Dissemination of IncQ1 Plasmids Harboring NTE<sub>KPC</sub>-IId in a Brazilian Hospital
KPC is a clinically significant serine carbapenemase in most countries, and its rapid spread threatens global public health. <i>bla</i><sub>KPC</sub> transmission is commonly mediated by Tn<i>4401</i> transposons. The <i>bla</i><sub>KPC</sub&g...
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2025-01-01
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| author | Camila Maria dos Santos Boralli Julian Andres Paganini Rodrigo Silva Meneses Camila Pacheco Silveira Martins da Mata Edna Marilea Meireles Leite Anita C. Schürch Fernanda L. Paganelli Rob J. L. Willems Ilana L. B. C. Camargo |
| author_facet | Camila Maria dos Santos Boralli Julian Andres Paganini Rodrigo Silva Meneses Camila Pacheco Silveira Martins da Mata Edna Marilea Meireles Leite Anita C. Schürch Fernanda L. Paganelli Rob J. L. Willems Ilana L. B. C. Camargo |
| author_sort | Camila Maria dos Santos Boralli |
| collection | DOAJ |
| description | KPC is a clinically significant serine carbapenemase in most countries, and its rapid spread threatens global public health. <i>bla</i><sub>KPC</sub> transmission is commonly mediated by Tn<i>4401</i> transposons. The <i>bla</i><sub>KPC</sub> gene has also been found in <i>non-Tn4401 elements</i> (NTE<sub>KPC</sub>). To fill the gap in the understanding of the stability and dissemination of NTE<sub>KPC</sub>-carrying plasmids, we selected and characterized carbapenem-resistant bacteria isolated between 2009 and 2016 from a hospital for a retrospective study of their plasmids conjugation capacity, impact on fitness, and replication in different species. Different clones were selected using PFGE, and their genomes were sequenced using Illumina and Oxford Nanopore methods. Minimum inhibitory concentrations (MICs) were determined by broth microdilution. Plasmid copy numbers (PCNs) were determined using qPCR. Doubling time was used to analyze fitness change. Most isolates (67%, 33/49) carried <i>bla</i><sub>KPC</sub>, of which 85% presented <i>bla</i><sub>KPC</sub> in a NTE<sub>KPC</sub>. The 25 isolates selected presented the <i>bla</i><sub>KPC</sub> gene in NTE<sub>KPC</sub>-IId in IncQ1-type plasmids, showing multispecies dissemination. IncQ1 plasmids were mobilizable and PCN seemed to be directly linked to the species, presenting a high-copy number, mainly in <i>K. pneumoniae</i>. No relationship was observed between IncQ1 PCN and carbapenems MIC values. IncQ1 and a conjugative plasmid from <i>K. pneumoniae</i> BHKPC10 were transferred to <i>E. coli</i> J53 without fitness changes, and MIC values were maintained for carbapenems despite the low transconjugant PCN. In addition to IncQ1 with NTE<sub>KPC</sub>, <i>Enterobacter cloacae</i> BHKPC28 contained the <i>mcr-9</i> gene in an IncHI2/IncHI2A conjugative plasmid, which may help the mobility of IncQ1 and the dissemination of two resistance determinants to last-resort antibiotics. Understanding the interaction between plasmids and high-risk lineages can help develop new therapies to prevent the dissemination of resistance traits. |
| format | Article |
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| institution | Kabale University |
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| publishDate | 2025-01-01 |
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| spelling | doaj-art-1fe8d457d0c2468e83b4a0d1102e54672025-01-24T13:42:57ZengMDPI AGMicroorganisms2076-26072025-01-0113118010.3390/microorganisms13010180Dissemination of IncQ1 Plasmids Harboring NTE<sub>KPC</sub>-IId in a Brazilian HospitalCamila Maria dos Santos Boralli0Julian Andres Paganini1Rodrigo Silva Meneses2Camila Pacheco Silveira Martins da Mata3Edna Marilea Meireles Leite4Anita C. Schürch5Fernanda L. Paganelli6Rob J. L. Willems7Ilana L. B. C. Camargo8Laboratório de Epidemiologia e Microbiologia Moleculares—LEMiMo, Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos 13563-120, SP, BrazilUniversity Medical Center Utrecht, 3584 CX Utrecht, The NetherlandsUniversity Medical Center Utrecht, 3584 CX Utrecht, The NetherlandsRisoleta Tolentino Neves Hospital, Belo Horizonte 31744-012, MG, BrazilRisoleta Tolentino Neves Hospital, Belo Horizonte 31744-012, MG, BrazilUniversity Medical Center Utrecht, 3584 CX Utrecht, The NetherlandsUniversity Medical Center Utrecht, 3584 CX Utrecht, The NetherlandsUniversity Medical Center Utrecht, 3584 CX Utrecht, The NetherlandsLaboratório de Epidemiologia e Microbiologia Moleculares—LEMiMo, Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos 13563-120, SP, BrazilKPC is a clinically significant serine carbapenemase in most countries, and its rapid spread threatens global public health. <i>bla</i><sub>KPC</sub> transmission is commonly mediated by Tn<i>4401</i> transposons. The <i>bla</i><sub>KPC</sub> gene has also been found in <i>non-Tn4401 elements</i> (NTE<sub>KPC</sub>). To fill the gap in the understanding of the stability and dissemination of NTE<sub>KPC</sub>-carrying plasmids, we selected and characterized carbapenem-resistant bacteria isolated between 2009 and 2016 from a hospital for a retrospective study of their plasmids conjugation capacity, impact on fitness, and replication in different species. Different clones were selected using PFGE, and their genomes were sequenced using Illumina and Oxford Nanopore methods. Minimum inhibitory concentrations (MICs) were determined by broth microdilution. Plasmid copy numbers (PCNs) were determined using qPCR. Doubling time was used to analyze fitness change. Most isolates (67%, 33/49) carried <i>bla</i><sub>KPC</sub>, of which 85% presented <i>bla</i><sub>KPC</sub> in a NTE<sub>KPC</sub>. The 25 isolates selected presented the <i>bla</i><sub>KPC</sub> gene in NTE<sub>KPC</sub>-IId in IncQ1-type plasmids, showing multispecies dissemination. IncQ1 plasmids were mobilizable and PCN seemed to be directly linked to the species, presenting a high-copy number, mainly in <i>K. pneumoniae</i>. No relationship was observed between IncQ1 PCN and carbapenems MIC values. IncQ1 and a conjugative plasmid from <i>K. pneumoniae</i> BHKPC10 were transferred to <i>E. coli</i> J53 without fitness changes, and MIC values were maintained for carbapenems despite the low transconjugant PCN. In addition to IncQ1 with NTE<sub>KPC</sub>, <i>Enterobacter cloacae</i> BHKPC28 contained the <i>mcr-9</i> gene in an IncHI2/IncHI2A conjugative plasmid, which may help the mobility of IncQ1 and the dissemination of two resistance determinants to last-resort antibiotics. Understanding the interaction between plasmids and high-risk lineages can help develop new therapies to prevent the dissemination of resistance traits.https://www.mdpi.com/2076-2607/13/1/180<i>bla</i><sub>KPC</sub>NTE<sub>KPC</sub>-IIdplasmidIncQ1conjugation rateplasmid copy number |
| spellingShingle | Camila Maria dos Santos Boralli Julian Andres Paganini Rodrigo Silva Meneses Camila Pacheco Silveira Martins da Mata Edna Marilea Meireles Leite Anita C. Schürch Fernanda L. Paganelli Rob J. L. Willems Ilana L. B. C. Camargo Dissemination of IncQ1 Plasmids Harboring NTE<sub>KPC</sub>-IId in a Brazilian Hospital Microorganisms <i>bla</i><sub>KPC</sub> NTE<sub>KPC</sub>-IId plasmid IncQ1 conjugation rate plasmid copy number |
| title | Dissemination of IncQ1 Plasmids Harboring NTE<sub>KPC</sub>-IId in a Brazilian Hospital |
| title_full | Dissemination of IncQ1 Plasmids Harboring NTE<sub>KPC</sub>-IId in a Brazilian Hospital |
| title_fullStr | Dissemination of IncQ1 Plasmids Harboring NTE<sub>KPC</sub>-IId in a Brazilian Hospital |
| title_full_unstemmed | Dissemination of IncQ1 Plasmids Harboring NTE<sub>KPC</sub>-IId in a Brazilian Hospital |
| title_short | Dissemination of IncQ1 Plasmids Harboring NTE<sub>KPC</sub>-IId in a Brazilian Hospital |
| title_sort | dissemination of incq1 plasmids harboring nte sub kpc sub iid in a brazilian hospital |
| topic | <i>bla</i><sub>KPC</sub> NTE<sub>KPC</sub>-IId plasmid IncQ1 conjugation rate plasmid copy number |
| url | https://www.mdpi.com/2076-2607/13/1/180 |
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