reared mosquitoes with 94.52% and 77% accuracy respectively. Field-collected mosquitoes were primarily An. funestus s.s. and An. gambiae s.s. Parity prediction accuracy was between 81% and 87%. MALDI-TOF MS was able to distinguish and differentiate mosquitoes based on their age structure (chronological and physiological) and parity status. |
| format | Article |
| id | doaj-art-1fe98bfb371f4581bdf23585a127f87f |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-1fe98bfb371f4581bdf23585a127f87f2025-08-20T01:49:43ZengNature PortfolioScientific Reports2045-23222025-05-0115111110.1038/s41598-025-00935-1Age structure and parity status determination of Afrotropical malaria vectors using MALDI-TOF MSMercy Tuwei0Jonathan Karisa1Caroline Kiuru2Zedekiah Ondieki3Tobias Odongo4Martha Muturi5Festus Mure6Bruno Otieno7Miguel Okoko8Brian Bartilol9Rehema Gona10Kelly Ominde11Luis Constantino12Gildo Cole13Trisa Anastácio14Baltazar Candrinho15Romário Armazia16Claudia Alves17Edith Ramaita18Martin Rono19Joseph Mwangangi20Charles Mbogo21Francisco Saute22Carlos Chaccour23Caroline Wanjiku24Marta Maia25Kenya Medical Research Institute, Wellcome Trust Research ProgramKenya Medical Research Institute, Wellcome Trust Research ProgramCentro de Investigação em Saúde de ManhiçaKenya Medical Research Institute, Wellcome Trust Research ProgramKEMRI-CGMR CoastKenya Medical Research Institute, Wellcome Trust Research ProgramKEMRI-CGMR CoastKEMRI-CGMR CoastKEMRI-CGMR CoastKenya Medical Research Institute, Wellcome Trust Research ProgramKEMRI-CGMR CoastKenya Medical Research Institute, Wellcome Trust Research ProgramCentro de Investigação em Saúde de ManhiçaCentro de Investigação em Saúde de ManhiçaCentro de Investigação em Saúde de ManhiçaNational Malaria Control ProgramCentro de Investigação em Saúde de ManhiçaCentro de Investigação em Saúde de ManhiçaNational Malaria Control Program, Ministry of HealthKEMRI-CGMR CoastKEMRI-CGMR CoastKEMRI-CGMR CoastCentro de Investigação em Saúde de ManhiçaISGlobalKenya Medical Research Institute, Wellcome Trust Research ProgramKenya Medical Research Institute, Wellcome Trust Research ProgramAbstract The age structure of a mosquito population helps estimate the proportion of vectors capable of transmitting malaria. Many malaria transmission models rely on mosquito longevity as key parameter. However, these are rarely measured in the field due to lack of a reliable and scalable age-grading method. An accurate method could improve predictions of malaria risk and the impact assessment of interventions. This study aimed to investigate the use of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) for malaria vector age-grading using insectary-reared and wild-caught mosquitoes. Anopheles gambiae s.s. mosquitoes were reared in the insectary to different known physiological and chronological ages to evaluate if MALDI-TOF MS could be used to distinguish between different age groups. Wild mosquitoes were collected from Mozambique and Kenya and dissected to determine their parity status. Reference spectra were obtained from mosquito’s cephalothorax and used to create predictive databases which were validated using independent samples. MALDI-TOF MS identified the physiological and chronological age of insectary-reared mosquitoes with 94.52% and 77% accuracy respectively. Field-collected mosquitoes were primarily An. funestus s.s. and An. gambiae s.s. Parity prediction accuracy was between 81% and 87%. MALDI-TOF MS was able to distinguish and differentiate mosquitoes based on their age structure (chronological and physiological) and parity status.https://doi.org/10.1038/s41598-025-00935-1Anopheles gambiae s.s.An. funestus s.s.Age-gradingMALDI-TOF MSParityDetinova |
| spellingShingle | Mercy Tuwei Jonathan Karisa Caroline Kiuru Zedekiah Ondieki Tobias Odongo Martha Muturi Festus Mure Bruno Otieno Miguel Okoko Brian Bartilol Rehema Gona Kelly Ominde Luis Constantino Gildo Cole Trisa Anastácio Baltazar Candrinho Romário Armazia Claudia Alves Edith Ramaita Martin Rono Joseph Mwangangi Charles Mbogo Francisco Saute Carlos Chaccour Caroline Wanjiku Marta Maia Age structure and parity status determination of Afrotropical malaria vectors using MALDI-TOF MS Scientific Reports Anopheles gambiae s.s. An. funestus s.s. Age-grading MALDI-TOF MS Parity Detinova |
| title | Age structure and parity status determination of Afrotropical malaria vectors using MALDI-TOF MS |
| title_full | Age structure and parity status determination of Afrotropical malaria vectors using MALDI-TOF MS |
| title_fullStr | Age structure and parity status determination of Afrotropical malaria vectors using MALDI-TOF MS |
| title_full_unstemmed | Age structure and parity status determination of Afrotropical malaria vectors using MALDI-TOF MS |
| title_short | Age structure and parity status determination of Afrotropical malaria vectors using MALDI-TOF MS |
| title_sort | age structure and parity status determination of afrotropical malaria vectors using maldi tof ms |
| topic | Anopheles gambiae s.s. An. funestus s.s. Age-grading MALDI-TOF MS Parity Detinova |
| url | https://doi.org/10.1038/s41598-025-00935-1 |
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